Distinct influence of trimethylamine <italic style="font-style: italic">N</italic>-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

Chan ZHANG; Wei-jia ZHANG; Qunjian YIN; Xuegong LI; Xiaoqing QI; Long-fei WU

doi:10.1007/s00343-019-9076-y

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Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

Ecology | Updated：2023-04-27

- Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria
- Journal of Oceanology and Limnology Vol. 38, Issue 2, Pages: 364-377(2020)
- Affiliations：
  
  1.Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS-Sanya, China
  4.Aix Marseille Univ, CNRS, LCB, Marseille F-13402, France
- Author bio：
  
  ZHANG Wei-jia, wzhang@idsse.ac.cn
- Funds：
- DOI：10.1007/s00343-019-9076-y
  CLC：
- Received：21 March 2019，
  
  Accepted：30 April 2019，
  
  Online First：13 June 2019，
  
  Published：2020-02
- Accepted：
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Chan ZHANG, Wei-jia ZHANG, Qunjian YIN, et al. Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria[J]. Journal of Oceanology and Limnology, 2020, 38(2): 364-377.
DOI：

Chan ZHANG, Wei-jia ZHANG, Qunjian YIN, et al. Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria[J]. Journal of Oceanology and Limnology, 2020, 38(2): 364-377. DOI： 10.1007/s00343-019-9076-y.

摘要

Abstract

Trimethylamine

-oxide (TMAO) is one of the most important nutrients for bacteria in the deep-sea environment and is capable of improving pressure tolerance of certain bacterial strains. To assess the impact of TMAO on marine microorganisms

especially those dwelling in the deep-sea environment

we analyzed the bacterial community structure of deep-sea sediments after incubated under different conditions. Enrichments at 50 MPa and 0.1 MPa revealed that TMAO imposed a greater influence on bacterial diversity and community composition at atmospheric pressure condition than that under high hydrostatic pressure (HHP). We found that pressure was the primary factor that determines the bacterial community. Meanwhile

in total

238 bacterial strains were isolated from the enrichments

including 112 strains affiliated to 16 genera of 4 phyla from the Yap Trench and 126 strains affiliated to 11 genera of 2 phyla from the Mariana Trench. Treatment of HHP reduced both abundance and diversity of isolates

while the presence of TMAO mainly affected the diversity of isolates obtained. In addition

certain genera were isolated only when TMAO was supplemented. Taken together

we demonstrated that pressure primarily defines the bacterial community and culturable bacterial isolates. Furthermore

we showed for the first time that TMAO had distinct influences on bacterial community depending on the pressure condition. The results enriched the understanding of the significance of TMAO in bacterial adaptation to the deep-sea environment.

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Related Author

Qunjian YIN

Weijia ZHANG

Xuegong LI

Lihong ZHOU

Xiaoqing QI

Chan ZHANG

Long-Fei WU

Zhenxiang LI

Related Institution

Laboratory of Deep-sea Microbial Cell Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences

International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS--Qingdao-Sanya

CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences

AMU, LCB UMR 7283, CNRS

College of Life Science and Technology, Harbin Normal University

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Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

DOI：10.1007/s00343-019-9076-y

摘要

Abstract

关键词

Keywords

references