Detoxification strategy of <italic style="font-style: italic">Microcystis aeruginosa</italic> to the toxicity of Cd(II): role of EPS in alleviating toxicity

Xingye HAN; Fangyuan LIU; Yibo ZHANG; Kai CHENG; Heyun WANG; Hongmei GE

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Detoxification strategy of Microcystis aeruginosa to the toxicity of Cd(II): role of EPS in alleviating toxicity

Ecology | Updated：2024-10-14

- Detoxification strategy of Microcystis aeruginosa to the toxicity of Cd(II): role of EPS in alleviating toxicity
- Journal of Oceanology and Limnology Vol. 42, Issue 3, Pages: 802-815(2024)
- Affiliations：
  
  1.Innovation Demonstration Base of Ecological Environment Geotechnical and Ecological Restoration of Rivers and Lakes, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, China
  2.School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
  3.National Engineering Research Center of Advanced Technology and Equipment for Water Environment Pollution Monitoring, Wuhan 430068, China
- Author bio：
  
  gehongmei@hbut.edu.cn
- Funds：
- DOI：
  CLC：
- Received：27 March 2023，
  
  Accepted：17 May 2023，
  
  Online First：03 July 2023，
  
  Published：01 May 2024
- Accepted：
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HAN Xingye,LIU Fangyuan,ZHANG Yibo,et al.Detoxification strategy of Microcystis aeruginosa to the toxicity of Cd(II): role of EPS in alleviating toxicity[J].Journal of Oceanology and Limnology,2024,42(03):802-815.
DOI：

HAN Xingye,LIU Fangyuan,ZHANG Yibo,et al.Detoxification strategy of Microcystis aeruginosa to the toxicity of Cd(II): role of EPS in alleviating toxicity[J].Journal of Oceanology and Limnology,2024,42(03):802-815. DOI：

摘要

Abstract

Although many studies have found that cadmium (Cd) can be toxic to microalgae

only a few reports focused on the role of extracellular polymeric substances (EPS) in Cd(II) detoxification. The biochemical and physiological endpoints of

Microcystis aeruginosa

including the composition and functional groups of soluble EPS (SL-EPS)

loosely bound EPS (LB-EPS)

and tightly bound EPS (TB-EPS)

were detected to elucidate the toxicity and detoxification mechanisms of Cd(II) for cyanobacteria. Toxicological and physiological assays on

aeruginosa

showed that the 0.25-mg/L Cd(II) resulted in a larger inhibition on growth and

. Nevertheless

Cd(II) significantly induced much higher contents of superoxide dismutase (SOD)

intracellular microcystin LR (MC-LR)

extracellular MC-LR

and EPS. Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(II) was absorbed into the EPS layer. Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(II) of algae biomass

SL-EPS

LB-EPS

and TB-EPS were somewhat different. The C=O/C=N groups of δ-lactam or protein were their prominent functional groups

suggesting that amide or

proteins in the EPS played a key role in the adsorption in Cd(II). The concentration of 0.25 mg/L of Cd(II) may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan. This study indicated that

aeruginosa

could detoxify Cd(II) stress via induction of antioxidant capacity (higher SOD activity and MC synthesis)

EPS production

and modification in chemical structure of EPS.

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references

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Detoxification strategy of Microcystis aeruginosa to the toxicity of Cd(II): role of EPS in alleviating toxicity

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Abstract

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