Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

Pei HONG; Shihao GONG; Chunbo WANG; Yilin SHU; Xingqiang WU; Cuicui TIAN; Omondi DONDE Oscar; Pei CAI; Huaming WU; Bangding XIAO

doi:10.1007/s00343-019-9103-z

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Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

Ecology | Updated：2023-05-19

- Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface
- Journal of Oceanology and Limnology Vol. 38, Issue 3, Pages: 733-744(2020)
- Affiliations：
  
  1.Key Laboratory of Algal Biology of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.College of Life Sciences, Anhui Normal University, Wuhu 241000, China
- Author bio：
  
  XIAO Bangding, bdxiao@ihb.ac.cn
- Funds：
- DOI：10.1007/s00343-019-9103-z
  CLC：
- Received：18 April 2019，
  
  Accepted：24 July 2019，
  
  Online First：19 August 2019，
  
  Published：2020-05
- Accepted：
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Pei HONG, Shihao GONG, Chunbo WANG, et al. Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface[J]. Journal of Oceanology and Limnology, 2020, 38(3): 733-744.
DOI：

Pei HONG, Shihao GONG, Chunbo WANG, et al. Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface[J]. Journal of Oceanology and Limnology, 2020, 38(3): 733-744. DOI： 10.1007/s00343-019-9103-z.

摘要

Abstract

At present

the understanding of the dynamics of denitrifiers at different dissolved oxygen (DO) layers under organic carbon consumption within the surface sediments remains inadequate. In this study

high-throughput sequencing and quantitative PCR targeting

nirS

gene were used to analyze the denitrifier abundance dynamics

community composition

and structure for aerobic (DO 0.5-6.9 mg/L)

hypoxic-anoxic (DO 0-0.5 mg/L)

and anoxic (DO 0 mg/L) layers in surface sediments under organic carbon consumption. Based on the analysis of

nirS

gene abundance

the values of denitrifying bacteria decreased with organic carbon consumption at different DO layers. When the bacterial species abundance at the genus level were compared between the high-carbon and low-carbon sediments

there was significant increase in 6 out of 36

7 out of 36 and 6 out of 36 genera respectively for the aerobic

hypoxic-anoxic and anoxic layers. On the other hand

14 out of 36

9 out of 36 and 15 out of 36 genera showed significant decrease in bacterial species abundance respectively for the aerobic

hypoxic-anoxic and anoxic layers. Additionally

14 out of 36

20 out of 36

and 15 out of 36 genera had no change in bacterial species abundance respectively for the aerobic

hypoxic-anoxic

and anoxic layers. This indicates that the carbon utilization ability of different denitrifiers on each DO layers was generally different from each other. Diversity of denitrifying bacteria also presented significant differences in different DO layers between the high- and low-carbon content sediment layers. Moreover

under the high-carbon and low-carbon content

the abundance of

nirS

gene showed a high peak within the hypoxic-anoxic regions

suggesting that this region might be the main distribution area for the denitrifying bacteria within the surface sediments. Furthermore

community of unique denitrifiers occurred in different DO layers and the adaptive changes of the denitrifier community followed the organic carbon consumption.

关键词

Keywords

references

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