bHLH genes polymorphisms and their association with growth traits in the Pacific oyster <italic style="font-style: italic">Crassostrea gigas</italic>

Na CHEN; Li LI; Chenghua LI; Zhihua LIN; Jie MENG; Sheng LIU; Kai SONG; Yongbo BAO

doi:10.1007/s00343-019-9070-4

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bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas

Aquaculture and Fisheries | Updated：2023-05-19

- bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas
- Journal of Oceanology and Limnology Vol. 38, Issue 3, Pages: 862-868(2020)
- Affiliations：
  
  1.Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315121, China
  2.School of Marine Sciences, Ningbo University, Ningbo 315211, China
  3.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  BAO Yongbo, bobbao2001@gmail.com
- Funds：
- DOI：10.1007/s00343-019-9070-4
  CLC：
- Received：16 March 2019，
  
  Accepted：02 July 2019，
  
  Online First：19 August 2019，
  
  Published：2020-05
- Accepted：
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Na CHEN, Li LI, Chenghua LI, et al. bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas[J]. Journal of Oceanology and Limnology, 2020, 38(3): 862-868.
DOI：

Na CHEN, Li LI, Chenghua LI, et al. bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas[J]. Journal of Oceanology and Limnology, 2020, 38(3): 862-868. DOI： 10.1007/s00343-019-9070-4.

摘要

Abstract

The basic helix-loop-helix (bHLH) genes encode a large superfamily of transcription factors in the Pacific oyster (

Crassostrea gigas

)

and play a very important role in regulation of growth and development. To investigate the oyster growth traits and the associations with bHLH genes variations

we analyzed the gene polymorphisms-traits association in a wild population

and confirmed the results in another independent wild population by targeted gene re-sequencing and SNPshot analysis. After screening the single nucleotide polymorphisms (SNPs) in three candidate genes of the bHLH family (88 bHLH genes in two wild oyster populations in total)

we identified the allele CgLoblHLH4-T/G located in the exon of the CgLoblHLH4 gene. This allele is a non-synonymous mutation (Phe/Leu) with an extremely significant association with shell width (

0.01) and allele G is beneficial to shell width. This SNP on the CgLoblHLH4 gene might have a potential value as a genetic marker of growth traits that could be used in breeding in

gigas

in the future.

关键词

Keywords

references

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bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas

DOI：10.1007/s00343-019-9070-4

摘要

Abstract

关键词

Keywords

references