Morphological and molecular analyses of a <italic style="font-style: italic">Philine</italic> <italic style="font-style: italic">kinglipini</italic> outbreak in summer of 2022 in Jiaozhou Bay, China

Zelin DUAN; Yanan YU; Jing WANG; Junlong ZHANG; Nansheng CHEN

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Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay, China

Ecology | Updated：2024-10-14

- Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay, China
- Journal of Oceanology and Limnology Vol. 42, Issue 3, Pages: 831-848(2024)
- Affiliations：
  
  1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao 266237, China
  3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  4.Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  5.Marine Biological Museum, Chinese Academy of Sciences, Qingdao 266071, China
  6.Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
- Author bio：
  
  chenn@qdio.ac.cn
- Funds：
- DOI：
  CLC：
- Received：07 March 2023，
  
  Accepted：08 August 2023，
  
  Online First：18 September 2023，
  
  Published：01 May 2024
- Accepted：
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DUAN Zelin,YU Yanan,WANG Jing,et al.Morphological and molecular analyses of a Philinekinglipini outbreak in summer of 2022 in Jiaozhou Bay, China[J].Journal of Oceanology and Limnology,2024,42(03):831-848.
DOI：

DUAN Zelin,YU Yanan,WANG Jing,et al.Morphological and molecular analyses of a Philinekinglipini outbreak in summer of 2022 in Jiaozhou Bay, China[J].Journal of Oceanology and Limnology,2024,42(03):831-848. DOI：

摘要

Abstract

A sudden and unprecedented outbreak of molluscan

Philine

kinglipini

occurred in summer 2022 in Jiaozhou Bay

Shandong

China

causing substantial damage to local mariculture industry of the Manila clam

Ruditapes

philippinarum

. Although

kinglipini

has been fo

und in many coastal regions of China

the molecular research of

kinglipini

has not been extensively studied

making it difficult to accurately identify and track

kinglipini

samples in field using molecular methods. Samples were collected during the outbreak and their morphological features and molecular sequences were analyzed. Results show that the causative species of the outbreak was

kinglipini

. The mitochondrial genome (mtDNA) of

kinglipini

was constructed for the first time

based on which phylogenetic analysis of the mtDNAs of

kinglipini

and related species in the order Cephalaspidea was carried out. As revealed by metabarcoding analysis of 18S rDNA V4

the seasonal change of

kinglipini

and closely related

Philine

species was striking with peaks between April and August. Therefore

metabarcoding analysis is applicable tool for monitoring the bloom development of

kinglipini

and related species. This study generated for the first time essential molecular marker sequences and mtDNA of

kinglipini

which provided a reference for future characterization and monitoring of its outbreaks and for phylogenetic analysis of

Philine

species.

关键词

Keywords

references

Antognazza C M , Britton J R , Potter C et al . 2019 . Environmental DNA as a non-invasive sampling tool to detect the spawning distribution of European anadromous shads ( Alosa spp .). Aquatic Conservation : Marine and Freshwater Ecosystems , 29 ( 1 ): 148 - 152 , https://doi.org/10.1002/aqc.3010 https://doi.org/10.1002/aqc.3010 .

Berry T E , Saunders B J , Coghlan M L et al . 2019 . Marine environmental DNA biomonitoring reveals seasonal patterns in biodiversity and identifies ecosystem responses to anomalous climatic events . PLoS Genetics , 15 ( 2 ): e1007943 , https://doi.org/10.1371/journal.pgen.1007943 https://doi.org/10.1371/journal.pgen.1007943 .

Boore J L , Brown W M . 1998 . Big trees from little genomes: mitochondrial gene order as a phylogenetic tool . Current Opinion in Genetics & Development , 8 ( 6 ): 668 - 674 , https://doi.org/10.1016/s0959-437x(98)80035-x https://doi.org/10.1016/s0959-437x(98)80035-x .

Callahan B J , McMurdie P J , Holmes S P . 2017 . Exact sequence variants should replace operational taxonomic units in marker-gene data analysis . The ISME Journal , 11 ( 12 ): 2639 - 2643 , https://doi.org/10.1038/ismej.2017.119 https://doi.org/10.1038/ismej.2017.119 .

Callahan B J , McMurdie P J , Rosen M J et al . 2016 . DADA2: High-resolution sample inference from Illumina amplicon data . Nature Methods , 13 ( 7 ): 581 - 583 , https://doi.org/10.1038/nMeth.3869 https://doi.org/10.1038/nMeth.3869 .

Capella-Gutiérrez S , Silla-Martínez J M , Gabaldón T . 2009 . trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses . Bioinformatics , 25 ( 15 ): 1972 - 1973 , https://doi.org/10.1093/bioinformatics/btp348 https://doi.org/10.1093/bioinformatics/btp348 .

Djurhuus A , Closek C J , Kelly R P et al . 2020 . Environmental DNA reveals seasonal shifts and potential interactions in a marine community . Nature Communications , 11 ( 1 ): 254 , https://doi.org/10.1038/s41467-019-14105-1 https://doi.org/10.1038/s41467-019-14105-1 .

Du F Y , Lin Q , Jia X P et al . 2011 . Analysis on the characteristics of macrobenthis community in the North-west Daya Bay of South China Bay in spring . Acta Ecologica Sinica , 31 ( 23 ): 7075 - 7085 . (in Chinese with English abstract)

Ellis M R , Clark Z S R , Treml E A et al . 2022 . Detecting marine pests using environmental DNA and biophysical models . Science of the Total Environment , 816 : 151666 , https://doi.org/10.1016/j.scitotenv.2021.151666 https://doi.org/10.1016/j.scitotenv.2021.151666 .

Ficetola G F , Miaud C , Pompanon F et al . 2008 . Species detection using environmental DNA from water samples . Biology Letters , 4 ( 4 ): 423 - 425 , https://doi.org/10.1098/rsbl.2008.0118 https://doi.org/10.1098/rsbl.2008.0118 .

Ghiselli F , Gomes-dos-Santos A , Adema C M et al . 2021 . Molluscan mitochondrial genomes break the rules . Philosophical Transactions of the Royal Society B : Biological Sciences , 376 ( 1825 ): 20200159 , https://doi.org/10.1098/rstb.2020.0159 https://doi.org/10.1098/rstb.2020.0159 .

Goldberg C S , Sepulveda A , Ray A et al . 2013 . Environmental DNA as a new method for early detection of New Zealand mudsnails ( Potamopyrgus antipodarum ) . Freshwater Science , 32 ( 3 ): 792 - 800 , https://doi.org/10.1899/13-046.1 https://doi.org/10.1899/13-046.1 .

Greiner S , Lehwark P , Bock R . 2019 . OrganellarGenomeDRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes . Nucleic Acids Research , 47 ( W1 ): W59 - W64 , https://doi.org/10.1093/nar/gkz238 https://doi.org/10.1093/nar/gkz238 .

Guo X R , Liu X B , Zhang Q T et al . 2019 . Characteristics of macrobenthos communities and status quo of Branchiostoma belcheri tsingtauense resources in Changli sea area, Hebei Province . Journal of Ecology and Rural Environment , 35 ( 8 ): 1034 - 1042 , https://doi.org/10.19741/j.issn.1673-4831.2018.0883. https://doi.org/10.19741/j.issn.1673-4831.2018.0883. (in Chinese with English abstract)

Hamatani I . 1961 . Notes on veligers of Japanese opisthobranchs ( 3 ). Publications of the Seto Marine Biological Laboratory , 9 ( 1 ): 67 - 79 , https://doi.org/10.5134/174661 https://doi.org/10.5134/174661 .

Høisæter T . 2009 . Distribution of marine, benthic, shell bearing gastropods along the Norwegian coast . Fauna Norvegica , 28 : 5 - 106 , https://doi.org/10.5324/fn.v28i0.563 https://doi.org/10.5324/fn.v28i0.563 .

Horikoshi M . 1967 . Reproduction, larval features and life history of Philine denticulata (J. Adams) (Mollusca-Tectibranchia) . Ophelia , 4 ( 1 ): 43 - 84 , https://doi.org/10.1080/00785326.1967.10409612 https://doi.org/10.1080/00785326.1967.10409612 .

Huang H L , Xu Q , Gibson K et al . 2021 . Molecular characterization of harmful algal blooms in the Bohai Sea using metabarcoding analysis . Harmful Algae , 106 : 102066 , https://doi.org/10.1016/j.hal.2021.102066 https://doi.org/10.1016/j.hal.2021.102066 .

Jin J J , Yu W B , Yang J B et al . 2020 . GetOrganelle: A fast and versatile toolkit for accurate de novo assembly of organelle genomes . Genome Biology , 21 ( 1 ): 241 , https://doi.org/10.1186/s13059-020-02154-5 https://doi.org/10.1186/s13059-020-02154-5 .

Katoh K , Standley D M . 2013 . MAFFT multiple sequence alignment software version 7: improvements in performance and usability . Molecular Biology and Evolution , 30 ( 4 ): 772 - 780 , https://doi.org/10.1093/molbev/mst010 https://doi.org/10.1093/molbev/mst010 .

Krzywinski M , Schein J , Birol I et al . 2009 . Circos: an information aesthetic for comparative genomics . Genome Research , 19 ( 9 ): 1639 - 1645 , https://doi.org/10.1101/gr.092759.109 https://doi.org/10.1101/gr.092759.109 .

Kurita Y , Kijima A . 2019 . A noninvasive method for extracting bivalve DNA from the water-filled mantle cavity . Hydrobiologia , 829 ( 1 ): 237 - 243 , https://doi.org/10.1007/s10750-018-3835-1 https://doi.org/10.1007/s10750-018-3835-1 .

Lagesen K , Hallin P , Rødland E A et al . 2007 . RNAmmer: consistent and rapid annotation of ribosomal RNA genes . Nucleic Acids Research , 35 ( 9 ): 3100 - 3108 , https://doi.org/10.1093/nar/gkm160 https://doi.org/10.1093/nar/gkm160 .

Lancaster S M . 1983 . The biology and reproductive ecology of Philine aperta (Opisthobranchia: Bullomorpha) in Oxwich Bay . Journal of Molluscan Studies , 49 ( supp12A ): 82 - 88 , https://doi.org/10.1093/oxfordjournals.mollus.a065771 https://doi.org/10.1093/oxfordjournals.mollus.a065771 .

Li B Q , Li X Z , Yu H Y et al . 2005 . Macrobenthic mollusca fauna and its relations to environmental factors in Jiaozhou Bay . Oceanologia et Limnologia Sinica , 36 ( 3 ): 193 - 198 , https://doi.org/10.3321/j.issn:0029-814X.2005.03.001. https://doi.org/10.3321/j.issn:0029-814X.2005.03.001. (in Chinese with English abstract)

Li H , Durbin R . 2009 . Fast and accurate short read alignment with Burrows-Wheeler transform . Bioinformatics , 25 ( 14 ): 1754 - 1760 , https://doi.org/10.1093/bioinformatics/btp324 https://doi.org/10.1093/bioinformatics/btp324 .

Li H , Handsaker B , Wysoker A et al . 2009 . The sequence alignment/map format and SAMtools . Bioinformatics , 25 ( 16 ): 2078 - 2079 , https://doi.org/10.1093/bioinformatics/btp352 https://doi.org/10.1093/bioinformatics/btp352 .

Li X Z , Wang H F , Zhang B L et al . 2012 . Advance of the study on the macrobenthos from the Yellow Sea and East China Sea . Sustainable Development , 2 ( 2 ): 80 - 102 , https://doi.org/10.12677/sd.2012.22013. https://doi.org/10.12677/sd.2012.22013. (in Chinese with English abstract)

Lin G Y . 1997 . Fauna Sinica. Mollusca, Gastropoda, Subclass Opisthobranchia, Order Cephalaspidea . Science Press , Beijing . (in Chinese)

Liu S Y , Cui Z M , Zhao Y F et al . 2022a . Composition and spatial-temporal dynamics of phytoplankton community shaped by environmental selection and interactions in the Jiaozhou Bay . Water Research , 218 : 118488 , https://doi.org/10.1016/j.watres.2022.118488 https://doi.org/10.1016/j.watres.2022.118488 .

Liu S Y , Gibson K , Cui Z M et al . 2020 . Metabarcoding analysis of harmful algal species in Jiaozhou Bay . Harmful Algae , 92 : 101772 , https://doi.org/10.1016/j.hal.2020.101772 https://doi.org/10.1016/j.hal.2020.101772 .

Liu Y M , Ma P Z , Zhang Z et al . 2022b . The new phylogenetic relationships in Veneridae (Bivalvia: Venerida) . Zoological Journal of the Linnean Society , 196 ( 1 ): 346 - 365 , https://doi.org/10.1093/zoolinnean/zlac047 https://doi.org/10.1093/zoolinnean/zlac047 .

Lowe T M , Chan P P . 2016 . tRNAscan-SE On-line: integrating search and context for analysis of transfer RNA genes . Nucleic Acids Research , 44 ( W1 ): W54 - W57 , https://doi.org/10.1093/nar/gkw413 https://doi.org/10.1093/nar/gkw413 .

Malaquias M A E , Ohnheiser L T , Oskars T R et al . 2017 . Diversity and systematics of philinid snails (Gastropoda: Cephalaspidea) in West Africa with remarks on the biogeography of the region . Zoological Journal of the Linnean Society , 180 : 1 - 35 , https://doi.org/10.1111/zoj.12478 https://doi.org/10.1111/zoj.12478 .

Marshall N T , Symonds D E , Dean C A et al . 2022 . Evaluating environmental DNA metabarcoding as a survey tool for unionid mussel assessments . Freshwater Biology , 67 ( 9 ): 1483 - 1507 , https://doi.org/10.1111/fwb.13955 https://doi.org/10.1111/fwb.13955 .

Minh B Q , Nguyen M A T , Von Haeseler A . 2013 . Ultrafast approximation for phylogenetic bootstrap . Molecular Biology and Evolution , 30 ( 5 ): 1188 - 1195 , https://doi.org/10.1093/molbev/mst024 https://doi.org/10.1093/molbev/mst024 .

Ohnheiser L T , Malaquias M . 2013 . Systematic revision of the gastropod family Philinidae (Mollusca: Cephalaspidea) in the north-east Atlantic Ocean with emphasis on the Scandinavian Peninsula . Zoological Journal of the Linnean Society , 167 ( 2 ): 273 - 326 , https://doi.org/10.1111/zoj.12000 https://doi.org/10.1111/zoj.12000 .

Price R M , Gosliner T M , Valdés Á . 2011 . Systematics and phylogeny of Philine (Gastropoda: Opisthobranchia), with emphasis on the Philine aperta species complex . The Veliger , 51 : 1 - 58 .

Rhodes K L . 1998 . Seasonal trends of Epibenthic Macrofaunal Assemblages in the Nearshore Waters of the Western Yellow Sea, Qingdao, People's Republic of China . Estuarine, Coastal and Shelf Science , 46 ( 5 ): 629 - 643 , https://doi.org/10.1006/ecss.1997.0292 https://doi.org/10.1006/ecss.1997.0292 .

Seager J R . 1979 . Reproductive biology of the Antarctic opisthobranch Philine gibba Strebel . Journal of Experimental Marine Biology and Ecology , 41 ( 1 ): 51 - 74 , https://doi.org/10.1016/0022-0981(79)90080-7 https://doi.org/10.1016/0022-0981(79)90080-7 .

Sigsgaard E E , Nielsen I B , Bach S S et al . 2016 . Population characteristics of a large whale shark aggregation inferred from seawater environmental DNA . Nature Ecology & Evolution , 1 ( 1 ): 0004 , https://doi.org/10.1038/s41559-016-0004 https://doi.org/10.1038/s41559-016-0004 .

Smith S A , Dunn C W . 2008 . Phyutility: a phyloinformatics tool for trees, alignments and molecular data . Bioinformatics , 24 ( 5 ): 715 - 716 , https://doi.org/10.1093/bioinformatics/btm619 https://doi.org/10.1093/bioinformatics/btm619 .

Sun S , Li Y H , Sun X X . 2012 . Changes in the small-jellyfish community in recent decades in Jiaozhou Bay, China . Chinese Journal of Oceanology and Limnology , 30 ( 4 ): 507 - 518 , https://doi.org/10.1007/s00343-012-1179-7 https://doi.org/10.1007/s00343-012-1179-7 .

Tang Q H , Lei N , Li J et al . 2015 . Seabed mixed sediment classification with multi-beam echo sounder backscatter data in Jiaozhou bay . Marine Georesources & Geotechnology , 33 ( 1 ): 1 - 11 , https://doi.org/10.1080/1064119X.2013.764557 https://doi.org/10.1080/1064119X.2013.764557 .

Tchang S . 1934 . Contribution à l'étude de Opistobranchea de la Côte de Tsingtao . Contributions from the Institute of Zoology, National Academy of Peiping , 2 ( 2 ): 1 - 148 . (in French)

Thorvaldsdóttir H , Robinson J T , Mesirov J P . 2013 . Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration . Briefings in Bioinformatics , 14 ( 2 ): 178 - 192 , https://doi.org/10.1093/bib/bbs017 https://doi.org/10.1093/bib/bbs017 .

Trifinopoulos J , Nguyen L T , von Haeseler A et al . 2016 . W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis . Nucleic Acids Research , 44 ( W1 ): W232 - W235 , https://doi.org/10.1093/nar/gkw256 https://doi.org/10.1093/nar/gkw256 .

Troth C R , Sweet M J , Nightingale J et al . 2021 . Seasonality, DNA degradation and spatial heterogeneity as drivers of eDNA detection dynamics . Science of the Total Environment , 768 : 144466 , https://doi.org/10.1016/j.scitotenv.2020.144466 https://doi.org/10.1016/j.scitotenv.2020.144466 .

Tu L L , Liu W X , Sui J X et al . 2018 . The community structure of macrobenthos in the southern coastal waters nearby the Shandong peninsula in summer . Progress in Fishery Sciences , 39 ( 1 ): 27 - 36 , https://doi.‍org/10.11758/yykxjz.20161123001. https://doi.‍org/10.11758/yykxjz.20161123001. (in Chinese with English abstract)

Valentini A , Taberlet P , Miaud C et al . 2016 . Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding . Molecular Ecology , 25 ( 4 ): 929 - 942 , https://doi.org/10.1111/mec.13428 https://doi.org/10.1111/mec.13428 .

Van der Linden J . 1995 . Philinidae dredged by the CANCAP expeditions (Gastropoda, Opisthobranchia) . Basteria , 59 ( 1-3 ): 65 - 83 .

Wan A Y , Zhang G T . 2012 . Annual occurrence of moon jellyfish Aurelia sp.1 in the Jiaozhou Bay and its impacts on zooplankton community . Oceanologia et Limnologia Sinica , 43 ( 3 ): 494 - 501 , https://doi.org/10.11693/hyhz201203014014. https://doi.org/10.11693/hyhz201203014014. (in Chinese with English abstract)

Wang P P , Zhang F , Sun S et al . 2020 . Experimental clearance rates of Aurelia coerulea ephyrae and medusae, and the predation impact on zooplankton in Jiaozhou Bay . Journal of Oceanology and Limnology , 38 ( 4 ): 1256 - 1269 , https://doi.org/10.1007/s00343-020-0024-7 https://doi.org/10.1007/s00343-020-0024-7 .

Wang R F , Zhang C L , Xu B D et al . 2018 . Feeding strategy and prey selectivity of Chelidonichthys spinosus during autumn in Haizhou Bay . Journal of Fishery Sciences of China , 25 ( 5 ): 1059 - 1070 , https://doi.org/10.3724/SP.J.1118.2018.17350. https://doi.org/10.3724/SP.J.1118.2018.17350. (in Chinese with English abstract)

Wang Y C , Chen Y , Wang J et al . 2021 . Mitochondrial genome of the harmful algal bloom species Odontella regia (Mediophyceae, Bacillariophyta) . Journal of Applied Phycology , 33 ( 2 ): 855 - 868 , https://doi.org/10.1007/s10811-020-02364-1 https://doi.org/10.1007/s10811-020-02364-1 .

Wang Y T , Sun S . 2015 . Population dynamics of Aurelia sp.1 ephyrae and medusae in Jiaozhou Bay, China . Hydrobiologia , 754 ( 1 ): 147 - 155 , https://doi.org/10.1007/s10750-014-2021-3 https://doi.org/10.1007/s10750-014-2021-3 .

Wang Z , Qu F Y , Sui J X et al . 2016 . Community structure and diversity of macrobenthos in the western waters of Liaodong Bay during summer . Marine Sciences , 40 ( 1 ): 40 - 47 , https://doi.org/10.11759/hykx20140826003. https://doi.org/10.11759/hykx20140826003. (in Chinese with English abstract)

You Z J . 2004 . Distribution and fauna of opisthobranchia along the Zhejiang coast . Chinese Journal of Zoology , 39 ( 4 ): 11 - 15 , https://doi.org/10.3969/j.issn.0250-3263.2004.04.003. https://doi.org/10.3969/j.issn.0250-3263.2004.04.003. (in Chinese with English abstract)

Zamani N P , Zuhdi M F , Madduppa H . 2022 . Environmental DNA biomonitoring reveals seasonal patterns in coral reef fish community structure . Environmental Biology of Fishes , 105 ( 8 ): 971 - 991 , https://doi.‍org/10.1007/s10641-022-01274-0 https://doi.‍org/10.1007/s10641-022-01274-0 . https://do 10.1007/s10641-022-01274-0 http://dx.doi.org/10.1007/s10641-022-01274-0

Zhang Z H , Zhang X L , Xu Z J et al . 2015 . Emergency countermeasures against marine disasters in Qingdao City on the basis of scenario analysis . Natural Hazards , 75 ( S2 ): 233 - 255 , https://doi.org/10.1007/s11069-014-1332-z https://doi.org/10.1007/s11069-014-1332-z .

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Related Institution

Department of Molecular Biology and Biochemistry, Simon Fraser University, University Drive8888, Burnaby, British Columbia

Center for Ocean Mega-Science, Chinese Academy of Sciences

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CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences (IOCAS)

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⁰

Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay, China

DOI：

摘要

Abstract

关键词

Keywords

references