Morphological comparison and molecular marker screening of three <italic style="font-style: italic">Skeletonema</italic> species found in Changjiang (Yangtze) River Basin

Jingwen HU; Yuxin YI; Zhengxin YANG; John Patrick KOCIOLEK; Pan YU; Quanxi WANG; Qingmin YOU

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Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang (Yangtze) River Basin

Biology | Updated：2024-10-14

- Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang (Yangtze) River Basin
- Journal of Oceanology and Limnology Vol. 42, Issue 3, Pages: 923-942(2024)
- Affiliations：
  
  1. College of Life Sciences, Shanghai Normal University, Shanghai 200235, China
  2. Museum of Natural History, University of Colorado, Boulder 80309, USA
  3. College of Environmental and Geographical Sciences, Shanghai Normal University, Shanghai 200235, China
- Author bio：
  
  wangqx@shnu.edu.cn
  youqm1117@shnu.edu.cn
- Funds：
- DOI：
  CLC：
- Received：13 April 2023，
  
  Accepted：06 July 2023，
  
  Online First：12 September 2023，
  
  Published：01 May 2024
- Accepted：
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HU Jingwen,YI Yuxin,YANG Zhengxin,et al.Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang (Yangtze) River Basin[J].Journal of Oceanology and Limnology,2024,42(03):923-942.
DOI：

HU Jingwen,YI Yuxin,YANG Zhengxin,et al.Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang (Yangtze) River Basin[J].Journal of Oceanology and Limnology,2024,42(03):923-942. DOI：

摘要

Abstract

In our recent investigations of diatom diversity

we studied three species

namely

Skeletonema

costatum

Skeletonema

subsalsum

and

Skeletonema

potamos

. Although they have been found frequently in Changjiang (Yangtze) River Basin

their morphological and molecular identification is difficult in taxonomy. Therefore

to i

ntegrate morphological and molecular biological approaches

we compared systematically their morphological characters and performed phylogenetic analysis. Twelve strains of

Skeletonema

were collected and isolated from Shanghai and Jiangsu

China

and their morphological characteristics were examined by light microscopy (LM) and the scanning electron microscopy (SEM). Based on morphological comparison

we determined that

potamos

is easy to distinguish from the other two species. The heavily silicified areolae

undulated or cleft distal ends of terminal fultoportula processes (TFPPs)

absence of basal pores of fultoportula processes (FPPs)

the root-like protrusions of FPPs

and no interlocking connection are the stable characteristics that can be used to identify

potamos

. However

there are only two features that can distinguish

costatum

from

subsalsum

namely the location of terminal rimoportulae (TRPs) and the distal shape of TFPPs. In addition

we amplified and sequenced nine common genetic markers from the strains

from which 101 sequences were obtained

constructed phylogenetic trees based on the nine genes and evaluated that seven genes can be used to identify

potamos

and revealed that

subsalsum

is the closest known relative of

costatum

and only ATP synthetase beta-subunit gene (

atp

B) is able to distinguish them from each other

which strongly support that it is an effective molecular marker for

Skeletonema

. This work provided a theoretical basis for the taxonomic study of

Skeletonema

关键词

Keywords

references

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Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang (Yangtze) River Basin

DOI：

摘要

Abstract

关键词

Keywords

references