Combined proteomic and metabolomic studies on the liver of Amur sturgeon <italic style="font-style: italic">Acipenser</italic><italic style="font-style: italic">schrenckii</italic> under titanium dioxide nanoparticle exposure

Zhou ZHOU; Ting LIU; Jie KONG; Zhenxing ZHAO; Junquan ZHU

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Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure

Aquaculture and Fisheries | Updated：2024-10-14

- Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure
- Journal of Oceanology and Limnology Vol. 42, Issue 3, Pages: 1001-1015(2024)
- Affiliations：
  
  1. Key Laboratory of Aquacultural Biotechnology, Ministry of Education, and Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315000, China
  2. Guizhou Fisheries Research Institute, Guizhou Academy of Agriculture Sciences, Guiyang 550000, China
- Author bio：
  
  zhujunquan999@163.com
- Funds：
- DOI：
  CLC：
- Received：20 July 2023，
  
  Accepted：28 August 2023，
  
  Online First：18 September 2023，
  
  Published：01 May 2024
- Accepted：
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ZHOU Zhou,LIU Ting,KONG Jie,et al.Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenser, schrenckii under titanium dioxide nanoparticle exposure[J].Journal of Oceanology and Limnology,2024,42(03):1001-1015.
DOI：

ZHOU Zhou,LIU Ting,KONG Jie,et al.Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenser, schrenckii under titanium dioxide nanoparticle exposure[J].Journal of Oceanology and Limnology,2024,42(03):1001-1015. DOI：

摘要

Abstract

Nanomaterials

particularly titanium dioxide nanoparticles (TiO

-NPs)

are extensively utilized across various industries. However

their environmental release has raised concerns regarding their potential ecological and environmental impacts. The reproductive toxicity of TiO

-NPs in fish species has attracted considerable attention

yet conflicting research outcomes have been reported. We investigated the effects of TiO

-NPs exposure on the liver of juvenile Amur sturgeon

Acipenser

schrenckii

using label-free proteomic and untargeted metabolomic analyses. The experiment included a control group and three groups exposed to different concentrations of TiO

-NPs (low

TL; medium

TM; high

TH). Compared to the control group

and 25 proteins and 35

and 158 metabolites were differentially expressed in the TH

and TL TiO

-NP-exposed groups

respectively. The differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia for Genes and Genomes (KEGG) pathways related to glycolysis and gluconeogenesis. Moreover

among

the 126 correlated proteins

the most enriched pathways were associated with endocytosis and protein processing in the endoplasmic reticulum. Notably

syringic acid was significantly downregulated across all three TiO

-NP-exposed groups. To obtain a comprehensive overview of the TiO

-NP-induced expression changes

a co-regulated network of proteins and metabolites associated with TiO

-NPs exposure was constructed. Exposure to TiO

-NPs led to enrichment and alteration of pathways related to immune responses

including endocytosis

protein processing in the endoplasmic reticulum

and peroxisome proliferator-activated receptor (PPAR) signaling. In conclusion

our findings indicate that exposure to TiO

-NPs might disrupt glucose metabolism and induce immune responses

thus contributing to our understanding of the environmental impacts of nanomaterials and highlighting the need for further research and development of potential mitigation strategies.

关键词

Keywords

references

Anagha T , Gupta S , Sahu N P et al . 2021 . Titanium dioxide nanoparticles alter reproductive and thyroid hormones of Labeo rohita females: Amelioration through vitamin E and folic acid . Aquaculture , 539 : 736633 , https://doi.org/10.1016/j.aquaculture.2021.736633 https://doi.org/10.1016/j.aquaculture.2021.736633 .

Ates M , Demir V , Adiguzel R et al . 2013 . Bioaccumulation, subacute toxicity, and tissue distribution of engineered titanium dioxide nanoparticles in goldfish ( Carassius auratus ) . Journal of Nanomaterials , 2013 : 460518 , https://doi.org/10.1155/2013/460518 https://doi.org/10.1155/2013/460518 .

Blanco E , Shen H F , Ferrari M . 2015 . Principles of nanoparticle design for overcoming biological barriers to drug delivery . Nature Biotechnology , 33 ( 9 ): 941 - 951 , https://doi.org/10.1038/nbt.3330 https://doi.org/10.1038/nbt.3330 .

Boya P , Kroemer G . 2008 . Lysosomal membrane permeabilization in cell death . Oncogene , 27 ( 50 ): 6434 - 6451 , https://doi.org/10.1038/onc.2008.310 https://doi.org/10.1038/onc.2008.310 .

Braakhuis H M , Gosens I , Heringa M B et al . 2021 . Mechanism of action of TiO 2 : recommendations to reduce uncertainties related to carcinogenic potential . Annual Review of Pharmacology and Toxicology , 61 : 203 - 223 , https://doi.org/10.1146/annurev-pharmtox-101419-100049 https://doi.org/10.1146/annurev-pharmtox-101419-100049 .

Carrera M , Piñeiro C , Martinez I . 2020 . Proteomic strategies to evaluate the impact of farming conditions on food quality and safety in aquaculture products . Foods (Basel, Switzerland) , 9 ( 8 ): 1050 , https://doi.‍org/10.3390/foods9081050 https://doi.‍org/10.3390/foods9081050 . https://do 10.3390/foods9081050 http://dx.doi.org/10.3390/foods9081050

Chen Z J , Han S , Zhang J H et al . 2021 . Metabolomics screening of serum biomarkers for occupational exposure of titanium dioxide nanoparticles . Nanotoxicology , 15 ( 6 ): 832 - 849 , https://doi.org/10.1080/17435390.2021.1921872 https://doi.org/10.1080/17435390.2021.1921872 .

Cheng H , Yan W , Wu Q et al . 2018 . Adverse reproductive performance in zebrafish with increased bioconcentration of microcystin-LR in the presence of titanium dioxide nanoparticles . Environmental Science : Nano , 4 : 1208 - 1217 , https://doi.org/10.1039/c8en00174j https://doi.org/10.1039/c8en00174j .

Choi J E , Kim S , Ahn J H et al . 2010 . Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish . Aquatic Toxicology , 100 ( 2 ): 151 - 159 , https://doi.org/10.1016/j.aquatox.2009.12.012 https://doi.org/10.1016/j.aquatox.2009.12.012 .

Contrepois K , Wu S , Moneghetti K J et al . 2020 . Molecular choreography of acute exercise . Cell , 181 ( 5 ): 1112 - 1130.e16 , https://doi.org/10.1016/j.cell.2020.04.043 https://doi.org/10.1016/j.cell.2020.04.043 .

Fang Q , Shi Q , Guo Y et al . 2016 . Enhanced bioconcentration of Bisphenol A in the presence of nano-TiO 2 can lead to adverse reproductive outcomes in zebrafish . Environmental Science and Technologyl. , 50 : 1005 - 1013 , https://doi.org/10.1021/acs.est.5b05024 https://doi.org/10.1021/acs.est.5b05024 .

Federici G , Shaw B J , Handy R D . 2007 . Toxicity of titanium dioxide nanoparticles to rainbow trout ( Oncorhynchus mykiss ): gill injury, oxidative stress, and other physiological effects . Aquatic Toxicology , 84 ( 4 ): 415 - 430 , https://doi.org/10.1016/j.aquatox.2007.07.009 https://doi.org/10.1016/j.aquatox.2007.07.009 .

Felix L C , Folkerts E J , He Y H et al . 2017 . Poly (acrylic acid)-coated titanium dioxide nanoparticle and ultraviolet light co-exposure has minimal effect on developing zebrafish ( Danio rerio ) . Environmental Science : Nano , 4 ( 3 ): 658 - 669 , https://doi.org/10.1039/C6EN00436A https://doi.org/10.1039/C6EN00436A .

Fernández M N , Muñoz-Olivas R , Luque-Garcia J L . 2019 . SILAC-based quantitative proteomics identifies size-dependent molecular mechanisms involved in silver nanoparticles-induced toxicity . Nanotoxicology , 13 ( 6 ): 812 - 826 , https://doi.org/10.1080/17435390.2019.1579374 https://doi.org/10.1080/17435390.2019.1579374 .

Forester S C , Waterhouse A L . 2008 . Identification of Cabernet Sauvignon anthocyanin gut microflora metabolites . Journal of Agricultural and Food Chemistry , 56 ( 19 ): 9299 - 9304 , https://doi.org/10.1021/jf801309n https://doi.org/10.1021/jf801309n .

Greven A C , Merk T , Karagöz F et al . 2016 . Polycarbonate and polystyrene nanoplastic particles act as stressors to the innate immune system of fathead minnow ( Pimephales promelas ) . Environmental Toxicology and Chemistry , 35 ( 12 ): 3093 - 3100 , https://doi.org/10.1002/etc.3501 https://doi.org/10.1002/etc.3501 .

Hasawi N A , Alkandari M F , Luqmani Y A . 2014 . Phosphofructokinase: a mediator of glycolytic flux in cancer progression . Critical Reviews in Oncology/Hematology , 92 ( 3 ): 312 - 321 , https://doi.org/10.1016/j.critrevonc.2014.05.007 https://doi.org/10.1016/j.critrevonc.2014.05.007 .

Hornung V , Bauernfeind F , Halle A et al . 2008 . Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization . Nature Immunology , 9 ( 8 ): 847 - 856 , https://doi.org/10.1038/ni.1631 https://doi.org/10.1038/ni.1631 .

Huang R , Xu F K . 2017 . Study on the acute toxicity of retenone to Lateolabrax japonicas and Chaeturichthys stigmatias Richardson . Transactions of Oceanology and Limnology , 1 : 96 - 101 , https://doi.org/10.13984/j.cnki.cn37-1141.2017.01.013 https://doi.org/10.13984/j.cnki.cn37-1141.2017.01.013 .

Huang X Z , Lan Y W , Liu Z K et al . 2018 . Salinity mediates the toxic effect of nano-TiO 2 on the juvenile olive flounder Paralichthys olivaceus . Science of the Total Environment , 640 - 641 : 726 - 735 , https://doi.‍org/10.1016/j.scitotenv.2018.05.350 https://doi.‍org/10.1016/j.scitotenv.2018.05.350 .

Isaacson T , Damasceno C , Saravanan R et al . 2006 . Sample extraction techniques for enhanced proteomic analysis of plant tissues . Nature Protocols , 1 : 769 - 774 , https://doi.org/10.1038/nprot.2006.102 https://doi.org/10.1038/nprot.2006.102 .

Keller A A , McFerran S , Lazareva A et al . 2013 . Global life cycle releases of engineered nanomaterials . Journal of Nanoparticle Research , 15 ( 6 ): 1692 , https://doi.org/10.1007/s11051-013-1692-4 https://doi.org/10.1007/s11051-013-1692-4 .

Kim I , Xu W J , Reed J C . 2008 . Cell death and endoplasmic reticulum stress: disease relevance and therapeutic opportunities . Nature Reviews Drug Discovery , 7 ( 12 ): 1013 - 1030 , https://doi.org/10.1038/nrd2755 https://doi.org/10.1038/nrd2755 .

Li N , Wang L Y , Shi F et al . 2021 . Silica nanoparticle induces pulmonary fibroblast transdifferentiation via macrophage route: potential mechanism revealed by proteomic analysis . Toxicology in Vitro , 76 : 105220 , https://doi.org/10.1016/j.tiv.2021.105220 https://doi.org/10.1016/j.tiv.2021.105220 .

Li R H , Han B Y , Zhang X et al . 2018 . The tissue expression analysis of the GeIL-8 gene and the response to Cu 2+ stress in Gymnocypris eckloni . Acta Ecologae Animalis Domastici , 39 ( 6 ): 21 - 27 , https://doi.org/10.3969/j.issn.1673-1182.2018.06.005. https://doi.org/10.3969/j.issn.1673-1182.2018.06.005. (in Chinese with English abstract)

Liao S T , Han C , Xu D Q et al . 2019 . 4-Octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to exert anti-inflammatory effects . Nature Communication , 10 ( 1 ): 5091 , https://doi.org/10.1038/s41467-019-13078-5 https://doi.org/10.1038/s41467-019-13078-5 .

Liberti M V , Dai Z W , Wardell S E et al . 2017 . A predictive model for selective targeting of the Warburg effect through GAPDH inhibition with a natural product . Cell Metabolism , 26 ( 4 ): 648 - 659.e8 , https://doi.org/10.1016/j.cmet.2017.08.017 https://doi.org/10.1016/j.cmet.2017.08.017 .

Lu Y F , Zhang Y , Deng Y F et al . 2016 . Uptake and accumulation of polystyrene microplastics in zebrafish ( Danio rerio ) and toxic effects in liver . Environmental Science & Technology , 50 ( 7 ): 4054 - 4060 , https://doi.‍org/10.1021/acs.est.6b00183 https://doi.‍org/10.1021/acs.est.6b00183 . https://do 10.1021/acs.est.6b00183 http://dx.doi.org/10.1021/acs.est.6b00183

Luo Z , Li Z Q , Xie Z et al . 2020 . Rethinking nano-TiO 2 safety: overview of toxic effects in humans and aquatic animals . Small , 16 ( 36 ): 2002019 , https://doi.org/10.1002/smll.202002019 https://doi.org/10.1002/smll.202002019 .

Ma X W , Wu Y Y , Jin S B et al . 2011 . Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment . ACS Nano , 5 ( 11 ): 8629 - 8639 , https://doi.org/10.1021/nn202155y https://doi.org/10.1021/nn202155y .

Martin S A M , Vilhelmsson O , Médale F et al . 2003 . Proteomic sensitivity to dietary manipulations in rainbow trout . Biochimica et Biophysica Acta , 1651 ( 1-2 ): 17 - 29 , https://doi.org/10.1016/s1570-9639(03)00231-0 https://doi.org/10.1016/s1570-9639(03)00231-0 .

Maurer M M , Donohoe G C , Maleki H et al . 2016 . Comparative plasma proteomic studies of pulmonary TiO 2 nanoparticle exposure in rats using liquid chromatogr aphy tandem mass spectrometry . Journal of Proteomics , 130 : 85 - 93 , https://doi.org/10.1016/j.jprot.2015.09.010 https://doi.org/10.1016/j.jprot.2015.09.010 .

Oakes S A , Papa F R . 2015 . The role of endoplasmic reticulum stress in human pathology . Annual Review of Pathology : Mechanisms of Disease , 10 : 173 - 194 , https://doi.org/10.1146/annurev-pathol-012513-104649 https://doi.org/10.1146/annurev-pathol-012513-104649 .

Oberdörster G , Oberdörster E , Oberdörster J . 2005 . Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles . Environmental Health Perspectives , 113 ( 7 ): 823 - 839 , https://doi.org/10.1289/ehp.7339 https://doi.org/10.1289/ehp.7339 .

Olivares-Rubio H F , Vega-López A . 2016 . Fatty acid metabolism in fish species as a biomarker for environmental monitoring . Environmental Pollution , 218 : 297 - 312 , https://doi.org/10.1016/j.envpol.2016.07.005 https://doi.org/10.1016/j.envpol.2016.07.005 .

Ozcan L , Tabas I . 2012 . Role of endoplasmic reticulum stress in metabolic disease and other disorders . Annual Review of Medicine , 63 : 317 - 328 , https://doi.org/10.1146/annurev-med-043010-144749 https://doi.org/10.1146/annurev-med-043010-144749 .

Pang M X , Wang Y , Tang Y J et al . 2021 . Transcriptome sequencing and metabolite analysis reveal the toxic effects of nanoplastics on tilapia after exposure to polystyrene . Environmental Pollution , 277 : 116860 , https://doi.org/10.1016/j.envpol.2021.116860 https://doi.org/10.1016/j.envpol.2021.116860 .

Pegoraro C , Maczkowiak F , Monsoro-Burq A H . 2013 . Pfkfb (6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase) isoforms display a tissue-specific and dynamic expression during Xenopus laevis development . Gene Expression Patterns , 13 ( 7 ): 203 - 211 , https://doi.org/10.1016/j.gep.2013.04.002 https://doi.org/10.1016/j.gep.2013.04.002 .

Planchon M , Léger T , Spalla O et al . 2017 . Metabolomic and proteomic investigations of impacts of titanium dioxide nanoparticles on Escherichia coli . PLoS One , 12 ( 6 ): e0178437 , https://doi.org/10.1371/journal.pone.0178437 https://doi.org/10.1371/journal.pone.0178437 .

Rajapakse K , Drobne D , Kastelec D et al . 2016 . Proteomic analyses of early response of unicellular eukaryotic microorganism Tetrahymena thermophila exposed to TiO 2 particles . Nanotoxicology , 10 ( 5 ): 542 - 556 , https://doi.org/10.3109/17435390.2015.1091107 https://doi.org/10.3109/17435390.2015.1091107 .

Rashid H O , Yadav R K , Kim H R et al . 2015 . ER stress: autophagy induction, inhibition and selection . Autophagy , 11 ( 11 ): 1956 - 1977 , https://doi.org/10.1080/15548627.2015.1091141 https://doi.org/10.1080/15548627.2015.1091141 .

Rejman J , Oberle V , Zuhorn I S et al . 2004 . Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis . Biochemical Journal , 377 ( 1 ): 159 - 169 , https://doi.org/10.1042/bj20031253 https://doi.org/10.1042/bj20031253 .

Ren X , Zhao X , Duan X et al . 2017 . Enhanced bioconcentration of trisIJ(1,3-dichloro-2-propyl) phosphate in the presence of nano-TiO 2 can lead to adverse reproductive outcomes in zebrafish . Environmental Pollution , 233 : 612 - 622 , https://doi.org/10.1016/j.envpol.2017.10.101 https://doi.org/10.1016/j.envpol.2017.10.101 .

Saha S , Xiong X H , Chakraborty P K et al . 2016 . Gold nanoparticle reprograms pancreatic tumor microenvironment and inhibits tumor growth . ACS Nano , 10 ( 12 ): 10636 - 10651 , https://doi.org/10.1021/acsnano.6b02231 https://doi.org/10.1021/acsnano.6b02231 .

Salehi H , Chehregani A , Lucini L et al . 2018 . Morphological, proteomic and metabolomic insight into the effect of cerium dioxide nanoparticles to Phaseolus vulgaris L. under soil or foliar application . Science of the Total Environment , 616 - 617 : 1540 - 1551 , https://doi.org/10.1016/j.scitotenv.2017.10.159 https://doi.org/10.1016/j.scitotenv.2017.10.159 .

Souza I D C , Mendes V A S , Duarte I D et al . 2019 . Nanoparticle transport and sequestration: intracellular titanium dioxide nanoparticles in a neotropical fish . Science of the Total Environment , 658 : 798 - 808 , https://doi.org/10.1016/j.scitotenv.2018.12.142 https://doi.org/10.1016/j.scitotenv.2018.12.142 .

Tang T L , Zhang Z , Zhu X P . 2019 . Toxic effects of TiO 2 NPs on zebrafish . International Journal of Environmental Research and Public Health , 16 ( 4 ): 523 , https://doi.org/10.3390/ijerph16040523 https://doi.org/10.3390/ijerph16040523 .

Tanner L B , Goglia A G , Wei M H et al . 2018 . Four key steps control glycolytic flux in mammalian cells . Cell Systems , 7 ( 1 ): 49 - 62.e8 , https://doi.org/10.1016/j.cels.2018.06.003 https://doi.org/10.1016/j.cels.2018.06.003 .

Tsai S M , Bangalore P , Chen E Y et al . 2017 . Graphene-induced apoptosis in lung epithelial cells through EGFR . Journal of Nanoparticle Research , 19 ( 7 ): 262 , https://doi.org/10.1007/s11051-017-3957-9 https://doi.org/10.1007/s11051-017-3957-9 .

Turan N B , Erkan H S , Engin G O et al . 2019 . Nanoparticles in the aquatic environment: usage, properties, transformation and toxicity—a review . Process Safety and Environmental Protection , 130 : 238 - 249 , https://doi.org/10.1016/j.psep.2019.08.014 https://doi.org/10.1016/j.psep.2019.08.014 .

Ungewickell E , Ungewickell H , Holstein S E H et al . 1995 . Role of auxilin in uncoating clathrin-coated vesicles . Nature , 378 ( 6557 ): 632 - 635 , https://doi.org/10.1038/378632a0 https://doi.org/10.1038/378632a0 .

Wang J , Xu W J , Wang X W et al . 2019a . The role of endoplasmic reticulum in introducing nano-toxicology and the mechanism involved . Progress in Biochemistry and Biophysics , 46 ( 1 ): 20 - 31 , https://doi.org/10.16476/j.pibb.2018.0052. https://doi.org/10.16476/j.pibb.2018.0052. (in Chinese with English abstract)

Wang Z Y , Dong C F . 2019b . Gluconeogenesis in cancer: function and regulation of PEPCK, FBPase, and G6Pase . Trends in Cancer , 5 ( 1 ): 30 - 45 , https://doi.org/10.1016/j.trecan.2018.11.003 https://doi.org/10.1016/j.trecan.2018.11.003 .

Winkler H C , Notter T , Meyer U et al . 2018 . Critical review of the safety assessment of titanium dioxide additives in food . Journal of Nanobiotechnology , 16 ( 1 ): 51 , https://doi.org/10.1186/s12951-018-0376-8 https://doi.org/10.1186/s12951-018-0376-8 .

Wiśniewski J R , Zougman A , Nagaraj N et al . 2009 . Universal sample preparation method for proteome analysis . Nature Methods , 6 ( 5 ): 359 - 362 , https://doi.org/10.1038/nmeth.1322 https://doi.org/10.1038/nmeth.1322 .

Yan M J , Hou Y H , Lv S S et al . 2016 . Protein degradation related to endoplasmic reticulum stress . Progress in Biochemistry and Biophysics , 43 ( 6 ): 539 - 549 , https://doi.org/10.16476/j.pibb.2015.0350. https://doi.org/10.16476/j.pibb.2015.0350. (in Chinese with English abstract)

Yeo M K , Kang M . 2012 . The biological toxicities of two crystalline phases and differential sizes of TiO 2 nanoparticles during zebrafish embryogenesis development . Molecular & Cellular Toxicology , 8 ( 4 ): 317 - 326 , https://doi.org/10.1007/s13273-012-0039-z https://doi.org/10.1007/s13273-012-0039-z .

Yıldırım H K , Akçay Y D , Güvenç U et al . 2004 . Protection capacity against low-density lipoprotein oxidation and antioxidant potential of some organic and non-organic wines . International Journal of Food Sciences and Nutrition , 55 ( 5 ): 351 - 362 , https://doi.org/10.1080/09637480412331319781 https://doi.org/10.1080/09637480412331319781 .

Zhang M , Song X X , Lv K et al . 2017 . Differential proteomic analysis revealing the ovule abortion in the female-sterile line of Pinus tabulaeformis Carr . Plant Science , 260 : 31 - 49 , https://doi.org/10.1016/j.plantsci.2017.03.001 https://doi.org/10.1016/j.plantsci.2017.03.001 .

Zhang N , Zhang L R , Zhao L et al . 2017 . iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat . Scientific Reports , 7 ( 1 ): 7524 , https://doi.org/10.1038/s41598-017-08069-9 https://doi.org/10.1038/s41598-017-08069-9 .

Zhang R , Piao M J , Kim K C et al . 2012 . Endoplasmic reticulum stress signaling is involved in silver nanoparticles-induced apoptosis . International Journal of Biochemistry & Cell Biology , 44 ( 1 ): 224 - 232 , https://doi.org/10.1016/j.biocel.2011.10.019 https://doi.org/10.1016/j.biocel.2011.10.019 .

Zhao L , Zhu Y F , Chen Z J et al . 2018 . Cardiopulmonary effects induced by occupational exposure to titanium dioxide nanoparticles . Nanotoxicology , 12 ( 2 ): 169 - 184 , https://doi.org/10.1080/17435390.2018.1425502 https://doi.org/10.1080/17435390.2018.1425502 .

Zhou W C , Li X Y , Wang Y M et al . 2021 . Physiological and transcriptomic changes of zebrafish ( Danio rerio ) embryos-larvae in response to 2-MIB exposure . Journal of Hazardous Materials , 416 : 126142 , https://doi.org/10.1016/j.jhazmat.2021.126142 https://doi.org/10.1016/j.jhazmat.2021.126142 .

Zhu X S , Wang J X , Zhang X Z et al . 2010 . Trophic transfer of TiO 2 nanoparticles from Daphnia to zebrafish in a simplified freshwater food chain . Chemosphere , 79 ( 9 ): 928 - 933 , https://doi.org/10.1016/j.chemosphere.2010.03.022 https://doi.org/10.1016/j.chemosphere.2010.03.022 .

Zhu Y , Wu X Y , Liu Y X et al . 2020 . Integration of transcriptomics and metabolomics reveals the responses of earthworms to the long-term exposure of TiO 2 nanoparticles in soil . Science of the Total Environment , 719 : 137492 , https://doi.org/10.1016/j.scitotenv.2020.137492 https://doi.org/10.1016/j.scitotenv.2020.137492 .

Zhu Y , Zhang Y K , Li Y B et al . 2022 . Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes . Journal of Hazardous Materials , 434 : 128820 , https://doi.org/10.1016/j.jhazmat.2022.128820 https://doi.org/10.1016/j.jhazmat.2022.128820 .

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Zhou ZHOU

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Key Laboratory of Aquacultural Biotechnology Ministry of Education, and Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University

Department of Biology, School of Life Sciences, Anhui Agricultural University

Department of Cell Biology, School of Biology and Basic Medical Sciences, Soochow University

Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University

Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University

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Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure

DOI：

摘要

Abstract

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