Effect of carbon and nitrogen ratio control on <italic style="font-style: italic">Artemia</italic> growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition

Shanyue WANG; Xueping CUI; Ruyi XU; Meirong GAO; Liying SUI

doi:10.1007/s00343-019-8288-5

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Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition

Aquaculture and Fisheries | Updated：2023-05-20

- Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition
- Journal of Oceanology and Limnology Vol. 37, Issue 5, Pages: 1768-1776(2019)
- Affiliations：
  
  Asian Regional Artemia Reference Center, Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
- Author bio：
  
  SUI Liying, suily@tust.edu.cn
- Funds：
- DOI：10.1007/s00343-019-8288-5
  CLC：
- Received：05 October 2018，
  
  Accepted：15 November 2018，
  
  Online First：13 February 2019，
  
  Published：2019-09
- Accepted：
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Shanyue WANG, Xueping CUI, Ruyi XU, et al. Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1768-1776.
DOI：

Shanyue WANG, Xueping CUI, Ruyi XU, et al. Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1768-1776. DOI： 10.1007/s00343-019-8288-5.

摘要

Abstract

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon

which reduces the nitrogen level in the water and provides microbial protein to the animals. However

most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp

Artemia

that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on

Artemia

growth

water quality

and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5

and 30 (Su5

Su15

and Su30)

respectively. The culture without adding sucrose was used as a control.

Artemia

was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) significantly improved the

Artemia

survival

growth and water quality (

0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV) (

0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness

and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.

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Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition

DOI：10.1007/s00343-019-8288-5

摘要

Abstract

关键词

Keywords

references