Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences

Kaihui LU; Weiwei LIU; WARREN Alan; Yusen XU; Changyu ZHU; Yan ZHAO; Zhenzhen YI

doi:10.1007/s00343-019-9021-0

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Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences

Ecology | Updated：2023-04-27

- Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences
- Journal of Oceanology and Limnology Vol. 38, Issue 1, Pages: 156-167(2020)
- Affiliations：
  
  1.Laboratory of Protozoology, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life Science, South China Normal University, Guangzhou 510631, China
  2.Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
  3.Department of Life Sciences, Natural History Museum, London SW7 5BD, United K ingdom
  4.College of Life Sciences, Capital Normal University, Beijing 100048, China
- Author bio：
  
  YI Zhenzhen, zyi@scnu.edu.cn
- Funds：
- DOI：10.1007/s00343-019-9021-0
  CLC：
- Received：24 January 2019，
  
  Accepted：18 March 2019，
  
  Online First：25 April 2019，
  
  Published：2020-01
- Accepted：
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Kaihui LU, Weiwei LIU, WARREN Alan, et al. Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences[J]. Journal of Oceanology and Limnology, 2020, 38(1): 156-167.
DOI：

Kaihui LU, Weiwei LIU, WARREN Alan, et al. Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences[J]. Journal of Oceanology and Limnology, 2020, 38(1): 156-167. DOI： 10.1007/s00343-019-9021-0.

摘要

Abstract

Although the taxonomy of oligotrich ciliates has been widely investigated

yet the species diversity remains poorly known. We newly designed a pair of oligotrich-specific LSU rDNA primers covering the 600-bp D1/D2 region

and it was effective for detecting oligotrich species. Using the primers

we constructed the cloning libraries to investigate the species diversity of oligotrichs in the northern coastal waters of the South China Sea. In total

165 oligotrich sequences were obtained from five widely separated sampling sites. Sixty operational taxonomic units (OTUs) were obtained at 99% similarity threshold

and low-abundance OTUs with no more than two sequences contributed most of these (about 78%). Our findings are consistent with previous morphological studies

Strombidium

was found the most abundant and widely distributed genus in this area. In addition

the BLAST search in the NCBI database resulted in 95% OTUs matching with named oligotrich species in similarity below 99%. Therefore

oligotrich morphospecies diversity has been underestimated as low-abundance species

and the LSU rDNA oligotrich sequence database needs to be better promoted.

关键词

Keywords

references

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