Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in <italic style="font-style: italic">Chlamydomonas reinhardtii</italic>

MIAO Rongli; HUANG Kaiyao

doi:10.1007/s00343-019-8345-0

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Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii

Updated：2023-04-27

- Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii
- Journal of Oceanology and Limnology Vol. 38, Issue 2, Pages: 517-528(2020)
- Affiliations：
  
  1.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  MIAO Rongli, mronli@ihb.ac.cn
- Funds：
- DOI：10.1007/s00343-019-8345-0
  CLC：
- Received：30 November 2018，
  
  Accepted：10 May 2019，
  
  Online First：16 July 2019，
  
  Published：2020-03
- Accepted：
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MIAO Rongli, HUANG Kaiyao. Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii[J]. Journal of Oceanology and Limnology, 2020, 38(2): 517-528.
DOI：

MIAO Rongli, HUANG Kaiyao. Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii[J]. Journal of Oceanology and Limnology, 2020, 38(2): 517-528. DOI： 10.1007/s00343-019-8345-0.

摘要

Abstract

The unicellular green alga

Chlamydomonas reinhardtii

is a model organism for studying various biological processes

such as photosynthesis

flagellar motility

and lipid metabolism. To find some novel genes regulating the lipid metabolism under various stress conditions

the paromomycin resistance gene

aphVⅢ

was transferred into the genome of

C. reinhardtii

to establish a mutant library. Two genes mutated in two of the TAG-reduced mutants (Cre06.g278111 in M2 mutant

Cre06.g278110 in M6 mutants) were neighboring in the genome

and their expression levels were down-regulated in their corresponding mutants in parallel with their reduced TAG levels following N deprivation. The proteins encoded by these two genes (KCN11 by Cre06.g278111

ACYC3 by Cre06.g278110) contained a conversed cyclic mononucleotide phosphate (cNMP) binding protein and an adenylate domain

respectively. Since cNMP binding protein and adenylate domain have been known as important components of cyclic adenosine monophosphate (cAMP) signaling pathway

suggesting that these two genes might affect cellular TAG biosynthesis through cAMP signal pathway.

关键词

Keywords

references

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Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii

DOI：10.1007/s00343-019-8345-0

摘要

Abstract

关键词

Keywords

references