RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster <italic style="font-style: italic">Crassostrea</italic> <italic style="font-style: italic">hongkongensis</italic>

Yeshao PENG; Ziao CHEN; Qiong DENG; Zhen JIA; Lingxin KONG; Peng ZHU; Youhou XU; Zhicai SHE

Your Location：

Home >

Browse articles >

RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

Biology | Updated：2024-10-11

- RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis
- Journal of Oceanology and Limnology Vol. 42, Issue 4, Pages: 1261-1271(2024)
- Affiliations：
  
  Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Pinglu Canal and Beibu Gulf Coastal Ecosystem Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China
- Author bio：
  
  xuxuyoutiao@163.com
  shezc@bbgu.edu.cn
- Funds：
- DOI：
  CLC：
- Received：15 July 2023，
  
  Published：01 July 2024
- Accepted：
Scan QR Code
PENG Yeshao,CHEN Ziao,DENG Qiong,et al.RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostreahongkongensis[J].Journal of Oceanology and Limnology,2024,42(04):1261-1271.
DOI：

PENG Yeshao,CHEN Ziao,DENG Qiong,et al.RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostreahongkongensis[J].Journal of Oceanology and Limnology,2024,42(04):1261-1271. DOI：

摘要

Abstract

The chloride channel 7 gene (

CLC7

) of the Hong Kong oyster

Crassostrea

hongkongensis

was cloned and named

ChCLC7

. The cDNA was 2 572 bp in length

with a 5′ non-coding region containing 25 bp

a 3′ non-coding region containing 327 bp

and an open reading frame of 2 298 bp. ChCLC7 has 96.8% and 92.1% homology with CLC7 of

Crassostrea gigas

and

Crassostrea

virginica

respectively

and it was clustered with CLC7 of

gigas

and

virginica

. QRT-PCR showed that

ChCLC7

was expressed in all eight tissues

with the highest in adductor muscle and second in gill. The

ChCLC7

expression pattern in gill was altered significantly under high salinity stress with an overall upward and then downward trend. After RNA interference

the expression of

ChCLC7

and survival rate of oyster under high salinity stress was reduced significantly

and so did the concentration of hemolymph chloride ion in 48–96 h after RNA interference. We believed that

ChCLC7

could play an important role in osmoregulation of

hongkongensis

by regulating Cl‍⁻ transport. This study provided data for the analysis of molecular mechanism against oyster salinity stress.

关键词

Keywords

references

Bykova E A , Zhang X D , Chen T Y et al . 2006 . Large movement in the C terminus of CLC-0 chloride channel during slow gating . Nature Structural & Molecular Biology , 13 ( 12 ): 1115 - 1119 , https://doi.org/10.1038/nsmb1176 https://doi.org/10.1038/nsmb1176 .

Cerdà J , Chauvigné F , Finn R N . 2017 . The physiological role and regulation of aquaporins in teleost germ cells . In: Yang B X ed. Aquaporins . Springer, Dordrecht . p. 149 - 171 , https://doi.org/10.1007/978-94-024-1057-0_10 https://doi.org/10.1007/978-94-024-1057-0_10 .

Choi S H , Jee B Y , Lee S J et al . 2013 . Effects of RNA interference-mediated knock-down of hypoxia-inducible factor-α on respiratory burst activity of the Pacific oyster Crassostrea gigas hemocytes . Fish & Shellfish Immunology , 35 ( 2 ): 476 - 479 , https://doi.‍org/10.1016/j.fsi.2013.05.001 https://doi.‍org/10.1016/j.fsi.2013.05.001 . https://do 10.1016/j.fsi.2013.05.001 http://dx.doi.org/10.1016/j.fsi.2013.05.001

Chow S C , Wong C K C . 2011 . Regulatory function of hyperosmotic stress-induced signaling cascades in the expression of transcription factors and osmolyte transporters in freshwater Japanese eel primary gill cell culture . Journal of Experimental Biology , 214 ( 8 ): 1264 - 1270 , https://doi.org/10.1242/jeb.050435 https://doi.org/10.1242/jeb.050435 .

Estévez R , Pusch M , Ferrer-Costa C et al . 2004 . Functional and structural conservation of CBS domains from CLC chloride channels . The Journal of Physiology , 557 ( 2 ): 363 - 378 , https://doi.org/10.1113/jphysiol.2003.058453 https://doi.org/10.1113/jphysiol.2003.058453 .

Evans D H . 2009 . Osmotic and Ionic Regulation. CRC Press, Boca Raton . p . 115 - 123 , https://doi.org/10.1201/9780849380525 https://doi.org/10.1201/9780849380525 .

Fang A N P , Peng T C , Yen P K et al . 2016 . Effect of salinity on embryo and larval development of oyster Crassostrea iredalei . Tropical Life Sciences Research , 27 ( S1 ): 23 - 29 , https://doi.org/10.21315/tlsr2016.27.3.4 https://doi.org/10.21315/tlsr2016.27.3.4 .

Fong P , Rehfeldt A , Jentsch T J . 1998 . Determinants of slow gating in ClC-0, the voltage-gated chloride channel of Torpedo marmorata . American Journal of Physiology-Cell Physiology , 274 ( 4 ): C966 - C973 , https://doi.‍org/10.1152/ajpcell.1998.274.4.C966 https://doi.‍org/10.1152/ajpcell.1998.274.4.C966 . https://do 10.1152/ajpcell.1998.274.4.c966 http://dx.doi.org/10.1152/ajpcell.1998.274.4.c966

Freire C A , Amado E M , Souza L R et al . 2008 . Muscle water control in crustaceans and fishes as a function of habitat, osmoregulatory capacity, and degree of euryhalinity . Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology , 149 ( 4 ): 435 - 446 , https://doi.org/10.1016/j.cbpa.2008.02.003 https://doi.org/10.1016/j.cbpa.2008.02.003 .

Gagnon É , Forbush B , Caron L et al . 2003 . Functional comparison of renal Na-K-Cl cotransporters between distant species . American Journal of Physiology-Cell Physiology , 284 ( 2 ): C365 - C370 , https://doi.‍org/10.1152/ajpcell.00262.2002 https://doi.‍org/10.1152/ajpcell.00262.2002 . https://do 10.1152/ajpcell.00262.2002 http://dx.doi.org/10.1152/ajpcell.00262.2002

Hosoi M , Kubota S , Toyohara M et al . 2003 . Effect of salinity change on free amino acid content in Pacific oyster . Fisheries Science , 69 ( 2 ): 395 - 400 , https://doi.‍org/10.1046/j.1444-2906.2003.00634.x https://doi.‍org/10.1046/j.1444-2906.2003.00634.x . https://do 10.1046/j.1444-2906.2003.00634.x http://dx.doi.org/10.1046/j.1444-2906.2003.00634.x

Hosoi M , Shinzato C , Takagi M et al . 2007 . Taurine transporter from the giant Pacific oyster Crassostrea gigas : function and expression in response to hyper- and hypo-osmotic stress . Fisheries Science , 73 ( 2 ): 385 - 394 , https://doi.org/10.1111/j.1444-2906.2007.01346.x https://doi.org/10.1111/j.1444-2906.2007.01346.x .

Huang B Y , Zhang L L , Xu F et al . 2019 . Oyster versatile IKKα/βs are involved in toll-like receptor and RIG-I-like receptor signaling for innate immune response . Frontiers in Immunology , 10 : 1826 , https://doi.‍org/10.3389/fimmu.2019.01826 https://doi.‍org/10.3389/fimmu.2019.01826 . https://do 10.3389/fimmu.2019.01826 http://dx.doi.org/10.3389/fimmu.2019.01826

Jentsch T J . 2007 . Chloride and the endosomal-lysosomal pathway: emerging roles of CLC chloride transporters . The Journal of Physiology , 578 ( 3 ): 633 - 640 , https://doi.org/10.1113/jphysiol.2006.124719 https://doi.org/10.1113/jphysiol.2006.124719 .

Jentsch T J , Friedrich T , Schriever A et al . 1999 . The CLC chloride channel family . Pflügers Archiv European Journal of Physiology , 437 ( 6 ): 783 - 795 , https://doi.‍org/10.1007/s004240050847 https://doi.‍org/10.1007/s004240050847 . https://do 10.1007/s004240050847 http://dx.doi.org/10.1007/s004240050847

Jentsch T J , Stein V , Weinreich F et al . 2002 . Molecular structure and physiological function of chloride channels . Physiological Reviews , 82 ( 2 ): 503 - 568 , https://doi.org/10.1152/physrev.00029.2001 https://doi.org/10.1152/physrev.00029.2001 .

Kornak U , Kasper D , Bösl M R et al . 2001 . Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man . Cell , 104 ( 2 ): 205 - 215 , https://doi.‍org/10.1016/s0092-8674(01)00206-9 https://doi.‍org/10.1016/s0092-8674(01)00206-9 . https://do 10.1016/s0092-8674(01)00206-9 http://dx.doi.org/10.1016/s0092-8674(01)00206-9

Kurita T , Yamamura H , Suzuki Y et al . 2015 . The ClC-7 chloride channel is downregulated by hypoosmotic stress in human chondrocytes . Molecular Pharmacology , 88 ( 1 ): 113 - 120 , https://doi.org/10.1124/mol.115.098160 https://doi.org/10.1124/mol.115.098160 .

Lam K , Morton B . 2004 . The oysters of Hong Kong (Bivalvia: Ostreidae and Gryphaeidae) . The Raffles Bulletin of Zoology , 52 ( 1 ): 11 - 28 .

Leanza L , Biasutto L , Managò A et al . 2013 . Intracellular ion channels and cancer . Frontiers in Physiology , 4 : 227 , https://doi.org/10.3389/fphys.2013.00227 https://doi.org/10.3389/fphys.2013.00227 .

Lemoinne S , Thabut D , Housset C et al . 2014 . The emerging roles of microvesicles in liver diseases . Nature Reviews Gastroenterology & Hepatology , 11 ( 6 ): 350 - 361 , https://doi.org/10.1038/nrgastro.2014.7 https://doi.org/10.1038/nrgastro.2014.7 .

Li A , Li L , Song K et al . 2017 . Temperature, energy metabolism, and adaptive divergence in two oyster subspecies . Ecology and Evolution , 7 ( 16 ): 6151 - 6162 , https://doi.org/10.1002/ece3.3085 https://doi.org/10.1002/ece3.3085 .

Li J T , Ma P , Liu P et al . 2015 . The roles of Na + /K + -ATPase α-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses . Fish & Shellfish Immunology , 42 ( 2 ): 264 - 271 , https://doi.org/10.1016/j.fsi.2014.10.043 https://doi.org/10.1016/j.fsi.2014.10.043 .

Livak K J , Schmittgen T D . 2001 . Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔ C t method . Methods , 25 ( 4 ): 402 - 408 , https://doi.org/10.1006/meth.2001.1262 https://doi.org/10.1006/meth.2001.1262 .

McMains E , Krishnan V , Prasad S et al . 2011 . Expression and localization of CLC chloride transport proteins in the avian retina . PLoS One , 6 ( 3 ): e17647 , https://doi.org/10.1371/journal.pone.0017647 https://doi.org/10.1371/journal.pone.0017647 .

Piccoli M T , Gupta S K , Thum T . 2015 . Noncoding RNAs as regulators of cardiomyocyte proliferation and death . Journal of Molecular and Cellular Cardiology , 89 : 59 - 67 , https://doi.org/10.1016/j.yjmcc.2015.02.002 https://doi.org/10.1016/j.yjmcc.2015.02.002 .

Pierce S K . 1982 . Invertebrate cell volume control mechanisms: a coordinated use of intracellular amino acids and inorganic ions as osmotic solute . The Biological Bulletin , 163 ( 3 ): 405 - 419 , https://doi.‍org/10.2307/1541452 https://doi.‍org/10.2307/1541452 . https://do 10.2307/1541452 http://dx.doi.org/10.2307/1541452

Pierce S K , Rowland-Faux L M , O’Brien S M . 1992 . Different salinity tolerance mechanisms in Atlantic and Chesapeake Bay conspecific oysters: glycine betaine and amino acid pool variations . Marine Biology , 113 ( 1 ): 107 - 115 , https://doi.org/10.1007/bf00367644 https://doi.org/10.1007/bf00367644 .

Stauber T , Weinert S , Jentsch T J . 2012 . Cell biology and physiology of CLC chloride channels and transporters . Comprehensive Physiology , 2 ( 3 ): 1701 - 1744 , https://doi.org/10.1002/cphy.c110038 https://doi.org/10.1002/cphy.c110038 .

Subba A , Tomar S , Pareek A et al . 2021 . The chloride channels: silently serving the plants . Physiologia Plantarum , 171 ( 4 ): 688 - 702 , https://doi.org/10.1111/ppl.13240 https://doi.org/10.1111/ppl.13240 .

Tang X Y , Huang B Y , Lin S H et al . 2020 . CgMyD88s serves as an innate immune system plug during ostreid herpesvirus 1 infection in the Pacific Oyster ( Crassostrea gigas ) . Frontiers in Immunology , 11 : 1247 , https://doi.‍org/10.3389/fimmu.2020.01247 https://doi.‍org/10.3389/fimmu.2020.01247 . https://do 10.3389/fimmu.2020.01247 http://dx.doi.org/10.3389/fimmu.2020.01247

Tian Y , Jiang Y N , Shang Y P et al . 2017 . Establishment of lysozyme gene RNA interference systemand its involvement in salinity tolerance in sea cucumber ( Apostichopus japonicus ) . Fish & Shellfish Immunology , 65 : 71 - 79 , https://doi.org/10.1016/j.fsi.2017.03.046 https://doi.org/10.1016/j.fsi.2017.03.046 .

Toyohara H , Yoshida M , Hosoi M et al . 2005 . Expression of taurine transporter in response to hypo-osmotic stress in the mantle of Mediterranean blue mussel . Fisheries Science , 71 ( 2 ): 356 - 360 , https://doi.‍org/10.1111/j.‍1444-2906.2005.00972.x https://doi.‍org/10.1111/j.‍1444-2906.2005.00972.x . https://do 10.1111/j.1444-2906.2005.00972.x http://dx.doi.org/10.1111/j.1444-2906.2005.00972.x

Tse W K F , Lai K P , Takei Y . 2011 . Medaka osmotic stress transcription factor 1b (Ostf1b/TSC 22D3 - 2 ) triggers hyperosmotic responses of different ion transporters in medaka gill and human embryonic kidney cells via the JNK signalling pathway. The International Journal of Biochemistry & Cell Biology , 43 ( 12 ): 1764 - 1775 , https://doi.org/10.1016/j.biocel.2011.08.013 https://doi.org/10.1016/j.biocel.2011.08.013 .

Waldegger S , Jentsch T J . 2000 . From tonus to tonicity: physiology of CLC chloride channels . Journal of the American Society of Nephrology , 11 ( 7 ): 1331 - 1339 , https://doi.org/10.1681/ASN.V1171331 https://doi.org/10.1681/ASN.V1171331 .

Wang L L , Song X R , Song L S . 2018 . The oyster immunity . Developmental & Comparative Immunology , 80 : 99 - 118 , https://doi.org/10.1016/j.dci.2017.05.025 https://doi.org/10.1016/j.dci.2017.05.025 .

Weinert S , Jabs S , Supanchart C et al . 2010 . Lysosomal pathology and osteopetrosis upon loss of H + -driven lysosomal Cl⁻ accumulation . Science , 328 ( 5984 ): 1401 - 1403 , https://doi.org/10.1126/science.1188072 https://doi.org/10.1126/science.1188072 .

Xiao S , Wong N K , Li J et al . 2018 . Analysis of in situ transcriptomes reveals divergent adaptive response to hyper- and hypo-salinity in the Hong Kong Oyster, Crassostrea hongkongensis . Frontiers in Physiology , 9 : 1491 , https://doi.org/10.3389/fphys.2018.01491 https://doi.org/10.3389/fphys.2018.01491 .

Yancey P H . 2005 . Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses . Journal of Experimental Biology , 208 ( 15 ): 2819 - 2830 , https://doi.org/10.1242/jeb.01730 https://doi.org/10.1242/jeb.01730 .

Zhang X Y , Mao F , Wong N K et al . 2020 . CLIC2α chloride channel orchestrates immunomodulation of hemocyte phagocytosis and bactericidal activity in Crassostrea gigas . iScience , 23 ( 7 ): 101328 , https://doi.‍org/10.1016/j.isci.2020.101328 https://doi.‍org/10.1016/j.isci.2020.101328 . https://do 10.1016/j.isci.2020.101328 http://dx.doi.org/10.1016/j.isci.2020.101328

Zhao C S , Wei W , Luo S W et al . 2021 . FABP regulates fatty acid metabolism and oxidative response via PPARα/RXR signaling in Litopenaeus vannamei following environmental exposure of clofibric acid . Ecotoxicology , 30 ( 5 ): 954 - 965 , https://doi.org/10.1007/s10646-021-02408-3 https://doi.org/10.1007/s10646-021-02408-3 .

Zhao X L , Yu H , Kong L F et al . 2012 . Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas . PLoS One , 7 ( 9 ): e46244 , https://doi.org/10.1371/journal.pone.0046244 https://doi.org/10.1371/journal.pone.0046244 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

Identification of suitable reference genes for quantitative gene expression analysis in clam Cyclina sinensis under salinity stress and Vibrio infection

Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

Effects of small peptides, probiotics, prebiotics, and synbiotics on growth performance, digestive enzymes, and oxidative stress in orange-spotted grouper, Epinephelus coioides, juveniles reared in artificial seawater

Multivariable Constrained Model Predictive Control ofAero-Engine Based on Augmented Predictive Model

Related Author

Zhicai SHE

Youhou XU

Peng ZHU

Lingxin KONG

Zhen JIA

Qiong DENG

Ziao CHEN

Yeshao PENG

Related Institution

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf Marine Ecological Environment Field Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University

College of Marine Technology and Environment, Dalian Ocean University

College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University

Innovation Base for Chinese Perch Breeding, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture

College of Marine and Biological Engineering, Yancheng Institute of Technology

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

DOI：

摘要

Abstract

关键词

Keywords

references