- About us
- Contents
- For Authors
- For Reviewers
- Ethics
- Personal Center
- Logout
FOLLOWUS
- About us
- Overview
- Aims & Scope
- Editorial Board
- Subscribe
- Contents
- Current Issue
- Archive
- Online First
- Special Issues
- Virtual Issues
- Videos
- For Authors
- Submit
- Submission Guidelines
- Journal Data Policy
- Fees & Funding
- Downloads
- For Reviewers
- Reviewer Guidelines
- Submit Your Review Report
- Ethics
- Introduction
- Editor Responsibilities
- Reviewer Responsibilit
- Author Responsibilities
- Publisher’s Confirmation
Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium,
Halomonas sp. YSR-3- Journal of Oceanology and Limnology Vol. 38, Issue 1, Pages: 195-203(2020)
- Affiliations：
1.Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huai'an 223003, China
2.Jiangsu Provincial Engineering Laboratory for Advanced Materials of Salt Chemical Industry, Huaiyin Institute of Technology, Huai'an 223003, China
3.School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China
- Author bio：
LUO Chuping, luochuping@163.com
LIU Fei, hyitliu@163.com
- Funds：
DOI：10.1007/s00343-019-8323-6
CLC：Received：06 November 2018，
Accepted：24 December 2018，
Online First：08 March 2019，
Published：2020-01
- Accepted：
Scan QR Code
Shiying REN, Xiangqian LI, Xiulian YIN, et al. Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium,
Halomonas sp. YSR-3[J]. Journal of Oceanology and Limnology, 2020, 38(1): 195-203.Shiying REN, Xiangqian LI, Xiulian YIN, et al. Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium,
Halomonas sp. YSR-3[J]. Journal of Oceanology and Limnology, 2020, 38(1): 195-203. DOI： 10.1007/s00343-019-8323-6.
摘要
Abstract
Halomonas
sp. YSR-3 was isolated from the Yellow Sea and identified as a polyphosphateaccumulating bacterium and the characteristics of its intracellular polyphosphate (polyP) granules and phosphorus absorption were studied. Most YSR-3 cells stored one or two polyP granules in regular appearance and high-density. The diameter of the granules was about 400 nm measuring by a transmission electron microscope (TEM). After stained with 4
6-diamidino-2-phenylindole (DAPI) and visualized by a fluorescence microscope
the cells turned blue and the granules were bright yellow. The composition of granules includes P (major ingredient)
Mg
S
K
and Ca as detected by an energy dispersive X-ray spectrometer (EDS). When inorganic phosphorus (PO
4
3-
) and ferric ion (Fe
3+
) were added into media
the biomass increased and the cells formed intracellular polyP granules owing to the phosphorus assimilation from media. The YSR-3 obtained higher biomass by adding 0.02 g/L FePO
4
than 0.005 g/L and 0.01 g/L FePO
4
; however
the phosphorus absorption was higher with 0.01 g/L FePO
4
than 0.005 g/L and 0.02 g/L FePO
4
. The optical density at wavelength 480 nm (OD
480 nm
) was 0.79 and 100% cells could form intracellular polyP granules. These results show that strain YSR-3 is able to acquire higher biomass and absorb more inorganic phosphorus when 0.01 g/L FePO
4
is added. The characteristics of absorbing and storing phosphorus as intracellular inorganic polyP granules have a potential for application in high-efficiency phosphorus removal in wastewater treatment.
关键词
Keywords
references
A Akar , E U Akkaya , S K Yesiladali , G Çelikyilmaz , E U Çokgor , C Tamerler , D Orhon , Z P Çakar . Accumulation of polyhydroxyalkanoates by Microlunatus phosphovorus under various growth conditions . Journal of Industrial Microbiology and Biotechnology , 2006 . 33 ( 3 ): 215 - 220 . DOI: 10.1007/s10295-004-0201-2 http://doi.org/10.1007/s10295-004-0201-2 .
C Alcántara , A Blasco , M Zúñiga , V Monedero . Accumulation of polyphosphate in Lactobacillus spp. and its involvement in stress resistance . Applied and Environmental Microbiology , 2014 . 80 ( 5 ): 1 650 - 1 659 . DOI: 10.1128/AEM.03997-13 http://doi.org/10.1128/AEM.03997-13 .
S Bru , J M Martínez-Laínez , S Hernández-Ortega , E Quandt , J Torres-Torronteras , R Martí , D Canadell , J Ariño , S Sharma , J Jiménez , J Clotet . Polyphosphate is involved in cell cycle progression and genomic stability in Saccharomyces cerevisiae . Molecular Microbiology , 2016 . 101 ( 3 ): 367 - 380 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1111/mmi.13396 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1111/mmi.13396 , .
W J Cai , X P Hu , W J Huang , M C Murrell , J C Lehrter , S E Lohrenz , W C Chou , W D Zhai , J T Hollibaugh , Y C Wang , P S Zhao , X H Guo , K Gundersen , M H Dai , G C Gong . Acidification of subsurface coastal waters enhanced by eutrophication . Nature Geoscience , 2011 . 4 ( 11 ): 766 - 770 . DOI: 10.1038/ngeo1297 http://doi.org/10.1038/ngeo1297 .
V Chaudhry , C S Nautiyal . A high throughput method and culture medium for rapid screening of phosphate accumulating microorganisms . Bioresource Technology , 2011 . 102 ( 17 ): 8 057 - 8 062 . DOI: 10.1016/j.biortech.2011.05.045 http://doi.org/10.1016/j.biortech.2011.05.045 .
M J Church , D A Hutchins , H W Ducklow . Limitation of bacterial growth by dissolved organic matter and iron in the southern ocean . Applied and Environmental Microbiology , 2000 . 66 ( 2 ): 455 - 466 . DOI: 10.1128/AEM.66.2.455-466.2000 http://doi.org/10.1128/AEM.66.2.455-466.2000 .
H J Du , R Zhang , W Y Zhang , C Xu , Y R Chen , H M Pan , K Zhou , L F Wu , T Xiao . Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China . Acta Oceanologica Sinica , 2017 . 36 ( 2 ): 94 - 104 . DOI: 10.1007/s13131-017-0980-8 http://doi.org/10.1007/s13131-017-0980-8 .
H M El-Kamah , S A Badr , R M Moghazy . Reuse of wastewater treated effluent by lagoon for agriculture and aquaculture purposes . Australian Journal of Basic and Applied Sciences , 2011 . 5 ( 10 ): 9 - 17 . .
O Gillor , O Hadas , A F Post , S Belkin . Phosphorus and nitrogen in a monomictic freshwater lake:employing cyanobacterial bioreporters to gain new insights into nutrient bioavailability . Freshwater Biology , 2010 . 55 ( 6 ): 1 182 - 1 190 . DOI: 10.1111/j.1365-2427.2009.02342.x http://doi.org/10.1111/j.1365-2427.2009.02342.x .
J P Harrison , J E Hallsworth , C S Cockell . Reduction of the temperature sensitivity of Halomonas hydrothermalis by iron starvation combined with microaerobic conditions . Applied and Environmental Microbiology , 2015 . 81 ( 6 ): 2 156 - 2 162 . DOI: 10.1128/AEM.03639-14 http://doi.org/10.1128/AEM.03639-14 .
J T Henry , S Crosson . Chromosome replication and segregation govern the biogenesis and inheritance of inorganic polyphosphate granules . Molecular Biology of the Cell , 2013 . 24 ( 20 ): 3 177 - 3 186 . DOI: 10.1091/mbc.e13-04-0182 http://doi.org/10.1091/mbc.e13-04-0182 .
R Hirota , A Kuroda , J Kato , H Ohtake . Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses . Journal of Bioscience and Bioengineering , 2010 . 109 ( 5 ): 423 - 432 . DOI: 10.1016/j.jbiosc.2009.10.018 http://doi.org/10.1016/j.jbiosc.2009.10.018 .
Z Y Luan , H B Qi , D H Li . The experimental study of using calcium silicate slag to recover phosphorus in polluted water . Advanced Materials Research , 2013 . 610-613 1 595 - 1 600 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.4028/www.scientific.net/AMR.610-613.1595 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.4028/www.scientific.net/AMR.610-613.1595 , .
R Lucas , C Courties , A Herbland , P Goulletquer , A L Marteau , H Lemonnier . Eutrophication in a tropical pond:understanding the bacterioplankton and phytoplankton dynamics during a vibriosis outbreak using flow cytometric analyses . Aquaculture , 2010 . 310 ( 1-2 ): 112 - 121 . DOI: 10.1016/j.aquaculture.2010.10.022 http://doi.org/10.1016/j.aquaculture.2010.10.022 .
P Martin , S T Dyhrman , M W Lomas , N J Poulton , B A S Van Mooy . Accumulation and enhanced cycling of polyphosphate by Sargasso Sea plankton in response to low phosphorus . Proceedings of the National Academy of Sciences of the United States of America , 2014 . 111 ( 22 ): 8 089 - 8 094 . DOI: 10.1073/pnas.1321719111 http://doi.org/10.1073/pnas.1321719111 .
M Monachese , J P Burton , G Reid . Bioremediation and tolerance of humans to heavy metals through microbial processes:a potential role for probiotics? . Applied and Environmental Microbiology , 2012 . 78 ( 18 ): 6 397 - 6 404 . DOI: 10.1128/AEM.01665-12 http://doi.org/10.1128/AEM.01665-12 .
A Muszyński , A Miłobędzka . The effects of carbon/phosphorus ratio on polyphosphate-and glycogenaccumulating organisms in aerobic granular sludge . International Journal of Environmental Science and Technology , 2015 . 12 ( 9 ): 3 053 - 3 060 . DOI: 10.1007/s13762-015-0828-8 http://doi.org/10.1007/s13762-015-0828-8 .
K Nakamura , A Hiraishi , Y Yoshimi , M Kawaharasaki , K Masuda , Y Kamagata . Microlunatus phosphovorus gen.nov., sp. nov., a new gram-positive polyphosphateaccumulating bacterium isolated from activated sludge . International Journal of Systematic Bacteriology , 1995 . 45 ( 1 ): 17 - 22 . DOI: 10.1099/00207713-45-1-17 http://doi.org/10.1099/00207713-45-1-17 .
Y H Ong , A S M Chua , T Fukushima , G C Ngoh , T Shoji , A Michinaka . High-temperature EBPR process:the performance, analysis of PAOs and GAOs and the finescale population study of Candidatus Accumulibacter phosphatis . Water Research , 2014 . 64 102 - 112 . DOI: 10.1016/j.watres.2014.06.038 http://doi.org/10.1016/j.watres.2014.06.038 .
S Y Ren , Y H Zhang , W C Zhang , T Xiao . Phosphateabsorbing characterization of a marine polyphosphateaccumulating bacterium Halomonas YSR-3 . Chinese High Technology Letters , 2008 . 18 ( 7 ): 743 - 747 . .
M Saleem , A Bukhari , M N Akram . Electrocoagulation for the treatment of wastewater for reuse in irrigation and plantation . Journal of Basic and Applied Sciences , 2011 . 7 ( 1 ): 11 - 20 . .
M J Seufferheld , H M Alvarez , M E Farias . Role of polyphosphates in microbial adaptation to extreme environments . Applied and Environmental Microbiology , 2008 . 74 ( 19 ): 5 867 - 5 874 . DOI: 10.1128/AEM.00501-08 http://doi.org/10.1128/AEM.00501-08 .
W D Tian , C M Lopez-Vazquez , W G Li , D Brdjanovic , M C M van Loosdrecht . Occurrence of PAOI in a low temperature EBPR system . Chemosphere , 2013 . 92 ( 10 ): 1 314 - 1 320 . DOI: 10.1016/j.chemosphere.2013.05.009 http://doi.org/10.1016/j.chemosphere.2013.05.009 .
S J Van Dien , J D Keasling . Control of polyphosphate metabolism in genetically engineered Escherichia coli . Enzyme and Microbial Technology , 1999 . 24 ( 1-2 ): 21 - 25 . DOI: 10.1016/S0141-0229(98)00083-0 http://doi.org/10.1016/S0141-0229(98)00083-0 .
D G Weissbrodt , G S Schneiter , J M Fürbringer , C Holliger . Identification of trigger factors selecting for polyphosphate-and glycogen-accumulating organisms in aerobic granular sludge sequencing batch reactors . Water Research , 2013 . 47 ( 19 ): 7 006 - 7 018 . DOI: 10.1016/j.watres.2013.08.043 http://doi.org/10.1016/j.watres.2013.08.043 .
E A Wolin , M J Wolin , R S Wolfe . Formation of methane by bacterial extracts . The Journal of Biological Chemistry , 1963 . 238 2 882 - 2 886 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1177/088532829100500404 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1177/088532829100500404 , .
0
Views
0
Downloads
0
CSCD
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
- Tel：+86-532-82898754 Email：jol@qdio.ac.cn
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
