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Molecular analyses of bacterioplankton communities with highly abundant
Vibrio clades: a case study in Bohai Sea coastal waters- Journal of Oceanology and Limnology Vol. 37, Issue 5, Pages: 1638-1648(2019)
- Affiliations：
1.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
2.Institute for Advanced Study, Shenzhen University, Shenzhen 518000, China
3.College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
- Author bio：
ZHANG Yuan, zhangyuan1970craes@163.com
LI Meng, limeng848@szu.edu.cn
- Funds：
DOI：10.1007/s00343-019-8210-1
CLC：Received：19 August 2018，
Accepted：13 December 2018，
Online First：25 February 2019，
Published：2019-09
- Accepted：
Scan QR Code
Shuping WANG, Yuan ZHANG, Jia HE, et al. Molecular analyses of bacterioplankton communities with highly abundant
Vibrio clades: a case study in Bohai Sea coastal waters[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1638-1648.Shuping WANG, Yuan ZHANG, Jia HE, et al. Molecular analyses of bacterioplankton communities with highly abundant
Vibrio clades: a case study in Bohai Sea coastal waters[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1638-1648. DOI： 10.1007/s00343-019-8210-1.
摘要
Abstract
We investigated the bacterioplankton abundance
community composition and the associated Vibrio clades of natural seawater in Bohai Sea coastal waters. Seawater samples (10 L in triplicate) were collected at 0.5
3
and 5 m depths near the coastal aquaculture zone of the Bohai Sea on May 12
2016. Real-time PCR and 16S rRNA gene amplicon high-throughput sequencing methods were employed by which 485 operational taxonomic units (OTUs) at a 97% sequence similarity level were generated. Gammaproteobacteria
Alphaproteobacteria
and Bacteroidetes were the most abundant groups
accounting for 49.5%
23.5%
and 18.9% of the total assemblage
respectively. Obvious variations in
Pseudoalteromonas
Vibrio
and
Octadecabacter
which were the most abundant genera
could be observed among different samples. Notably
the results of
Vibrio
-specific real-time PCR indicated that
Vibrio
had extremely high 16S rRNA gene copy numbers. The 16S rRNA gene sequencing results across all the samples also indicated that they occupied a large proportion of the total assemblage. Both the alpha diversity and major bacterioplankton group
Pseudoalteromonas
had significant correlations with the concentration of PO
4
3-
. Overall
studies on bacterioplankton communities with highly abundant
Vibrio
clades can provide interesting insight into the microbial function and health assessment of the Bohai Sea coastal ecosystem.
关键词
Keywords
references
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