Establishment and characterization of a new cell line derived from half-smooth tongue sole <italic style="font-style: italic">Cynoglossus semilaevis</italic> kidney

Yanan LOU; Bin SUN; Lina ZHANG; Yong LI; Peng XIAO

doi:10.1007/s00343-019-9084-y

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Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney

Aquaculture and Fisheries | Updated：2023-04-27

- Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney
- Journal of Oceanology and Limnology Vol. 38, Issue 2, Pages: 560-570(2020)
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Tianjin Weishan Road Middle School, Tianjin 300222, China
- Author bio：
  
  XIAO Peng, E-mail:xiaopeng@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-019-9084-y
  CLC：
- Received：26 March 2019，
  
  Accepted：05 May 2019，
  
  Online First：29 July 2019，
  
  Published：2020-03
- Accepted：
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Yanan LOU, Bin SUN, Lina ZHANG, et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. Journal of Oceanology and Limnology, 2020, 38(2): 560-570.
DOI：

Yanan LOU, Bin SUN, Lina ZHANG, et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. Journal of Oceanology and Limnology, 2020, 38(2): 560-570. DOI： 10.1007/s00343-019-9084-y.

摘要

Abstract

In this study

we established a new cell line (

Cynoglossus semilaevis

kidney cells

CSK) from kidney of the half-smooth tongue sole (

Cynoglossus semilaevis

). The cells were subcultured over 1 000 days and passaged for more than 100 times. Additionally

CSK cells were optimally maintained in Dulbecco's modified Eagle medium nutrient mixture F-12 supplemented with HEPES

antibiotics

fetal bovine serum

2-mercaptoethanol

and basic fibroblast growth factor. The optimum growth temperature for CSK cells was 25℃

and the cells showed a fibroblast-like phenotype. Chromosome analysis revealed that CSK cells had a normal diploid karyotype with 2n=42. CSK cells were susceptible to Grouper nervous necrosis virus (NNV)

and cytopathic effects were observed at 3-5 days postinfection. The NNV sensitivity of CSK cells was related to the high abundance of virions in the cytoplasm

as observed by electron microscopy. Additionally

CSK cells could be successfully transfected with a green fluorescent protein reporter plasmid

and fluorescent signals were easily observed. Finally

immunocytochemistry analysis showed that CSK cells were supporting cells. Overall

we established this new cell line

which may have potential applications in the identification of viral pathogens affecting the half-smooth tongue sole.

关键词

Keywords

references

A S Barman , K K Lal , G Rathore , V Mohindra , R K Singh , A Singh , P Khare , B Lal . Derivation and characterization of a es-like cell line from indian catfish heteropneustes fossilis blastulas . Scientific World Journal , 2014 . 2014 427497 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Doaj000003700534 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Doaj000003700534 , .

N C Bols , L E J Lee . Technology and uses of cell cultures from the tissues and organs of bony fish . Cytotechnology , 1991 . 6 ( 3 ): 163 - 187 . DOI: 10.1007/BF00624756 http://doi.org/10.1007/BF00624756 .

H Chen , Y Yi , M Chen , X Yang . Studies on the developmental potentiality of cultured cell nuclei of fish . International Journal of Biological Sciences , 2010 . 6 ( 2 ): 192 - 198 . http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_5d666cdf151431f95a8157cba3477878 http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_5d666cdf151431f95a8157cba3477878 , .

Chen J. 2012. Bacterial Diseases and Control of Tongue Fish( Cynoglossus semilaevis ) in Recirculating Culture System. Shanghai Ocean University, Shanghai. 85p. (in Chinese with English abstract)

Z Q Chen , Z X Yao , M Lin , J B Chang . Characterization of pathogenic bacteria Vibrio rotiferianus isolated from Cynoglossus semilaevis Günther . Biotechnology Bulletin , 2012 . ( 6 ): 147 - 153 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=swjstb201206026 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=swjstb201206026 , .

S C Chi , Y C Wu , T M Cheng . Persistent infection of betanodavirus in a novel cell line derived from the brain tissue of barramundi Lates calcarifer . Diseases of Aquatic Organisms , 2005 . 65 ( 2 ): 91 - 98 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=7e2d9efd665f607169c7d2ccbbe41cda http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=7e2d9efd665f607169c7d2ccbbe41cda , .

L W Clem , J E Bly , M Wilson , V G Chinchar , T Stuge , K Barker , C Luft , M Rycyzyn , R J Hogan , T Van Lopik , N W Miller . Fish immunology, the utility of immortalized lymphoid cells-a mini review . Veterinary Immunology and Immunopathology , 1996 . 54 ( 1-4 ): 137 - 144 . DOI: 10.1016/S0165-2427(96)05682-6 http://doi.org/10.1016/S0165-2427(96)05682-6 .

B Crespo , S Zanuy , A Gómez . Development of an in vitro system for functional studies of ovarian follicular cells in European sea bass ( Dicentrarchus labrax ) . Cytotechnology , 2013 . 65 ( 2 ): 273 - 286 . DOI: 10.1007/s10616-012-9484-8 http://doi.org/10.1007/s10616-012-9484-8 .

L C Fan , P Collodi . Progress towards cell-mediated gene transfer in zebrafish . Briefings in Functional Genomics & Proteomics , 2002 . 1 ( 2 ): 131 - 138 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HighWire000002184253 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HighWire000002184253 , .

Freshney R I. 2010. Culture of Animal Cells. 6 th edn. New York: John Wiley & Sons, p.239-268.

A A A Gamil , Ø Evensen , S Mutoloki . Infection profiles of selected aquabirnavirus isolates in CHSE cells . PLoS One , 2015 . 10 ( 8 ): e134173 http://cn.bing.com/academic/profile?id=8f50bc1452dd7ef9c412f8e5b22d1d8a&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=8f50bc1452dd7ef9c412f8e5b22d1d8a&encoded=0&v=paper_preview&mkt=zh-cn , .

G F Gerlach , R A Wingert . Kidney organogenesis in the zebrafish:insights into vertebrate nephrogenesis and regeneration . Wiley Interdisciplinary Reviews:Developmental Biology , 2013 . 2 ( 5 ): 559 - 585 . DOI: 10.1002/wdev.92 http://doi.org/10.1002/wdev.92 .

T Isshiki , T Nagano , K Kanehira , S Suzuki . Distribution of marine birnavirus in cultured marine fish species from Kagawa Prefecture, Japan . Journal of Fish Diseases , 2004 . 27 ( 2 ): 89 - 98 . DOI: 10.1111/j.1365-2761.2003.00518.x http://doi.org/10.1111/j.1365-2761.2003.00518.x .

K R John , R H Richards . Characteristics of a new birnavirus associated with a warm-water fish cell line . Journal of General Virology , 1999 . 80 ( 8 ): 2 061 - 2 065 . DOI: 10.1099/0022-1317-80-8-2061 http://doi.org/10.1099/0022-1317-80-8-2061 .

W S Lakra , T R Swaminathan , K P Joy . Development, characterization, conservation and storage of fish cell lines:a review . Fish Physiology and Biochemistry , 2011 . 37 ( 1 ): 1 - 20 . http://cn.bing.com/academic/profile?id=0f9552e448fe627bab5f916e97b29db3&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=0f9552e448fe627bab5f916e97b29db3&encoded=0&v=paper_preview&mkt=zh-cn , .

A Levan , K Fredga , A A Sandberg . Nomenclature for centromeric position on chromosomes . Hereditas , 1964 . 52 ( 2 ): 201 - 220 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1111/j.1601-5223.1964.tb01953.x http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1111/j.1601-5223.1964.tb01953.x , .

J Li , C Shi , J Huang , W G Geng , S Q Wang , Z D Su . Complete genome sequence of a betanodavirus isolated from half-smooth tongue sole ( Cynoglossus semilaevis ) . Genome Announcements , 2014 . 2 ( 6 ): e01312 - 14 . http://europepmc.org/article/PMC/4271161 http://europepmc.org/article/PMC/4271161 , .

X L Liao , H Y Ma , G B Xu , C W Shao , Y S Tian , X S Ji , J F Yang , S L Chen . Construction of a genetic linkage map and mapping of a female-specific DNA marker in half-smooth tongue sole ( Cynoglossus semilaevis ) . Marine Biotechnology , 2009 . 11 ( 6 ): 699 - 709 . DOI: 10.1007/s10126-009-9184-3 http://doi.org/10.1007/s10126-009-9184-3 .

Y Liu , S L Chen , F T Gao , L Meng , Q M Hu , W T Song , C W Shao , W Q Lv . SCAR-transformation of sex-specific SSR marker and its application in half-smooth tongue sole( Cynoglossus semiliaevis ) . Journal of Agricultural Biotechnology , 2014 . 22 ( 6 ): 787 - 792 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=nyswjsxb201406015 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=nyswjsxb201406015 , .

Luo Z, Li J, Zhang Z G, Hao S, Bai X H, You H Z, Mo Z L, Feng S M. 2018. Mycobacterium marinum is the causative agent of splenic and renal granulomas in half-smooth tongue sole ( Cynoglossus semilaevis Günther) in China.Aquaculture, 490: 203-207.

B L Nicholson , D J Danner , J L Wu . Three new continuous cell lines from marine fishes of Asia . In Vitro Cellular & Developmental Biology , 1987 . 23 ( 3 ): 199 - 204 . http://cn.bing.com/academic/profile?id=35c05fd15ad94bc7f765e2adce923ffe&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=35c05fd15ad94bc7f765e2adce923ffe&encoded=0&v=paper_preview&mkt=zh-cn , .

G C Ren , S Chen , Z X Sha . Development and characterization of a liver cell line from half-smooth tongue-sole ( Cynoglossus semilaevis ) . Chinese High Technology Letters , 2008 . 18 ( 6 ): 657 - 660 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gjstx98200806019 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gjstx98200806019 , .

L Rocco , I V Valentino , G Scapigliati , V Stingo . RAPDPCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax . Cytotechnology , 2014 . 66 ( 3 ): 383 - 393 . DOI: 10.1007/s10616-013-9586-y http://doi.org/10.1007/s10616-013-9586-y .

M Rode , T Berg , T Gjøen . Effect of temperature on endocytosis and intracellular transport in the cell line SHK-1 derived from salmon head kidney . Comparative Biochemistry and Physiology Part A:Physiology , 1997 . 117 ( 4 ): 531 - 537 . DOI: 10.1016/S0300-9629(96)00414-8 http://doi.org/10.1016/S0300-9629(96)00414-8 .

Z X Sha , G C Ren , X L Wang , N Wang , S L Chen . Development and characterization of a cell line from the embryos of half smooth tongue sole ( Cynoglossus semilaevis ) . Acta Oceanologica Sinica , 2010 . 29 ( 2 ): 81 - 87 . DOI: 10.1007/s13131-010-0025-z http://doi.org/10.1007/s13131-010-0025-z .

C W Shao , P F Wu , X L Wang , Y S Tian , S L Chen . Comparison of chromosome preparation methods for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis . Micron , 2010 . 41 ( 1 ): 47 - 50 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ceb73d15796f5c9acb32f1f480418cfc http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ceb73d15796f5c9acb32f1f480418cfc , .

A Sun , S L Chen , F T Gao , H L Li , X F Liu , N Wang , Z X Sha . Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale . Fish Physiology and Biochemistry , 2015 . 41 ( 3 ): 673 - 683 . DOI: 10.1007/s10695-015-0037-x http://doi.org/10.1007/s10695-015-0037-x .

X L Wang , N Wang , Z X Sha , S L Chen . Establishment, characterization of a new cell line from heart of half smooth tongue sole ( Cynoglossus semilaevis ) . Fish Physiology and Biochemistry , 2010 . 36 ( 4 ): 1 181 - 1 189 . DOI: 10.1007/s10695-010-9396-5 http://doi.org/10.1007/s10695-010-9396-5 .

Y Wang , Y Han , Y Li , J X Chen , X H Zhang . Isolation of photobacterium damselae subsp . piscicida from diseased tongue sole (Cynoglossus semilaevis Günther) in China.Acta Microbiologica Sinica , 2007 . 47 ( 5 ): 763 - 768 . http://en.cnki.com.cn/Article_en/CJFDTOTAL-WSXB200705006.htm http://en.cnki.com.cn/Article_en/CJFDTOTAL-WSXB200705006.htm , .

C F Webb , M L Ratliff , R Powell , C R Wirsig-Wiechmann , O Lakiza , T Obara . A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures . Biochemical and Biophysical Research Communications , 2015 . 463 ( 4 ): 1 334 - 1 340 . DOI: 10.1016/j.bbrc.2015.06.130 http://doi.org/10.1016/j.bbrc.2015.06.130 .

K Wolf , M C Quimby . Established eurythermic line of fish cells in vitro . Science , 1962 . 135 ( 3508 ): 1 065 - 1 066 . DOI: 10.1126/science.135.3508.1065 http://doi.org/10.1126/science.135.3508.1065 .

P Xiao , M Jiang , Y Liu , M Sun , L Zhang , L Jie , G Li , Z Mo . Splenic necrosis signs and pathogen detection in cultured half-smooth tongue sole, Cynoglossus semilaevis Günther . Journal of Fish Diseases , 2015 . 38 ( 1 ): 103 - 106 . DOI: 10.1111/jfd.12213 http://doi.org/10.1111/jfd.12213 .

B Zhang , X L Wang , Z X Sha , C G Yang , S S Liu , N Wang , S L Chen . Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis . International Journal of Biological Sciences , 2011 . 7 ( 4 ): 452 - 459 . DOI: 10.7150/ijbs.7.452 http://doi.org/10.7150/ijbs.7.452 .

X J Zhang , G M Qin , B L Yan , J Xu , K R Bi , L Qin . Phenotypic and molecular characterization of pathogenic Listonella anguillarum isolated from half-smooth tongue sole Cynoglossus semilaevis . Acta Oceanologica Sinica , 2009 . 31 ( 5 ): 112 - 122 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyxb200905012 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyxb200905012 , .

Y Zheng , N Wang , M S Xie , Z X Sha , S L Chen . Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole ( Cynoglossus semilaevis ) . Fish Physiology and Biochemistry , 2012 . 38 ( 6 ): 1 635 - 1 643 . DOI: 10.1007/s10695-012-9660-y http://doi.org/10.1007/s10695-012-9660-y .

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Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney

DOI：10.1007/s00343-019-9084-y

摘要

Abstract

关键词

Keywords

references