Effect of <italic style="font-style: italic">ChREBP</italic> on carbohydrate feeding regulation in mandarin fish <italic style="font-style: italic">Siniperca chuatsi</italic>

Jun YANG; Yulan KUANG; Xufang LIANG; Wenjing CAI; Jing XU; Junjie GAO; Junran WEI; Shan HE; Zengqiang YIN; Jinshui LIANG

Your Location：

Home >

Browse articles >

Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

Aquaculture and Fisheries | Updated：2024-10-14

- Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi
- Journal of Oceanology and Limnology Vol. 41, Issue 5, Pages: 1988-1996(2023)
- Affiliations：
  
  1. College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
  2. College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China
  3. Innovation Base for Chinese Perch Breeding, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China
- Author bio：
  
  ** xfliang@mail.hzau.edu.cn
- Funds：
- DOI：
  CLC：
- Received：22 November 2021，
  
  Revised：2022-02-08，
  
  Accepted：27 May 2022，
  
  Published：2023
- Accepted：
Scan QR Code
Jun YANG, Yulan KUANG, Xufang LIANG, et al. Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi[J]. Journal of Oceanology and Limnology, 2023, 41(5): 1988-1996.
DOI：

Jun YANG, Yulan KUANG, Xufang LIANG, et al. Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi[J]. Journal of Oceanology and Limnology, 2023, 41(5): 1988-1996. DOI：

摘要

Abstract

Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate. Therefore

the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish

the effective conversion and utilization of carbohydrate and lipids

and the feedback regulation of feeding are the key links for the efficient utilization of carnivorous fish feed. Carbohydrate response element binding protein (ChREBP) is a new transcription factor found in recent years in the glucose signaling pathway

and can also participate in feeding regulation. We performed in-vivo and in-vitro experiments to reveal the role of ChREBP in the glucose metabolism and feeding in mandarin fish. The mRNA expression of

ChREBP

and appetite regulatory factors were measured after intraperitoneal injection of glucose in mandarin fish

Siniperca chuatsi

and cotransfection with glucose and glucose+siRNA in the hypothalamic cells in mandarin fish. The results reveal that at hour 2 and 4 post intraperitoneal injection with 1 mg/g BW glucose

the blood glucose level of the mandarin fish increased significantly

but food intake decreased significantly

and it also displayed a significantly increased

ChREBP

mRNA expression levels in liver. At hour 4 post injection

hypothalamic

ChREBP

mRNA level was significantly increased

whereas the mRNA expressi

on levels of appetite genes neuropeptide Y (

npy

) and agouti-related peptide (

AgRP

) were decreased significantly. When the glucose concentration was 40 mmol/L

the expression level of

ChREBP

mRNA in mandarin fish hypothalamic cells was significantly up-regulated

but the expression level of appetite gene

npy

mRNA was down-regulated. When siRNA and glucose were co-transfected into mandarin fish brain cells

the expression level of chrebp mRNA was significantly decreased

and the appetite gene

npy

mRNA was significantly increased. The results indicated that glucose regulated food intake through the modulation of appetite gene

npy

by C

hREBP

关键词

Keywords

references

Cai W J , Liang X F , Yuan X C et al . 2018 . Different strategies of grass carp ( Ctenopharyngodon idella ) responding to insufficient or excessive dietary carbohydrate . Aquaculture , 497 : 292 - 298 , https://doi.org/10.1016/j.aquaculture.2018.07.042 https://doi.org/10.1016/j.aquaculture.2018.07.042 .

Delgado M J , Cerdá-Reverter J M , Soengas J L . 2017 . Hypothalamic integration of metabolic, endocrine, and circadian signals in fish: involvement in the control of food intake . Frontiers in Neuroscience , 11 : 354 , https://doi.org/10.3389/fnins.2017.00354 https://doi.org/10.3389/fnins.2017.00354 .

Dentin R , Benhamed F , Hainault I et al . 2006 . Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob / ob mice . Diabetes , 55 ( 8 ): 2159 - 2170 , https://doi.org/10.2337/db06-0200 https://doi.org/10.2337/db06-0200 .

Fu X , Li N , Lai Y et al . 2015 . A novel fish cell line derived from the brain of Chinese perch Siniperca chuatsi : development and characterization . Journal of Fish Biology , 86 ( 1 ): 32 - 45 , https://doi.org/10.1111/jfb.12540 https://doi.org/10.1111/jfb.12540 .

Fulci V , Macino G . 2007 . Quelling: post-transcriptional gene silencing guided by small RNAs in Neurospora crassa . Current Opinion in Microbiology , 10 ( 2 ): 199 - 203 , https://doi.org/10.1016/j.mib.2007.03.016 https://doi.org/10.1016/j.mib.2007.03.016 .

Hemre G I , Hansen T . 1998 . Utilisation of different dietary starch sources and tolerance to glucose loading in Atlan tic salmon ( Salmo salar ), during parr-smolt transformation . Aquaculture , 161 ( 1-4 ): 145 - 157 , https://doi.org/10.1016/S0044-8486(97)00266-4 https://doi.org/10.1016/S0044-8486(97)00266-4 .

Hemre G I , Mommsen T P , Krogdahl Å . 2002 . Carbohydrates in fish nutrition: effects on growth, glucose metabolism and hepatic enzymes . Aquaculture Nutrition , 8 ( 3 ): 175 - 194 , https://doi.org/10.1046/j.1365-2095.2002.00200.x https://doi.org/10.1046/j.1365-2095.2002.00200.x .

Hemre G I , Sandnes K , Lie Ø et al . 1995 . Carbohydrate nutrition in Atlantic salmon, Salmo salar L.: growth and feed utilization . Aquaculture Research , 26 ( 3 ): 149 - 154 , https://doi.org/10.1111/j.1365-2109.1995.tb00896.x https://doi.org/10.1111/j.1365-2109.1995.tb00896.x .

Hung S S O , Lutes P B , Conte F S et al . 1989 . Growth and feed efficiency of white sturgeon ( Acipenser transmontanus ) sub-yearlings at different feeding rates . Aquaculture , 80 ( 1-2 ): 147 - 153 , https://doi.org/10.1016/0044-8486(89)90280-9 https://doi.org/10.1016/0044-8486(89)90280-9 .

Iizuka K , Bruick R K , Liang G S et al . 2004 . Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis . Proceedings of the National Academy of Sciences of the United States of America , 101 ( 19 ): 7281 - 7286 , https://doi.org/10.1073/pnas.0401516101 https://doi.org/10.1073/pnas.0401516101 .

Iroz A , Montagner A , Benhamed F et al . 2017 . A specific ChREBP and PPARα cross-talk is required for the glucose-mediated FGF21 response . Cell Reports , 21 ( 2 ): 403 - 416 , https://doi.org/10.1016/j.celrep.2017.09.065 https://doi.org/10.1016/j.celrep.2017.09.065 .

Jones L , Hamilton A J , Voinnet O et al . 1999 . RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing . The Plant Cell , 11 ( 12 ): 2291 - 2301 , https://doi.org/10.1105/tpc.11.12.2291 https://doi.org/10.1105/tpc.11.12.2291 .

Katz LS , Xu S , Ge K et al . 2018 . T3 and glucose coordinately stimulate ChREBP-mediated Ucp1 expression in brown adipocytes from male mice . Endocrinology , 159 ( 1 ): 557 - 569 , https://doi.org/10.1210/en.2017-00579 https://doi.org/10.1210/en.2017-00579 .

Kurokawa T , Murashita K . 2009 . Genomic characterization of multiple leptin genes and a leptin receptor gene in the Japanese medaka ( Oryzias latipes ) . General and Comparative Endocrinology , 161 ( 2 ): 229 - 237 , https://doi.org/10.1016/j.ygcen.2009.01.008 https://doi.org/10.1016/j.ygcen.2009.01.008 .

Martin R J , Xi X , Morrison C D . 2007 . In vivo and in vitro manipulation of AGRP expression and carbohydrate response element binding protein by nutrient status . Appetite , 49 ( 1 ): 311 , https://doi.org/10.1016/j.appet.2007.03.129 https://doi.org/10.1016/j.appet.2007.03.129 .

Meister G , Landthaler M , Dorsett Y et al . 2004 . Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing . RNA , 10 ( 3 ): 544 - 550 , https://doi.org/10.1261/rna.5235104 https://doi.org/10.1261/rna.5235104 .

Pan Z , Wang J , Kang B et al . 2010 . Screening and identification of differentially expressed genes in goose hepatocytes exposed to free fatty acid . Journal of Cellular Biochemistry , 111 ( 6 ): 1482 - 1492 , https://doi.org/10.1002/jcb.22878 https://doi.org/10.1002/jcb.22878 .

Peng Y . 2009 . The Role of ChREBP and ACC1 Gene in Glyco- and Lipometabolism in Hepatocyte . Huazhong Agricultural University , Wuhan, China . (in Chinese with English abstract)

Prathibha Y , Senthilkumaran B . 2016 . Involvement of pax2 in ovarian development and recrudescence of cat fish: a role in steroidogenesis . Journal of Endocrinology , 231 ( 3 ): 181 - 195 , https://doi.org/10.1530/JOE-16-0103 https://doi.org/10.1530/JOE-16-0103 .

Shi L J , Liang X F , He S et al . 2020 . Primary culture and identification of brain neurons in Chinese perch ( Siniperca chuatsi ) . Journal of Fisheries of China , 44 ( 1 ): 43 - 48 . (in Chinese with English abstract)

Vandesompele J , De Preter K , Pattyn F et al . 2002 . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes . Genome Biol ogy , 3 ( 7 ): research0034.1 .

Wang L , Zhou J Y , Yao J H et al . 2010 . U6 promoter-driven siRNA injection has nonspecific effects in zebra fish . Biochemical and Biophysical Research Communications , 391 ( 3 ): 1363 - 1368 , https://doi.org/10.1016/j.bbrc.2009.12.065 https://doi.org/10.1016/j.bbrc.2009.12.065 .

Wang Y , Botolin D , Xu J H et al . 2006 . Regulation of hepatic fatty acid elongase and desaturase expression in diabetes and obesity . Journal of Lipid Research , 47 ( 9 ): 2028 - 2041 , https://doi.org/10.1194/jlr.M600177-JLR200 https://doi.org/10.1194/jlr.M600177-JLR200 .

Yamashita H , Takenoshita M , Sakurai M et al . 2001 . A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver . Proceedings of the National Academy of Sciences of the United States of America , 98 ( 16 ): 9116 - 9121 , https://doi.org/10.1073/pnas.161284298 https://doi.org/10.1073/pnas.161284298 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

Identification of potential sex-related genes inSinipercachuatsi

Study on kinetics of glucose uptake by some species of plankton

Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody

Crack Growth Rate Model Based on Modal Frequencyfor a V-Notched Beam

Related Author

Zhicai SHE

Youhou XU

Peng ZHU

Lingxin KONG

Zhen JIA

Qiong DENG

Ziao CHEN

Yeshao PENG

Related Institution

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Pinglu Canal and Beibu Gulf Coastal Ecosystem Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai), School of Life Sciences, Sun Yat-Sen University

Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources

Guangdong Liangshi Aquatic Seed Industry Co., Ltd.

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

DOI：

摘要

Abstract

关键词

Keywords

references