17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in <italic style="font-style: italic">Takifugu fasciatus</italic>

Xuanyin CHEN; Sheng LUO; Yanfang LI; Rui LI; Xiaojun LI; Zhengyang HU; Shaowu YIN; Tao WANG

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17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in Takifugu fasciatus

Biology | Updated：2025-02-19

- 17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in Takifugu fasciatus
- Journal of Oceanology and Limnology Vol. 43, Issue 1, Pages: 219-231(2025)
- Affiliations：
  
  1.College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing 210023, China
  2.Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang 222005, China
- Author bio：
  
  seawater88@126.com
- Funds：
- DOI：
  CLC：
- Received：16 January 2024，
  
  Published：01 January 2025
- Accepted：
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CHEN Xuanyin,LUO Sheng,LI Yanfang,et al.17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in Takifugu fasciatus[J].Journal of Oceanology and Limnology,2025,43(01):219-231.
DOI：

CHEN Xuanyin,LUO Sheng,LI Yanfang,et al.17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in Takifugu fasciatus[J].Journal of Oceanology and Limnology,2025,43(01):219-231. DOI：

摘要

Abstract

17α-methyltestosterone (17α-MT) is an emerging pollutant

which is harmful to the endocrine system and reproduction of fish. We investigated the effects of different concentrations of 17α-MT (0

and 100 mg/kg) on endoplasmic reticulum stress (ERS) and apoptosis in the liver of

Takifugu

fasciatus

. Results show that: (1) with the increase of 17α-MT treatment concentration

liver transaminases (alanine aminotransferase; aspartate aminotransferase) and the mRNA expression of ERS marker genes (glucose-regulated protein 78; calreticulin) of

fasciatus

were significantly increased compared with the control group (

0.05); (2) the activity of succinate dehydrogenase (SDH)

Caspase3 and Caspase9 in the liver of

fasciatus

increased with the increase of 17α-MT concentration compared with the control group (

0.05); (3) by using 4-phenylbutyricacid (4-PBA) inhibitors to stimulate ERS through in vitro experiments

the expression of ERS

and apoptosis-related genes significantly decreased (

0.05)

and the apoptosis rate of

fasciatus

hepatocytes was significantly inhibited (

0.05) under 17α-MT treatment. This study confirmed that ERS played an important role in the induction of apoptosis in the hepatocytes of

fasciatus

which enriched the ecotoxicological information of environmental androgens.

关键词

Keywords

references

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17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in Takifugu fasciatus

DOI：

摘要

Abstract

关键词

Keywords

references