Induction of gyno-tetraploidy in Japanese flounder <italic style="font-style: italic">Paralichthys olivaceus</italic>

Guixing WANG; Xiaoyan ZHANG; Zhaohui SUN; Yaxian ZHAO; Wei DU; Jianbin CUI; Jilun HOU; Yufen WANG

doi:10.1007/s00343-019-8252-4

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Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus

Aquaculture and Fisheries | Updated：2023-04-27

- Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus
- Journal of Oceanology and Limnology Vol. 38, Issue 1, Pages: 288-293(2020)
- Affiliations：
  
  1.Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Beijing 100141, China
  2.Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China
  3.Ocean College, Agricultural University of Hebei, Qinhuangdao 066009, China
- Author bio：
  
  HOU Jilun, jilunhou@126.com
  WANG Yufen, wangyf-8000@163.com
- Funds：
- DOI：10.1007/s00343-019-8252-4
  CLC：
- Received：24 September 2018，
  
  Accepted：10 January 2019，
  
  Online First：21 March 2019，
  
  Published：2020-01
- Accepted：
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Guixing WANG, Xiaoyan ZHANG, Zhaohui SUN, et al. Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2020, 38(1): 288-293.
DOI：

Guixing WANG, Xiaoyan ZHANG, Zhaohui SUN, et al. Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2020, 38(1): 288-293. DOI： 10.1007/s00343-019-8252-4.

摘要

Abstract

Tetraploid fish are important for mass production of triploids in polyploid breeding. In this study

we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder

Paralichthys olivaceus

. The induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream

Pagrus major

administering a cold shock (0℃

5 to 45 min) 3 min after fertilization to inhibit second polar body exclusion

incubating the eggs for 60 min at 17℃

and treating them with a 650 kg/cm

hydrostatics pressure shock for 6 min. We named the embryos gyno-tetraploids that developed from eggs after such treatments. The hatching rate of the gyno-tetraploids ranged from 20.99%±3.66% to 36.01%±2.79%

and the tetraploid rate ranged from 80.00% to 100.00%. All-maternal inheritance was verified using 6 high-recombination-rate microsatellite markers. This method successfully induced gyno-tetraploidy. The successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.

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Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus

DOI：10.1007/s00343-019-8252-4

摘要

Abstract

关键词

Keywords

references