High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber <italic style="font-style: italic">Apostichopus japonicus</italic> feeding with three antibiotics

Ye ZHAO; Qing WANG; Hui LIU; Bingjun LI; Hongxia ZHANG

doi:10.1007/s00343-019-8308-5

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High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics

Aquaculture and Fisheries | Updated：2023-05-20

- High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics
- Journal of Oceanology and Limnology Vol. 37, Issue 5, Pages: 1714-1725(2019)
- Affiliations：
  
  1.Ocean School, Yantai University, Yantai 264005, China
  2.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
- Author bio：
  
  ZHAO Ye, zyzy19872006@ytu.edu.cn
  LIU Hui, huiliu@yic.ac.cn
- Funds：
- DOI：10.1007/s00343-019-8308-5
  CLC：
- Received：22 October 2018，
  
  Accepted：07 December 2018，
  
  Online First：20 December 2018，
  
  Published：2019-09
- Accepted：
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Ye ZHAO, Qing WANG, Hui LIU, et al. High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1714-1725.
DOI：

Ye ZHAO, Qing WANG, Hui LIU, et al. High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1714-1725. DOI： 10.1007/s00343-019-8308-5.

摘要

Abstract

Sea cucumber

Apostichopus japonicus

is an important marine economic species in Asian countries due to its profound nutritional and medicinal value. So far

with the rapid development of intensified artificial aquaculture of sea cucumbers

the use of antibiotics is still an inexpensive and dispensable way to treat pathogenic infections

especially during the nursery phase. However

there is little information on the effects of antibiotics on the intestinal microbiota of sea cucumber. Therefore an Illumina based sequencing method was used to examine the intestinal bacterial composition of juvenile

A. japonicas

following diets with three typical antibiotics (tetracycline

erythromycin

and norfloxacin) under 15

and 45 d. The findings reveal that different antibiotics have distinct effects on the growth performance of juvenile sea cucumbers. However

the richness and diversity of microbiota were barely affected by antibiotics but the community composition alterations indicated that the three antibiotics exhibited their respective patterns of reshaping the intestinal bacteria of juvenile sea cucumbers. In common

the abundance of some sensitive genera with helpful functions

such as

Thalassotalea

Shewanella

Sulfitobacter

and

Halomonas

decreased significantly with exposure to antibiotics and the abundance of multiple potential pathogenic- and suspected antibiotic-resistant microorganisms like

Arcobacter

Leucothrix

and

Clostridium_sensu_stricto_1

was found increased significantly in the antibiotic groups. These results suggest that low doses of antibiotics could affect the composition of the intestinal microbiota of sea cucumbers and might increase the risk of infection of the hosts. This study could help us to explore how antibacterial compounds modify the gut microbiota of sea cucumbers and provide theoretical guidance in hatchery management by scientific antibiotic use in sea cucumber mariculture.

关键词

Keywords

references

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Related Institution

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High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics

DOI：10.1007/s00343-019-8308-5

摘要

Abstract

关键词

Keywords

references