Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail <italic style="font-style: italic">Semisulcospira coreana</italic> and cross-species amplification using next-generation sequencing

YeonJung PARK; Mi Nan LEE; Eun-Mi KIM; JungYoun PARK; JaeKoo NOH; Tae-Jin CHOI; Jung-Ha KANG

doi:10.1007/s00343-019-9058-0

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Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

Updated：2023-04-27

- Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing
- Journal of Oceanology and Limnology Vol. 38, Issue 2, Pages: 503-508(2020)
- Affiliations：
  
  1.Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea
  2.Department of Microbiology, Pukyoung National University, Busan 48513, Republic of Korea
- Author bio：
  
  Jung-Ha KANG, ocean0629@korea.kr
- Funds：
- DOI：10.1007/s00343-019-9058-0
  CLC：
- Received：12 March 2019，
  
  Accepted：11 April 2019，
  
  Online First：04 June 2019，
  
  Published：2020-03
- Accepted：
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YeonJung PARK, Mi Nan LEE, Eun-Mi KIM, et al. Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing[J]. Journal of Oceanology and Limnology, 2020, 38(2): 503-508.
DOI：

YeonJung PARK, Mi Nan LEE, Eun-Mi KIM, et al. Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing[J]. Journal of Oceanology and Limnology, 2020, 38(2): 503-508. DOI： 10.1007/s00343-019-9058-0.

摘要

Abstract

Korean freshwater snails of the genus

Semisulcospira

are widely distributed across East Asia. It has been a very popular nutritional food in Korea

and is an ecologically important water quality indicator because it lives only in clean water. However

no microsatellite markers have been generated to study the population genetic diversity of this genus. In the present study

we developed and characterized 18 novel microsatellite loci from

Semisulcospira coreana

genomic DNA. The microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in

S. coreana

. In addition

we also tested the cross-species transferability of the microsatellite markers in four additional

Semisulcospira

spp. We identified 18 polymorphic loci and the number of alleles per loci

and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902

respectively. The observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924

respectively. According to the analysis of the cross-species transferability of these markers

four species

S. forticosta

S. gottschei

S. tegulata

and

S. libertina

showed a very high transferability (80%-85%). These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.

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Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

DOI：10.1007/s00343-019-9058-0

摘要

Abstract

关键词

Keywords

references