Proliferating cell nuclear antigen of <italic style="font-style: italic">Ulva</italic><italic style="font-style: italic">prolifera</italic> is involved in the response to temperature stress

Hongyan HE; Juanjuan YANG; Yuan HE; Xiuwen YANG; Caiwei FU; Dongren ZHANG; Jingwei DONG; Aurang ZEB; Jing QU; Songdong SHEN

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Proliferating cell nuclear antigen of Ulvaprolifera is involved in the response to temperature stress

Ecology | Updated：2024-10-11

- Proliferating cell nuclear antigen of Ulvaprolifera is involved in the response to temperature stress
- Journal of Oceanology and Limnology Vol. 42, Issue 4, Pages: 1227-1241(2024)
- Affiliations：
  
  1. School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215101, China
  2. Department of Pathology, Affiliated Hospital and Medical School of Nantong University, Nantong 226001, China
- Author bio：
  
  shensongdong@suda.edu.cn
- Funds：
- DOI：
  CLC：
- Received：24 August 2023，
  
  Online First：04 November 2023，
  
  Published：01 July 2024
- Accepted：
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HE Hongyan,YANG Juanjuan,HE Yuan,et al.Proliferating cell nuclear antigen of Ulva, prolifera is involved in the response to temperature stress[J].Journal of Oceanology and Limnology,2024,42(04):1227-1241.
DOI：

HE Hongyan,YANG Juanjuan,HE Yuan,et al.Proliferating cell nuclear antigen of Ulva, prolifera is involved in the response to temperature stress[J].Journal of Oceanology and Limnology,2024,42(04):1227-1241. DOI：

摘要

Abstract

Ulva

prolifera

is the most common specie causative to green tide

and its growth is sensitive to temperature stress. However

the mechanisms of

prolifera

response to temperature stress remain elusive. In this study

high temperature (36 °‍‍C) stimulus promoted the death of unformed cell wall protoplasts and delayed the division of formed cell wall protoplasts

while low-temperature (4 °‍‍C) stimulus did not

suggesting that the mechanisms of the response of

prolifera

to high and low-temperature stresses are different. Transcriptome results show that proliferation-related genes were differentially expressed under high and low-temperature stresses

especially the proliferating cell nuclear antigen (PCNA) and cyclins (CYCs). Subsequently

the interaction between PCNA and Cyclin A was confirmed by Co-immunoprecipitation

yeast two-hybrid

and so on. Furthermore

high- and low-temperature stresses induced the expression of

PCNA

and

Cyclin

in varying of degrees

and activated extracellular signal-regulated kinase (ERK) signal pathway. These results suggest

PCNA

Cyclin

and ERK signal pathway played important roles in the resistance of

prolifera

to temperature stress. Interestingly

high-temperature stress induced an increase of miR-2916 in abundance

and exhibiting reverse expression of

PCNA

; and PCNA was target gene of miR-2916

suggesting that miR-2916 protected

prolifera

from high-temperature stress via post-transcriptionally regulation of PCNA. This study laid a foundation for understanding the function of

PCNA

and

Cyclin

moreover

it has a guiding significance to explore the mechanisms of the response to temperature stress from proliferation-related genes regulatory networks in

prolifera

关键词

Keywords

references

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Related Author

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Hongyan HE

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Songdong SHEN

Related Institution

Department of Cell Biology, School of Biology and Basic Medical Sciences, Soochow University

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Proliferating cell nuclear antigen of Ulvaprolifera is involved in the response to temperature stress

DOI：

摘要

Abstract

关键词

Keywords

references