Down-regulation of <italic style="font-style: italic">SEIPIN</italic> transcription attenuated the triacylglycerol accumulation in <italic style="font-style: italic">Nannochloropsis</italic> <italic style="font-style: italic">oceanica</italic>

Lixian LIU; Li GUO; Hang LIU; Xiaohui PAN; Yanan ZONG; Leili FENG; Guanpin YANG

Your Location：

Home >

Browse articles >

Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica

Biology | Updated：2025-02-19

- Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica
- Journal of Oceanology and Limnology Vol. 43, Issue 1, Pages: 187-195(2025)
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  3.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
  4.MoE Laboratory of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  qdguoli@139.com
  yguanpin@ouc.edu.cn
- Funds：
- DOI：
  CLC：
- Received：24 December 2023，
  
  Published：01 January 2025
- Accepted：
Scan QR Code
LIU Lixian,GUO Li,LIU Hang,et al.Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica[J].Journal of Oceanology and Limnology,2025,43(01):187-195.
DOI：

LIU Lixian,GUO Li,LIU Hang,et al.Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica[J].Journal of Oceanology and Limnology,2025,43(01):187-195. DOI：

摘要

Abstract

Abnormal content of protein SEIPIN causes Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) in humans. Previous studies have shown that

Arabidopsis

thaliana

has evolved three SEIPIN homologs

determining the fusion and size of lipid droplets.

Nannochloropsis

oceanica

is a model oleaginous microalga with the accumulation potential of fatty acids. We believe that the SEIPIN homolog if any in

oceanica

should function as it does in humans

plant among others. In this study

we identified a homologous gene of

SEIPIN

oceanica

constructed a bacterial plasmid containing the elements controlling the transcription of its complete antisense RNA

and integrated the linearized plasmid into the

oceanica

genome. We obtained a transformant in which the abundance of

SEIPIN

transcript is significantly down-regulated. Flow cytometry analysis showed that the triacylglycerol (TAG) content of the transformant is significantly lower than that of the wild type (WT). Transcriptomic analysis showed that the down-regulation of

SEIPIN

transcript abundance could affect the protein functioning in endoplasmic reticulum (ER)

implying that

SEIPIN

gene can function in

oceanica

in a similar way to that in

thaliana

关键词

Keywords

references

Bauen A , Berndes G , Junginger M et al . 2009 . Bioenergy-a sustainable and reliable energy source. A review of status and prospects . IEA Bioenergy ExCo , 05 .

Berardinelli W . 1954 . An undiagnosed endocrinometabolic syndrome: report of 2 cases . Journal of Clinical Endocrinology & Metabolism , 14 ( 2 ): 193 - 204 , https://doi.org/10.1210/jcem-14-2-193 https://doi.org/10.1210/jcem-14-2-193 .

Binns D , Lee S , Hilton C L et al . 2010 . Seipin is a discrete homooligomer . Biochemistry , 49 ( 50 ): 10747 - 10755 , https://doi.org/10.1021/bi1013003 https://doi.org/10.1021/bi1013003 .

Brennan L , Fernández A B , Mostaert A S et al . 2012 . Enhancement of BODIPY 505 / 515 lipid fluorescence method for applications in biofuel-directed microalgae production. Journal of Microbiological Methods , 90 ( 2 ): 137 - 143 , https://doi.org/10.1016/j.mimet.2012.03.020 https://doi.org/10.1016/j.mimet.2012.03.020 .

Cai Y Q Goodman J M , Pyc M et al . 2015 . Arabidopsis SEIPIN proteins modulate triacylglycerol accumulation and influence lipid droplet proliferation . The Plant Cell , 27 ( 9 ): 2616 - 2636 , https://doi.org/10.1105/tpc.15.00588 https://doi.org/10.1105/tpc.15.00588 .

Cooper M S , Hardin W R , Petersen T W et al . 2010 . Visualizing “green oil” in live algal cells . Journal of Bioscience and Bioengineering , 109 ( 2 ): 198 - 201 , https://doi.‍org/10.1016/j.jbiosc.2009.08.004 https://doi.‍org/10.1016/j.jbiosc.2009.08.004 . https://do 10.1016/j.jbiosc.2009.08.004 http://dx.doi.org/10.1016/j.jbiosc.2009.08.004

Fei W H , Shui G H , Gaeta B et al . 2008 . Fld 1 p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast. Journal of Cell Biology , 180 ( 3 ): 473 - 482 , https://doi.org/10.1083/jcb.200711136 https://doi.org/10.1083/jcb.200711136 .

Folch J , Lees M , Stanley G H S . 1957 . A simple method for the isolation and purification of total lipides from animal tissues . Journal of Biological Chemistry , 226 ( 1 ): 497 - 509 , https://doi.org/10.1016/S0021-9258(18)64849-5 https://doi.org/10.1016/S0021-9258(18)64849-5 .

Gibson D G . 2011 . Enzymatic assembly of overlapping DNA fragments . Methods in Enzymology , 498 : 349 - 361 , https://doi.org/10.1016/B978-0-12-385120-8.00015-2 https://doi.org/10.1016/B978-0-12-385120-8.00015-2 .

Guillard R R L . 1975 . Culture of phytoplankton for feeding marine invertebrates . In: Smith W L, Chanley M H eds. Culture of Marine Invertebrate Animals . Springer, Boston . p. 29 - 60 . https://do 10.1007/978-1-4615-8714-9_3 http://dx.doi.org/10.1007/978-1-4615-8714-9_3

Guo L , Liang S J , Zhang Z Y et al . 2019 . Genome assembly of Nannochloropsis oceanica provides evidence of host nucleus overthrow by the symbiont nucleus during speciation . Communications Biology , 2 ( 1 ): 249 , https://doi.org/10.1038/s42003-019-0500-9 https://doi.org/10.1038/s42003-019-0500-9 .

Hall T A . 1999 . BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95 / 98 /NT. Nucleic Acids Symposium Series , 41 : 95 - 98 , https://doi.org/10.1021/bk-1999-0734.ch008 https://doi.org/10.1021/bk-1999-0734.ch008 .

Hu H H , Gao K S . 2006 . Response of growth and fatty acid compositions of Nannochloropsis sp. to environmental factors under elevated CO 2 concentration . Biotechnology Letters , 28 ( 13 ): 987 - 992 , https://doi.‍‍org/10.1007/s10529-006-9026-6 https://doi.‍‍org/10.1007/s10529-006-9026-6 . https://do 10.1093/molehr/gah062 http://dx.doi.org/10.1093/molehr/gah062

James A O , Pedro C . 2019 . Dynamics and functions of lipid droplets . Nature Reviews Molecular Cell Biology , 20 ( 3 ): 137 - 155 , https://doi.org/10.1038/s41580-018-0085-z https://doi.org/10.1038/s41580-018-0085-z .

Kaye Y , Grundman O , Leu S et al . 2015 . Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica : overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG . Algal Research , 11 : 387 - 398 , https://doi.org/10.1016/j.algal.2015.05.003 https://doi.org/10.1016/j.algal.2015.05.003 .

Kim D , Langmead B , Salzberg S L . 2015 . HISAT: a fast spliced aligner with low memory requirements . Nature Methods , 12 ( 4 ): 357 - 360 , https://doi.org/10.1038/nmeth.3317 https://doi.org/10.1038/nmeth.3317 .

Lepage G , Roy C C . 1984 . Improved recovery of fatty acid through direct transesterification without prior extraction or purification . Journal of Lipid Research , 25 ( 12 ): 1391 - 1396 , https://doi.org/10.1016/S0022-2275(20)34457-6 https://doi.org/10.1016/S0022-2275(20)34457-6 .

Li D W , Cen S Y , Liu Y H et al . 2016 . A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica . Journal of Biotechnology , 229 : 65 - 71 , https://doi.org/10.1016/j.jbiotec.2016.05.005 https://doi.org/10.1016/j.jbiotec.2016.05.005 .

Livak K J , Schmittgen T D . 2001 . Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔ C t method . Methods , 25 ( 4 ): 402 - 408 , https://doi.org/10.1006/meth.2001.1262 https://doi.org/10.1006/meth.2001.1262 .

Love M I , Huber W , Anders S . 2014 . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 . Genome Biology , 15 ( 12 ): 550 , https://doi.org/10.1186/s13059-014-0550-8 https://doi.org/10.1186/s13059-014-0550-8 .

Lundin C , Nordström R , Wagner K et al . 2006 . Membrane topology of the human seipin protein . FEBS Letters , 580 ( 9 ): 2281 - 2284 , https://doi.‍org/10.‍1016/j.febslet.‍2006.03.040 https://doi.‍org/10.‍1016/j.febslet.‍2006.03.040 . https://do 10.1016/j.febslet.2006.03.040 http://dx.doi.org/10.1016/j.febslet.2006.03.040

Ma X N , Chen T P , Yang B et al . 2016 . Lipid production from Nannochloropsis . Marine Drugs , 14 ( 4 ): 61 , https://doi.org/10.3390/md14040061 https://doi.org/10.3390/md14040061 .

Ma Y B , Wang Z Y , Yu C J et al . 2014 . Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production . Bioresource Technology , 167 : 503 - 509 , https://doi.org/10.1016/j.biortech.2014.06.047 https://doi.org/10.1016/j.biortech.2014.06.047 .

Maity S K . 2015 . Opportunities, recent trends and challenges of integrated biorefinery: part I . Renewable and Sustainable Energy Reviews , 43 : 1427 - 1445 , https://doi.org/10.1016/j.rser.2014.11.092 https://doi.org/10.1016/j.rser.2014.11.092 .

Pertea M , Pertea G M , Antonescu C M et al . 2015 . StringTie enables improved reconstruction of a transcriptome from RNA-seq reads . Nature Biotechnology , 33 ( 3 ): 290 - 295 , https://doi.org/10.1038/nbt.3122 https://doi.org/10.1038/nbt.3122 .

Seip M , Trygstad O . 1996 . Generalized lipodystrophy, congenital and acquired (lipoatrophy) . Acta Paediatrica , 85 ( S413 ): 2 - 28 , https://doi.org/10.1111/j.1651-2227.1996.tb14262.x https://doi.org/10.1111/j.1651-2227.1996.tb14262.x .

Seip M . 1959 . Lipodystrophy and gigantism with associated endocrine manifestations. A new diencephalic syndrome? Acta Paediatrica , 48 : 555 - 574 . https://do 10.1111/j.1651-2227.1959.tb17558.x http://dx.doi.org/10.1111/j.1651-2227.1959.tb17558.x

Sui X , Arlt H , Brock K P et al . 2018 . Cryo-electron microscopy structure of the lipid droplet-formation protein seipin . The Journal of Cell Biology , 217 ( 12 ): 4080 - 4091 , https://doi.org/10.1083/jcb.201809067 https://doi.org/10.1083/jcb.201809067 .

Szymanski K M , Binns D , Bartz R et al . 2007 . The lipodystrophy protein seipin is found at endoplasmic reticulum lipid droplet junctions and is important for droplet morphology . Proceedings of the National Academy of Sciences of the United States of America , 104 ( 52 ): 20890 - 20895 , https://doi.org/10.1073/pnas.0704154104 https://doi.org/10.1073/pnas.0704154104 .

Taurino M , Costantini S , Domenico S D et al . 2018 . SEIPIN proteins mediate lipid droplet biogenesis to promote pollen transmission and reduce seed dormancy . Plant Physiology , 176 ( 2 ): 1531 - 1546 , https://doi.org/10.1104/pp.17.01430 https://doi.org/10.1104/pp.17.01430 .

Tian Y , Bi J F , Shui G Het al . 2011 . Tissue-autonomous function of Drosophila Seipin in preventing ectopic lipid droplet formation . PLoS Genetics , 7 ( 4 ): e1001364 , https://doi.org/10.1371/journal.pgen.1001364 https://doi.org/10.1371/journal.pgen.1001364 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Changes of pigments and lipids composition in Haematococcus pluvialis vegetative cell as affected by monochromatic red light compared with white light

Parametric Model of Lubricant Particle Trajectory inAnnulus Inter-Spools

Recent Advances of Atomization Characteristics under Oscillating Backpressure Conditions in Liquid Rocket Engines

Multivariable Constrained Model Predictive Control ofAero-Engine Based on Augmented Predictive Model

Related Author

Zhongyi ZHANG

Jie LUO

Xiaojuan ZHU

Chengxu ZHOU

Jianyuan WANG

Ran MENG

Jilin XU

Juanjuan CHEN

Related Institution

Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University

Department of Marine Drug and Biological Products, Ningbo Institute of Oceanography

Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University

School of Marine Science, Ningbo University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica

DOI：

摘要

Abstract

关键词

Keywords

references