Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically

Yeguang Li; Fengping Miao; Yahong Geng; Dayan Lu; Chengwu Zhang; Mingtao Zeng

doi:10.1007/s00343-012-1217-5

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Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically

Updated：2023-05-16

- Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically
- Journal of Oceanology and Limnology Vol. 30, Issue 4, Pages: 627-637(2012)
- Affiliations：
  
  1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture Wuhan Botanical Garden Chinese Academy of Sciences
  2. Laboratory of Coastal Zone Bioresources Yantai Institute of Costal Zone Research Chinese Academy of Sciences
  3. Institute of Hydrobiology Jinan University
  4. Department of Biomedical Sciences Paul L. Foster School of Medicine Texas Tech University Health Sciences Center
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1217-5
  CLC：
- Online First：02 July 2012，
  
  Published：2012
- Accepted：
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Li, Y., Miao, F., Geng, Y. et al. Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically., Chin. J. Ocean. Limnol. 30, 627–637 (2012). https://doi.org/10.1007/s00343-012-1217-5
DOI：

Li, Y., Miao, F., Geng, Y. et al. Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically., Chin. J. Ocean. Limnol. 30, 627–637 (2012). https://doi.org/10.1007/s00343-012-1217-5 DOI：

摘要

Abstract

The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated

and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba’s method

alkali treatment destroys chlorophyll. However

we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520–550 nm. Interestingly

a good linear relationship existed between absorbance at 530 nm and astaxanthin content

while an unknown interference at 540–550 nm was detected in our study. Therefore

with 530 nm as working wavelength

the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll

other carotenoids

and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm

the measured values at 530 nm decreased by 6.93% and 11.96%

respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.

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references

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