Isolation, characterization and expression analysis of <italic style="font-style: italic">TRPV4</italic> in half-smooth tongue sole <italic style="font-style: italic">Cynoglossus semilaevis</italic>

Xiaomei SHANG; Aijun MA; Xin'an WANG; Dandan XIA; Jiao ZHUANG

doi:10.1007/s00343-019-8316-5

Your Location：

Home >

Browse articles >

Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

Aquaculture and Fisheries | Updated：2023-04-27

- Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis
- Journal of Oceanology and Limnology Vol. 38, Issue 1, Pages: 294-305(2020)
- Affiliations：
  
  1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  3.College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
- Author bio：
  
  MA Aijun, maaj@ysfri.ac.cn
- Funds：
- DOI：10.1007/s00343-019-8316-5
  CLC：
- Received：06 November 2018，
  
  Accepted：26 February 2019，
  
  Online First：22 March 2019，
  
  Published：2020-01
- Accepted：
Scan QR Code
Xiaomei SHANG, Aijun MA, Xin'an WANG, et al. Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis[J]. Journal of Oceanology and Limnology, 2020, 38(1): 294-305.
DOI：

Xiaomei SHANG, Aijun MA, Xin'an WANG, et al. Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis[J]. Journal of Oceanology and Limnology, 2020, 38(1): 294-305. DOI： 10.1007/s00343-019-8316-5.

摘要

Abstract

The transient receptor potential vanilloid 4 (TRPV4)

another Ca

entry channel

belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals. However

our understanding of the TRPV4 in fish remains poor. Therefore

we studied the

TRPV4

gene from

Cynoglossus semilaevis

named

CsTRPV4

that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4

including the domains of ANK repeats

six TM

TRP domain

and CaMBD. The

CsTRPV4

was expressed ubiquitously in examined tissues:higher expression in the heart

spleen

testis

and eye

but lower expression in kidney and liver. Surprisingly

the expression of

CsTRPV4

in lateral line was significantly higher than in many other tissues as the

CsTRPV4

was expressed significantly in the free neuromasts. In addition

CsTRPV4

in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts. Therefore

the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in

C. semilaevis

which lays a foundation for further study of the functions of the free neuromasts.

关键词

Keywords

references

V Amato , E Viña , M G Calavia , M C Guerrera , R Laurà , M Navarro , F De Carlos , J Cobo , A Germanà , J A Vega . Trpv4 in the sensory organs of adult zebrafish . Microscopy Research & Technique , 2011 . 75 ( 1 ): 89 - 96 . .

M Bossus , G Charmantier , C Lorin-Nebel . Transient receptor potential vanilloid 4 in the European sea bass Dicentrarchuslabrax : a candidate protein for osmosensing . Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , 2011 . 160 ( 1 ): 43 - 51 . .

A G Carton , J C Montgomery . Responses of lateral line receptors to water flow in the Antarcticnotothenioid, Trematomusbernacchii . Polar Biology , 2002 . 25 ( 10 ): 789 - 793 . DOI: 10.1007/s00300-002-0416-5 http://doi.org/10.1007/s00300-002-0416-5 .

M J Caterina , A Leffler , A B Malmberg , W J Martin , J Trafton , K R Petersen-Zeitz , M Koltzenburg , A I Basbaum , D Julius . Impaired nociception and pain sensation in mice lacking the capsaicin receptor . Science , 2000 . 288 ( 5464 ): 306 - 313 . DOI: 10.1126/science.288.5464.306 http://doi.org/10.1126/science.288.5464.306 .

M J Caterina , T A Rosen , M Tominaga , A J Brake , D Julius . A capsaicin-receptor homologue with a high threshold for noxious heat . Nature , 1999 . 398 ( 6726 ): 436 - 441 . DOI: 10.1038/18906 http://doi.org/10.1038/18906 .

D E Clapham . TRP channels as cellular sensors . Nature , 2003 . 426 ( 6966 ): 517 - 524 . DOI: 10.1038/nature02196 http://doi.org/10.1038/nature02196 .

H A Colbert , T L Smith , C I Bargmann . OSM-9, a novel protein with structural similarity to channels, is required for olfaction, mechanosensation, and olfactory adaptation in Caenorhabditis elegans . The Journal of Neuroscience , 1997 . 17 ( 21 ): 8 259 - 8 269 . DOI: 10.1523/JNEUROSCI.17-21-08259.1997 http://doi.org/10.1523/JNEUROSCI.17-21-08259.1997 .

M P Cuajungco , C Grimm , K Oshima , D D'hoedt , B Nilius , AR Mensenkamp , RJ M Bindels , M Plomann , S Heller . PACSINs bind to the TRPV4 cation channel PACSIN 3 modulates the subcellular localization of TRPV4 . Journal of Biological Chemistry , 2006 . 281 ( 27 ): 18 753 - 18 762 . DOI: 10.1074/jbc.M602452200 http://doi.org/10.1074/jbc.M602452200 .

D D'Hoedt , G Owsianik , J Prenen , MP Cuajungco , C Grimm , S Heller , T Voets , B Nilius . Stimulus-specific modulation of the cation channel TRPV4 by PACSIN 3 . Journal of Biological Chemistry , 2008 . 283 ( 10 ): 6 272 - 6 280 . DOI: 10.1074/jbc.M706386200 http://doi.org/10.1074/jbc.M706386200 .

C Dambly-Chaudière , D Sapède , F Soubiran , K Decorde , N Gompel , A Ghysen . The lateral line of zebrafish: a model system for the analysis of morphogenesis and neural development in vertebrates . Biology of the Cell , 2003 . 95 ( 9 ): 579 - 587 . DOI: 10.1016/j.biolcel.2003.10.005 http://doi.org/10.1016/j.biolcel.2003.10.005 .

S Dijkgraaf . The functioning and significance of the lateral-line organs . Biological Reviews , 1963 . 38 ( 1 ): 51 - 105 . DOI: 10.1111/j.1469-185X.1963.tb00654.x http://doi.org/10.1111/j.1469-185X.1963.tb00654.x .

Eid S R, Cortright D N. 2009. Transient receptor potential channels on sensory nerves. In : Canning B, Spina D eds. Sensory Nerves. Springer, Berlin, Heidelberg.

J Engelmann , W Hanke , J Mogdans , H Bleckmann . Hydrodynamic stimuli and the fish lateral line . Nature , 2000 . 408 ( 6808 ): 51 - 52 . DOI: 10.1038/35040706 http://doi.org/10.1038/35040706 .

W Everaerts , B Nilius , G Owsianik . The vanilloid transient receptor potential channel TRPV4: From structure to disease . Progress in Biophysics and Molecular Biology , 2010 . 103 ( 1 ): 2 - 17 . http://d.old.wanfangdata.com.cn/NSTLQK/10.1016-j.pbiomolbio.2009.10.002/ http://d.old.wanfangdata.com.cn/NSTLQK/10.1016-j.pbiomolbio.2009.10.002/ , .

J Galindo-Villegas , A Montalban-Arques , S Liarte , S De Oliveira , C Pardo-Pastor , F Rubio-Moscardo , J Meseguer , M A Valverde , V Mulero . Correction: cutting edge: trpv4-mediated detection of hyposmotic stress by skin keratinocytes activates developmental immunity . Journal of Immunology , 2016 . 196 ( 8 ): 3 494 DOI: 10.4049/jimmunol.1600243 http://doi.org/10.4049/jimmunol.1600243 .

Garcia-Elias A, Mrkonjić S, Jung C, Pardo-Pastor C, Vicente R, Valverde M A. 2014. The trpv4 channel. In : Nilius B, Flockerzi V eds. Mammalian Transient Receptor Potential (TRP) Cation Channels.Springer, Berlin, Heidelberg.

R Gaudet . A primer on ankyrin repeat function in TRP channels and beyond . Molecular BioSystems , 2008 . 4 ( 5 ): 372 - 379 . DOI: 10.1039/b801481g http://doi.org/10.1039/b801481g .

Kawamura G, Masuma S, Tezuka N, Koiso M, Jinbo T, Namba K. 2003. Morphogenesis of sense organs in the bluefin tuna Thunnus orientalis : The big fish bang. In : Proceedings of the 26th Annual Larval Fish Conference. Norwegian Institute of Marine Research, Bergen, Norway. p.123-135.

H Koyama , R Kishida , R C Goris , T Kusunoki . Organization of the primary projections of the lateral line nerves in the lamprey Lampetra japonica . The Journal of Comparative Neurology , 1990 . 295 ( 2 ): 277 - 289 . DOI: 10.1002/cne.902950210 http://doi.org/10.1002/cne.902950210 .

K Y Kwan , J M Glazer , D P Corey , F L Rice , C L Stucky . TRPA1 modulates mechanotransduction in cutaneous sensory neurons . Journal of Neuroscience , 2009 . 29 ( 15 ): 4 808 - 4 819 . DOI: 10.1523/JNEUROSCI.5380-08.2009 http://doi.org/10.1523/JNEUROSCI.5380-08.2009 .

W Liedtke , Y Choe , M A Martí-Renom , BellAM , C S Denis , A Šali , A J Hudspeth , J M Friedman , S Heller . Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor . Cell , 2000 . 103 ( 3 ): 525 - 535 . DOI: 10.1016/S0092-8674(00)00143-4 http://doi.org/10.1016/S0092-8674(00)00143-4 .

W Liedtke , D M Tobin , C I Bargmann , J M Friedman . Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans . Proceedings of the National Academy of Sciences of the United States of America , 2003 . 100 ( Suppl. 2 ): 14 531 - 14 536 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=035ca5bebb58c2e53f6aa52650611242 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=035ca5bebb58c2e53f6aa52650611242 , .

W Liedtke . Role of TRPV ion channels in sensory transduction of osmotic stimuli in mammals . Experimental Physiology , 2007 . 92 ( 3 ): 507 - 512 . DOI: 10.1113/expphysiol.2006.035642 http://doi.org/10.1113/expphysiol.2006.035642 .

K J Livak , T D Schmittgen . Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔ C t method . Methods , 2001 . 25 ( 4 ): 402 - 408 . DOI: 10.1006/meth.2001.1262 http://doi.org/10.1006/meth.2001.1262 .

A J Ma , X Z Liu , Y J Xu , Y Liang , Z M Zhuang . Feeding rhythm and growth of the tongue sole, Cynoglossus semilaevis Günther, during its early life stages . Aquaculture Research , 2006 . 37 ( 6 ): 586 - 593 . DOI: 10.1111/j.1365-2109.2006.01466.x http://doi.org/10.1111/j.1365-2109.2006.01466.x .

A J Ma , X A Wang , Z M Zhuang , X M Zhang , L J Zhang . Structure of retina and visual characteristics of the half-smooth tongue-sole Cynoglossus semilaevis Günter . Acta Zoologica Sinica , 2007b . 53 ( 2 ): 354 - 363 . .

A J Ma , X A Wang , Z M Zhuang . Lateral-line sense organs and dermal surface structures of the tongue sole Cynoglossus semilaevis . Acta Zoologica Sinica , 2007a . 53 ( 6 ): 1 113 - 1 120 . .

S Mangos , Y Liu , I A Drummond . Dynamic expression of the osmosensory channel trpv4 in multiple developing organs in zebrafish . Gene Expression Patterns , 2007 . 7 ( 4 ): 480 - 484 . DOI: 10.1016/j.modgep.2006.10.011 http://doi.org/10.1016/j.modgep.2006.10.011 .

N J Marshall . Vision and sensory physiology the lateral line systems of three deep-sea fish . Journal of Fish Biology , 1996 . 49 ( SA ): 239 - 258 . DOI: 10.1111/j.1095-8649.1996.tb06079.x http://doi.org/10.1111/j.1095-8649.1996.tb06079.x .

R G O'Neil , S Heller . The mechanosensitive nature of TRPV channels . Pflügers Archiv. , 2005 . 451 ( 1 ): 193 - 203 . DOI: 10.1007/s00424-005-1424-4 http://doi.org/10.1007/s00424-005-1424-4 .

S F Pedersen , G Owsianik , B Nilius . TRP channels: an overview . Cell Calcium , 2005 . 38 ( 3-4 ): 233 - 252 . DOI: 10.1016/j.ceca.2005.06.028 http://doi.org/10.1016/j.ceca.2005.06.028 .

X Rui , X Z S Xu . Mechanosensitive channels: in touch with Piezo . Current Biology , 2010 . 20 ( 21 ): R936 - R938 . DOI: 10.1016/j.cub.2010.09.053 http://doi.org/10.1016/j.cub.2010.09.053 .

D A Ryskamp , P Witkovsky , P Barabas , W Huang , C Koehler , N P Akimov , S H Lee , S Chauhan , W Xing , R C Rentería , W Liedtke , D Krizaj . The polymodal ion channel trpv4 modulates calcium flux, spiking rate and apoptosis of mouse retinal ganglion cells . Journal of Neuroscience , 2014 . 31 ( 19 ): 7 089 - 7 101 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2c4e41b5ca4dd77050d1d4a16fbb5f83 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2c4e41b5ca4dd77050d1d4a16fbb5f83 , .

Z X Sha , X H Luo , X L Liao , SL Wang , Q L Wang , S L Chen . Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis . Journal of Fish Biology , 2011 . 78 ( 1 ): 322 - 331 . DOI: 10.1111/j.1095-8649.2010.02793.x http://doi.org/10.1111/j.1095-8649.2010.02793.x .

R Strotmann , C Harteneck , K Nunnenmacher , G Schultz , T D Plant . OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity . Nature Cell Biology , 2000 . 2 ( 10 ): 695 - 702 . DOI: 10.1038/35036318 http://doi.org/10.1038/35036318 .

Suzuki M, Mizuno A. 2012. The molecular mechanism of multifunctional mechano-gated channel TRPV4. In : Amkin A, Lozinsky I eds. Mechanically Gated Channels and their Regulation. Springer, Dordrecht.

C Thisse , B Thisse . High-resolution in situ hybridization to whole-mount zebrafish embryos . Nature Protocols , 2008 . 3 ( 1 ): 59 - 69 . DOI: 10.1038/nprot.2007.514 http://doi.org/10.1038/nprot.2007.514 .

T Voets , J Prenen , J Vriens , H Watanabe , A Janssens , U Wissenbach , M Bödding , G Droogmans , B Nilius . Molecular determinants of permeation through the cation channel TRPV4 . Journal of Biological Chemistry , 2002 . 277 ( 37 ): 33 704 - 33 710 . DOI: 10.1074/jbc.M204828200 http://doi.org/10.1074/jbc.M204828200 .

J Vriens , H Watanabe , A Janssens , G Droogmans , T Voets , B Nilius . Cell swelling, heat, and chemical agonists use distinct pathways for the activation of the cation channel trpv4 . Proceedings of the National Academy of Sciences of the United States of America , 2004 . 101 ( 1 ): 396 - 401 . DOI: 10.1073/pnas.0303329101 http://doi.org/10.1073/pnas.0303329101 .

S Watanabe , A P Seale , E G Grau , T Kaneko . Stretchactivated cation channel TRPV4 mediates hyposmotically induced prolactin release from prolactin cells of Mozambique tilapia Oreochromismossambicus . American Journal of Physiology Regulatory Integrative & Comparative Physiology , 2012 . 302 ( 8 ): R1 004 - R1 011 . .

U Wissenbach , M Bödding , M Freichel , V Flockerzi . Trp12, a novel Trp related protein from kidney . FEBS Letters , 2000 . 485 ( 2-3 ): 127 - 134 . DOI: 10.1016/S0014-5793(00)02212-2 http://doi.org/10.1016/S0014-5793(00)02212-2 .

Y A Zhang , A Okada , C H Lew , S K Mcconnell . Regulated nuclear trafficking of the homeodomain protein otx1 in cortical neurons . Molecular and Cellular Neuroscience , 2002 . 19 ( 3 ): 430 - 446 . DOI: 10.1006/mcne.2001.1076 http://doi.org/10.1006/mcne.2001.1076 .

Z Zhang , Z Zhao , R F Margolskee , E Liman . The transduction channel TRPM5 is gated by intracellular calcium in taste cells . Neuroscience , 2007 . 27 ( 21 ): 5 777 - 5 786 . DOI: 10.1523/JNEUROSCI.4973-06.2007 http://doi.org/10.1523/JNEUROSCI.4973-06.2007 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency

Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney

Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

Genome-wide identification and prediction of long noncoding RNAs in half-smooth tongue sole Cynoglossus semilaevis

iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

Related Author

Junqiong SHI

Shuhan HE

Lu ZHAO

Lulu JI

Songqi YANG

Zhongxing WU

Yanan LOU

Bin SUN

Related Institution

Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology

University of Chinese Academy of Sciences

Tianjin Weishan Road Middle School

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

DOI：10.1007/s00343-019-8316-5

摘要

Abstract

关键词

Keywords

references