Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot <italic style="font-style: italic">Scophthalmus maximus</italic> responded to hypo-saline seawater

Wenxiao CUI; Aijun MA; Zhihui HUANG; Xin'an WANG; Zhibin SUN; Zhifeng LIU; GWei ZHAN; Jingkun YANG; Jinsheng ZHANG; Jiangbo QU

doi:10.1007/s00343-019-9056-2

Your Location：

Home >

Browse articles >

Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

Updated：2023-04-27

- Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater
- Journal of Oceanology and Limnology Vol. 38, Issue 2, Pages: 467-479(2020)
- Affiliations：
  
  1.College of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
  2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China
  4.Yantai Tianyuan Aquatic Limited Corporation, Yantai 264003, China
- Author bio：
  
  MA Aijun, maaj@ysfri.ac.cn
- Funds：
- DOI：10.1007/s00343-019-9056-2
  CLC：
- Received：08 March 2019，
  
  Accepted：10 July 2019，
  
  Online First：22 July 2019，
  
  Published：2020-02
- Accepted：
Scan QR Code
Wenxiao CUI, Aijun MA, Zhihui HUANG, et al. Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater[J]. Journal of Oceanology and Limnology, 2020, 38(2): 467-479.
DOI：

Wenxiao CUI, Aijun MA, Zhihui HUANG, et al. Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater[J]. Journal of Oceanology and Limnology, 2020, 38(2): 467-479. DOI： 10.1007/s00343-019-9056-2.

摘要

Abstract

Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory plasticity. The kidney transcriptome of turbot treated 24 h in water of hypo-salinity (salinity 5) and seawater (salinity 30) was sequenced and characterized. In silico analysis indicated that all unigenes had significant hits in seven databases. The functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion

absorption

and metabolism played an important role in the osmoregulation of turbot in response to hypo-salinity. Analysis of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic plasticity. Moreover

we analyzed and predicted the mechanisms of canonical signaling transduction. Biological processes involved in renin secretion

ECM-receptor interaction

adherens junction

and focal adhesion played an important role in the plasticity phenotype in hypo-stress

while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse

worked in hypoosmoregulation signal transduction in the turbot. In addition

analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried out. The results showed that the kidney

gill

and spleen were vital regulating organs of osmotic pressure

and the osmoregulation pattern of euryhaline fish differed among species.

关键词

Keywords

references

F A Alves-Costa , E M Denovan-Wright , C Thisse , B Thisse , J M Wright . Spatio-temporal distribution of fatty acid-binding protein 6 ( fabp6 ) gene transcripts in the developing and adult zebrafish ( Danio rerio ) . FEBS J. , 2008 . 275 ( 13 ): 3 325 - 3 334 . DOI: 10.1111/j.1742-4658.2008.06480.x http://doi.org/10.1111/j.1742-4658.2008.06480.x .

S Anders , W Huber . Differential expression analysis for sequence count data . Genome Biol. , 2010 . 11 ( 10 ): R106 DOI: 10.1186/gb-2010-11-10-r106 http://doi.org/10.1186/gb-2010-11-10-r106 .

K C Arden . FoxO:linking new signaling pathways . Mol.Cell , 2004 . 14 ( 4 ): 416 - 418 . DOI: 10.1016/S1097-2765(04)00213-8 http://doi.org/10.1016/S1097-2765(04)00213-8 .

M Babey , P Kopp , G L Robertson . Familial forms of diabetes insipidus:clinical and molecular characteristics . Nat. Rev. Endocrinol. , 2011 . 7 ( 12 ): 701 - 714 . DOI: 10.1038/nrendo.2011.100 http://doi.org/10.1038/nrendo.2011.100 .

Bakir B, Sezerman O U. 2006. Functional classification of G-protein coupled receptors, based on their specific ligand coupling patterns. In : Rothlauf F, Branke J, Cagnoni S, Costa E, Cotta C, Drechsler R, Lutton E, Machado P, Moore J H, Romero J, Smith G D, Squillero G, Takagi H eds. Applications of Evolutionary Computing. Springer, Berlin, Heidelberg. p.1-12.

K W Beyenbach . Secretory NaCl and volume flow in renal tubules . Am. J. Physiol. , 1986 . 250 ( 5 ): R753 - R763 . http://cn.bing.com/academic/profile?id=503f1ac67121be456ba67b2c79c430f3&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=503f1ac67121be456ba67b2c79c430f3&encoded=0&v=paper_preview&mkt=zh-cn , .

K W Beyenbach . Kidneys sans glomeruli . Am. J. Physiol.Renal Physiol. , 2004 . 286 ( 5 ): F811 - F827 . DOI: 10.1152/ajprenal.00351.2003 http://doi.org/10.1152/ajprenal.00351.2003 .

S Bhattacharya . Handbook of cell signaling . J. Anat. , 2004 . 205 ( 1 ): 77 DOI: 10.1111/j.0021-8782.2004.00308.x http://doi.org/10.1111/j.0021-8782.2004.00308.x .

A Bonni , Y Sun , M Nadal-Vicens , A Bhatt , D A Frank , I Rozovsky , N Stahl , G D Yancopoulos , M E Greenberg . Regulation of gliogenesis in the central nervous system by the JAK-STAT signaling pathway . Science , 1997 . 278 ( 5337 ): 477 - 483 . DOI: 10.1126/science.278.5337.477 http://doi.org/10.1126/science.278.5337.477 .

R S Brennan , F Galvez , A Whitehead . Reciprocal osmotic challenges reveal mechanisms of divergence in phenotypic plasticity in the killifish Fundulus heteroclitus . J. Exp. Biol. , 2015 . 218 ( 8 ): 1 212 - 1 222 . DOI: 10.1242/jeb.110445 http://doi.org/10.1242/jeb.110445 .

C P Cutler , G Cramb . Branchial expression of an aquaporin 3 (AQP-3) homologue is downregulated in the European eel Anguilla anguilla following seawater acclimation . J. Exp. Biol. , 2002 . 205 ( 17 ): 2 643 - 2 651 . .

C P Cutler , C Philips , N Hazon , G Cramb . Aquaporin 8(AQP8) intestinal mRNA expression increases in response to salinity acclimation in yellow and silver European eels( Anguilla anguilla ) . Comp. Biochem. Physiol. A Mol.Integr. Physiol. , 2009 . 153 ( S2 ): S78 DOI: 10.1016/j.cbpa.2009.04.047 http://doi.org/10.1016/j.cbpa.2009.04.047 .

C Dietz , K T Stiller , M Griese , C Schulz , A Susenbeth . Influence of salinity on energy metabolism in juvenile turbot, Psetta maxima (L.) . Aquacult. Nutr. , 2013 . 19 ( S1 ): 135 - 150 . DOI: 10.1111/anu.12034 http://doi.org/10.1111/anu.12034 .

S L Edwards , W S Marshall . Principles and patterns of osmoregulation and euryhalinity in fishes . Fish Physiol. , 2012 . 32 1 - 44 . DOI: 10.1016/B978-0-12-396951-4.00001-3 http://doi.org/10.1016/B978-0-12-396951-4.00001-3 .

M Ehebauer , P Hayward , A Martinez-Arias . Notch signaling pathway . Sci. STKE , 2006 . 2006 ( 364 ): m7 DOI: 10.1126/stke.3642006cm7 http://doi.org/10.1126/stke.3642006cm7 .

Farrell A P. 2011. Encyclopedia of Fish Physiology: from Genome to Environment. Academic Press, San Diego.

C J Fielding , V G Shore , P E Fielding . A protein cofactor of lecithin:cholesterol acyltransferase . Biochem. Biophys.Res. Commun. , 1972 . 46 ( 4 ): 1 493 - 1 498 . DOI: 10.1016/0006-291X(72)90776-0 http://doi.org/10.1016/0006-291X(72)90776-0 .

D F Fiol , D Kültz . Osmotic stress sensing and signaling in fishes . FEBS J. , 2007 . 274 ( 22 ): 5 790 - 5 798 . DOI: 10.1111/j.1742-4658.2007.06099.x http://doi.org/10.1111/j.1742-4658.2007.06099.x .

A Fouchs , H Ollivier , C Haond , S Roy , P Calvès , K PichavantRafini . Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes . Biol. Cell , 2010 . 102 ( 8 ): 447 - 456 . DOI: 10.1042/BC20090154 http://doi.org/10.1042/BC20090154 .

S Götz , J M García-Gómez , J Terol , T D Williams , S H Nagaraj , M J Nueda , M Robles , M Talón , J Dopazo , A Conesa . High-throughput functional annotation and data mining with the blast2go suite . Nucleic Acids Res. , 2008 . 36 ( 10 ): 3 420 - 3 435 . DOI: 10.1093/nar/gkn176 http://doi.org/10.1093/nar/gkn176 .

C R Goward , D J Nicholls . Malate dehydrogenase:a model for structure, evolution, and catalysis . Protein Sci. , 1994 . 3 ( 10 ): 1 883 - 1 888 . DOI: 10.1002/pro.5560031027 http://doi.org/10.1002/pro.5560031027 .

M G Grabherr , B J Haas , M Yassour , J Z Levin , D A Thompson , I Amit , X Adiconis , L Fan , R Raychowdhury , Q D Zeng , Z H Chen , E Mauceli , N Hacohen , A Gnirke , N Rhind , F Di Palma , B W Birren , C Nusbaum , K Lindblad-Toh , N Friedman , A Regev . Trinity:reconstructing a fulllength transcriptome without a genome from RNA-Seq data . Nat. Biotechnol. , 2011 . 29 ( 7 ): 644 - 652 . DOI: 10.1038/nbt.1883 http://doi.org/10.1038/nbt.1883 .

S Grant , L Qiao , P Dent . Roles of ERBB family receptor tyrosine kinases, and downstream signaling pathways, in the control of cell growth and survival . Front Biosci. , 2002 . 7 d376 - 389 . http://cn.bing.com/academic/profile?id=418b47fcf4e8cd0ca5446933237eb1a5&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=418b47fcf4e8cd0ca5446933237eb1a5&encoded=0&v=paper_preview&mkt=zh-cn , .

E K Hoffmann , I H Lambert , S F Pedersen . Physiology of cell volume regulation in vertebrates . Physiol. Rev. , 2009 . 89 ( 1 ): 193 - 277 . DOI: 10.1152/physrev.00037.2007 http://doi.org/10.1152/physrev.00037.2007 .

C S Hummel , C Lu , D D F Loo , B A Hirayama , A A Voss , E M Wright . Glucose transport by human renal Na + / D -glucose cotransporters SGLT1 and SGLT2 . Am. J.Physiol. Cell Physiol. , 2011 . 300 ( 1 ): C14 - C21 . DOI: 10.1152/ajpcell.00388.2010 http://doi.org/10.1152/ajpcell.00388.2010 .

A K Imsland , A Foss , S Gunnarsson , M H G Berntssen , R FitzGerald , S W Bonga , Ham E v , G Nævdal , S O Stefansson . The interaction of temperature and salinity on growth and food conversion in juvenile turbot( Scophthalmus maximus ) . Aquaculture , 2001 . 198 ( 3-4 ): 353 - 367 . DOI: 10.1016/S0044-8486(01)00507-5 http://doi.org/10.1016/S0044-8486(01)00507-5 .

M Inokuchi , J Hiroi , S Watanabe , K M Lee , T Kaneko . Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of mozambique tilapia ( Oreochromis mossambicus )acclimated to a wide range of salinities . Comp. Biochem.Physiol. A Mol. Integr. Physiol. , 2008 . 151 ( 2 ): 151 - 158 . DOI: 10.1016/j.cbpa.2008.06.012 http://doi.org/10.1016/j.cbpa.2008.06.012 .

S Kalujnaia , I S McWilliam , V A Zaguinaiko , A L Feilen , J Nicholson , N Hazon , C P Cutler , G Cramb . Transcriptomic approach to the study of osmoregulation in the European eel Anguilla anguilla . Physiol. Genomics , 2007 . 31 ( 3 ): 385 - 401 . DOI: 10.1152/physiolgenomics.00059.2007 http://doi.org/10.1152/physiolgenomics.00059.2007 .

D Karaica , D Breljak , H Brzica , J Lončar , C M Herak-Kramberger , V Micek , I Vrhovac , D J Ivković , I Mihaljević , P Marić , T Smital , B C Burckhardt , G Burckhardt , I Sabolić . CFEX (slc26a6) in rat kidneys, liver, and small intestine in an experimental model of oxalate nephrolitiasis . Arch. Ind. Hyg. Toxicol. , 2015 . 66 ( 3 ): 228 .

Kasprowicz A. 2011. Osmosensing. In: Wojtaszek P ed.Mechanical Integration of Plant Cells and Plants. Springer, Berlin, Heidelberg. p.225-240.

D Kültz , K Avila . Mitogen-activated protein kinases are in vivo transducers of osmosensory signals in fish gill cells . Comp. Biochem. Physiol. B Biochem. Mol. Biol. , 2001 . 129 ( 4 ): 821 - 829 . DOI: 10.1016/S1096-4959(01)00395-5 http://doi.org/10.1016/S1096-4959(01)00395-5 .

D Kültz , M Burg . Evolution of osmotic stress signaling via MAP kinase cascades . J. Exp. Biol. , 1998 . 201 ( 22 ): 3 015 - 3 021 . http://cn.bing.com/academic/profile?id=9139d663dbc1b39ba222f4d5a4209346&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=9139d663dbc1b39ba222f4d5a4209346&encoded=0&v=paper_preview&mkt=zh-cn , .

D Kültz . Physiological mechanisms used by fish to cope with salinity stress . J. Exp. Biol. , 2015 . 218 ( 12 ): 1 907 - 1 914 . DOI: 10.1242/jeb.118695 http://doi.org/10.1242/jeb.118695 .

B Kurt , L Kurtz , M L Sequeira-Lopez , R A Gomez , K Willecke , C Wagner , A Kurtz . Reciprocal expression of connexin 40 and 45 during phenotypical changes in reninsecreting cells . Am. J. Physiol. Renal Physiol. , 2011 . 300 ( 3 ): F743 - F748 . DOI: 10.1152/ajprenal.00647.2010 http://doi.org/10.1152/ajprenal.00647.2010 .

A Kurtz , C Wagner . Cellular control of renin secretion . J. Exp. Biol. , 1999 . 202 ( 3 ): 219 - 225 . http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_185759 http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_185759 , .

S H Lam , E Y Lui , Z J Li , S J Cai , W K Sung , S Mathavan , T J Lam , Y K Ip . Differential transcriptomic analyses revealed genes and signaling pathways involved in ionoosmoregulation and cellular remodeling in the gills of euryhaline mozambique tilapia, Oreochromis mossambicus . BMC Genomics , 2014 . 15 ( 1 ): 921 DOI: 10.1186/1471-2164-15-921 http://doi.org/10.1186/1471-2164-15-921 .

K M Lee , T Kaneko , F Katoh , K Aida . Prolactin gene expression and gill chloride cell activity in fugu Takifugu rubripes exposed to a hypoosmotic environment . Gen.Comp. Endocrinol. , 2006 . 149 ( 3 ): 285 - 293 . DOI: 10.1016/j.ygcen.2006.06.009 http://doi.org/10.1016/j.ygcen.2006.06.009 .

B Li , C N Dewey . Rsem:accurate transcript quantification from RNA-Seq data with or without a reference genome . BMC Bioinformatics , 2011 . 12 323 DOI: 10.1186/1471-2105-12-323 http://doi.org/10.1186/1471-2105-12-323 .

X Z Mao , T Cai , J G Olyarchuk , L P Wei . Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary . Bioinformatics , 2005 . 21 ( 19 ): 3 787 - 3 793 . DOI: 10.1093/bioinformatics/bti430 http://doi.org/10.1093/bioinformatics/bti430 .

Marshall W, Grosell M. 2005. Ion transport, osmoregulation, and acid-base balance. In : Evans D H, Claiborne J B eds.The Physiology of Fishes. 3 rd ed. CRC Press, Boca Raton.p.177-230.

B Y K Narnaware , S P Kelly , N Y S Woo . Effect of salinity and ration size on macrophage phagocytosis in juvenile black sea bream ( Mylio macrocephalus ) . J. Appl.Ichthyol. , 2000 . 16 ( 2 ): 86 - 88 . DOI: 10.1046/j.1439-0426.2000.00113.x http://doi.org/10.1046/j.1439-0426.2000.00113.x .

G Nishimoto , G Sasaki , E Yaoita , M Nameta , H Li , K Furuse , H Fujinaka , Y Yoshida , A Mitsudome , T Yamamoto . Molecular characterization of water-selective AQP (EbAQP4)in hagfish:insight into ancestral origin of AQP4 . Am. J.Physiol. Regul. Integr. Comp. Physiol. , 2007 . 292 ( 1 ): R644 - R651 . DOI: 10.1152/ajpregu.00362.2006 http://doi.org/10.1152/ajpregu.00362.2006 .

P Oelkers , L C Kirby , J E Heubi , P A Dawson . Primary bile acid malabsorption caused by mutations in the ileal sodium-dependent bile acid transporter gene (SLC10A2) . J. Clin. Invest. , 1997 . 99 ( 8 ): 1 880 - 1 887 . DOI: 10.1172/JCI119355 http://doi.org/10.1172/JCI119355 .

P Pereiro , P Balseiro , A Romero , S Dios , G Forn-Cuni , B Fuste , J V Planas , S Beltran , B Novoa , A Figueras . Highthroughput sequence analysis of turbot ( Scophthalmus maximus ) transcriptome using 454-pyrosequencing for the discovery of antiviral immune genes . PLoS One , 2012 . 7 ( 5 ): e35369 DOI: 10.1371/journal.pone.0035369 http://doi.org/10.1371/journal.pone.0035369 .

G E Pickford , J G Phillips . Prolactin, a factor in promoting survival of hypophysectomized killifish in fresh water . Science , 1959 . 130 ( 3373 ): 454 - 455 . DOI: 10.1126/science.130.3373.454 http://doi.org/10.1126/science.130.3373.454 .

Prodocimo , V , C F Souza , C Pessini , L C Fernandes , C A Freire . Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction . Neotrop. Ichthyol. , 2008 . 6 ( 4 ): 613 - 620 . DOI: 10.1590/S1679-62252008000400009 http://doi.org/10.1590/S1679-62252008000400009 .

Rappolee D A, Armant D R. 2009. Cell signaling. In : Krawetz S ed. Bioinformatics for Systems Biology. Humana Press, New York. p.89-104.

T D Schmittgen , B A Zakrajsek . Effect of experimental treatment on housekeeping gene expression:validation by real-time, quantitative RT-PCR . J. Biochem. Biophys.Methods , 2000 . 46 ( 1-2 ): 69 - 81 . DOI: 10.1016/S0165-022X(00)00129-9 http://doi.org/10.1016/S0165-022X(00)00129-9 .

Y Shibata , M Kumai , K Nishii , K Nakamura . Diversity and molecular anatomy of gap junctions . Med. Electron Microsc. , 2001 . 34 ( 3 ): 153 - 159 . DOI: 10.1007/s007950100008 http://doi.org/10.1007/s007950100008 .

X M Tang , Z Sui , J B Tian , G F Wang . Effects of salinity on metabolic rate of juvenile turbot ( Scophamus maximus ) . South China Fish. Sci. , 2006 . 2 ( 4 ): 54 - 58 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=nfsc200604009 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=nfsc200604009 , .

C Trapnell , B A Williams , G Pertea , A Mortazavi , G Kwan , M J Van Baren , S L Salzberg , B J Wold , L Pachter . Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation . Nat. Biotechnol , 2010 . 28 ( 5 ): 511 - 515 . DOI: 10.1038/nbt.1621 http://doi.org/10.1038/nbt.1621 .

A B Vojtek , C J Der . Increasing complexity of the Ras signaling pathway . J. Biol. Chem. , 1998 . 273 ( 32 ): 19 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1ed2bca9f91f6c6d1e7b0bfc368626c5 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1ed2bca9f91f6c6d1e7b0bfc368626c5 , .

Z Z Xu , L Gan , T Y Li , C Xu , K Chen , X D Wang , J G Qin , L Q Chen , E C Li . Transcriptome profiling and molecular pathway analysis of genes in association with salinity adaptation in Nile tilapia Oreochromis niloticus . PLoS One , 2015 . 10 ( 8 ): e0136506 DOI: 10.1371/journal.pone.0136506 http://doi.org/10.1371/journal.pone.0136506 .

M D Young , M J Wakefield , G K Smyth , A Oshlack . Gene ontology analysis for RNA-seq:accounting for selection bias . Genome Biol. , 2010 . 11 ( 2 ): R14 DOI: 10.1186/gb-2010-11-2-r14 http://doi.org/10.1186/gb-2010-11-2-r14 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency

Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus

Estimation of genetic parameters for upper thermal tolerances and growth-related traits in turbot Scophthalmus maximus

Related Author

Yuting SONG

Maqsood Ahmed SOOMRO

Xianzhi DONG

Guobin HU

Junqiong SHI

Shuhan HE

Lu ZHAO

Lulu JI

Related Institution

Institute of Biophysis, Chinese Academy of Sciences

Institute of Evolution & Marine Biodiversity, Ocean University of China

College of Marine Life Sciences, Ocean University of China

Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University

Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao)

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

DOI：10.1007/s00343-019-9056-2

摘要

Abstract

关键词

Keywords

references