Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster <italic style="font-style: italic">Crassostrea gigas</italic>

Qiang FU; Zichao YU; Junyan ZHAO; Lei GAO; Ning KONG; Lingling WANG; Linsheng SONG

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Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster Crassostrea gigas

Aquaculture and Fisheries | Updated：2024-10-11

- Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster Crassostrea gigas
- Journal of Oceanology and Limnology Vol. 42, Issue 4, Pages: 1348-1358(2024)
- Affiliations：
  
  1.Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China
  2.Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China
  4.Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China
- Author bio：
  
  kongning@dlou.edu.cn;
  wanglingling@dlou.edu.cn
- Funds：
- DOI：
  CLC：
- Received：18 September 2023，
  
  Online First：15 December 2023，
  
  Published：01 July 2024
- Accepted：
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FU Qiang,YU Zichao,ZHAO Junyan,et al.Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster ,Crassostrea gigas[J].Journal of Oceanology and Limnology,2024,42(04):1348-1358.
DOI：

FU Qiang,YU Zichao,ZHAO Junyan,et al.Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster ,Crassostrea gigas[J].Journal of Oceanology and Limnology,2024,42(04):1348-1358. DOI：

摘要

Abstract

The Pacific oyster

Crassostrea

gigas

one of the most exploited molluscs in the world

has suffered from massive mortality in recent decades

and the occurrence mechanisms have not been well-characterized. In this study

to reveal the relationship of associated microbiota to the fitness of oysters

temporal dynamics of microbiota in the gill

hemolymph

and hepatopancreas of

gigas

during April 2018‍–‍January 2‍019 were investigated by

16S rRNA gene sequencing. The microbiota in

gigas

exhibited tissue heterogeneity

of which Spirochaetaceae was dominant in the gill and hemolymph while Mycoplasmataceae enriched in the hepatopancreas. Co-occurrence network demonstrated that the gill microbiota exhibited higher inter-taxon connectivity while the hemolymph microbiota had more modules. The richness (Chao1 index) and diversity (Shannon index) of microbial community in each tissue showed no significant seasonal variations

except for the hepatopancreas having a higher richness in the autumn. Similarly

beta diversity analysis indicated a relatively stable microbiota in each tissue during the sampling period

showing relative abundance of the dominant taxa exhibiting temporal dynamics. Results indicate that the microbial community in

gigas

showed a tissue-specific stability with temporal dynamics in the composition

which might be essential for the tissue functioning and environmental adaption in oysters. This work provides a baseline microbiota in

gigas

and is helpful for the understanding of host-microbiota interaction in oysters.

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Keywords

references

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Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster Crassostrea gigas

DOI：

摘要

Abstract

关键词

Keywords

references