Unraveling enhanced membrane lipid biosynthesis in <italic style="font-style: italic">Chlamydomonas reinhardtii</italic> starchless mutant <italic style="font-style: italic">sta6</italic> by using an electrospray ionization mass spectrometry-based lipidomics method

Zhengrong ZANG; Yanhua LI; Qiang HU; Danxiang HAN

doi:10.1007/s00343-019-9138-1

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Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method

Updated：2023-05-19

- Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method
- Journal of Oceanology and Limnology Vol. 38, Issue 3, Pages: 783-794(2020)
- Affiliations：
  
  1.Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.Key Laboratory for Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  5.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  6.Beijing Key Laboratory of Algae Biomass, Microalgae Biotechnology Center, SDIC Biotech Investment Co. Ltd., State Development & Investment Corp., Beijing 100142, China
- Author bio：
  
  HAN Danxiang, danxianghan@ihb.ac.cn
- Funds：
- DOI：10.1007/s00343-019-9138-1
  CLC：
- Received：17 May 2019，
  
  Accepted：02 July 2019，
  
  Online First：22 August 2019，
  
  Published：2020-03
- Accepted：
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Zhengrong ZANG, Yanhua LI, Qiang HU, et al. Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method[J]. Journal of Oceanology and Limnology, 2020, 38(3): 783-794.
DOI：

Zhengrong ZANG, Yanhua LI, Qiang HU, et al. Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method[J]. Journal of Oceanology and Limnology, 2020, 38(3): 783-794. DOI： 10.1007/s00343-019-9138-1.

摘要

Abstract

The unicellular green alga

Chlamydomonas reinhardtii

a well-established model organism

has been widely used in dissecting glycerolipid metabolism in oxygenating photosynthetic organisms. In previous studies

it has been found that shunting carbon precursors from the starch synthesis pathway can lead to a 10-fold increase in TAG content as compared to the wild type

but it is unknown whether inactivation of AGPase may affect membrane lipids biosynthesis. The study aims to investigate global changes in lipid metabolism and homeostasis in the starchless mutant

reinhardtii sta6

. By utilizing an electrospray ionization/mass spectrometry (ESI/MS)-based lipidomics approach

a total of 105 membrane lipid molecules of

reinhardtii

were resolved

including 16 monogalactosyldiacylglycerol (MGDG)

16 digalactosyldiacylglycerol (DGDG)

11 phosphatidylglycerol (PG)

6 sulfoquinovosyldiacylglycerol (SQDG)

49 diacylglyceryl-

-trimethylhomoserine (DGTS)

2 phosphatidylethanolamine (PE)

and 5 phosphatidylinositol (PI) molecules. The quantitative results indicated that the membrane lipid profiles were similar between the two

reinhardtii

strains grown under both low- and high-light conditions

but the cellular contents of a great number of lipids were altered in

sta6

due to the defect in starch biosynthesis. Under low-light conditions

sta6

accumulated more PI

MGDG

DGDG but less amounts of DGTS as compared to WT. Under high light

ta6

cells contained higher content membrane lipids than cc-124

except for PG

which is more or less similar in both strains. Our results demonstrate that the cellular membrane lipid homeostasis underwent profound changes in the starchless mutant

and thereby its physiological impact remains to be explored.

关键词

Keywords

references

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Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method

DOI：10.1007/s00343-019-9138-1

摘要

Abstract

关键词

Keywords

references