Transcriptional responses to starvation of pathogenic <italic style="font-style: italic">Vibrio harveyi</italic> strain DY1

Xiaodan LIU; Xiaojian GAO; Nan CHEN; Yingying ZHANG; Xixi LI; Yue ZHANG; Xiaojun ZHANG

doi:10.1007/s00343-019-8350-3

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Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1

Aquaculture and Fisheries | Updated：2023-04-27

- Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1
- Journal of Oceanology and Limnology Vol. 38, Issue 2, Pages: 579-587(2020)
- Affiliations：
  
  College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
- Author bio：
  
  ZHANG Xiaojun, zxj9307@163.com
- Funds：
- DOI：10.1007/s00343-019-8350-3
  CLC：
- Received：04 January 2019，
  
  Accepted：28 April 2019，
  
  Online First：16 July 2019，
  
  Published：2020-02
- Accepted：
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Xiaodan LIU, Xiaojian GAO, Nan CHEN, et al. Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1[J]. Journal of Oceanology and Limnology, 2020, 38(2): 579-587.
DOI：

Xiaodan LIU, Xiaojian GAO, Nan CHEN, et al. Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1[J]. Journal of Oceanology and Limnology, 2020, 38(2): 579-587. DOI： 10.1007/s00343-019-8350-3.

摘要

Abstract

Vibrio harveyi

is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry. Recurrent outbreaks of vibriosis are closely correlated with the capacity of this bacterial species to survive long-term starvation conditions. To study the regulation mechanism of gene expression at the transcriptional level in

V. harveyi

under starvation conditions

the transcriptomic response profiles were determined of the Portunus trituberculatus pathogen

V. harveyi

strain DY1 under normal conditions and after four weeks of starvation. A total of 4 679 and 4 661 genes were expressed in the non-starved and starved cells

respectively. The significantly differentially expressed genes (DEGs) between non-starved and starved groups were identified

in which 255 genes were up-regulated and 411 genes were down-regulated. GO analysis and KEGG enrichment analysis were used to analyze the DEGs and revealed the involvement of these DEGs in many pathways

including ABC transporters

flagellum assembly

and fatty acid metabolism. Several DEGs were randomly selected and their expression levels were confirmed by quantitative real-time PCR (qRT-PCR). This is the first comprehensive transcriptomic analysis of starvation effects in

V. harveyi

. Our findings will facilitate future study on stress adaptation and survival mechanisms of

V. harveyi

关键词

Keywords

references

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Related Institution

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Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1

DOI：10.1007/s00343-019-8350-3

摘要

Abstract

关键词

Keywords

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