Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

Jingjing Zhang; Quanbin Zhang; Jing Wang; Xuelian Shi; Zhongshan Zhang

doi:10.1007/s00343-009-9205-0

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Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

Updated：2023-05-16

- Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 578-582(2009)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9205-0
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Zhang, J., Zhang, Q., Wang, J. et al. Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC., Chin. J. Ocean. Limnol. 27, 578–582 (2009). https://doi.org/10.1007/s00343-009-9205-0
DOI：

Zhang, J., Zhang, Q., Wang, J. et al. Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC., Chin. J. Ocean. Limnol. 27, 578–582 (2009). https://doi.org/10.1007/s00343-009-9205-0 DOI：

摘要

Abstract

We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard

the monosaccharide derivatives

obtained with 1-Phenyl-3-methyl-5-pyrazolone (PMP)

were separated by reverse-phase HPLC using a gradient elution process

and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1–2.0 mmol/L

the peak area of each monosaccharide had a good linear relationship with its concentration (r 2>0.998). The average recoveries of mannose

rhamnose

glucuronic acid

glucose

galactose

xylose

and fucose were 86.2%

95.1%

62.5%

102.0%

94.8%

66.6%

and 105.1%

respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.

关键词

Keywords

references

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College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology

University of Chinese Academy of Sciences

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