Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of <italic style="font-style: italic">Scapharca</italic><italic style="font-style: italic">broughtonii</italic>

Desheng ZOU; Weian CAO; Guilong LIU; Junhao NING; Xia LU; Jinjing WANG; Min CHEN; Bo LIU; Jinsheng ZHANG; Chunde WANG

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Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharcabroughtonii

Biology | Updated：2024-10-12

- Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharcabroughtonii
- Journal of Oceanology and Limnology Vol. 41, Issue 6, Pages: 2363-2373(2023)
- Affiliations：
  
  1. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  2. University of Chinese Academy of Sciences, Beijing 100049, China
  3. Yantai Spring-Sea AquaSeed Co., Ltd., Yantai 264006, China
  4. Qingdao Agricultural University, Qingdao 266109, China
  5. Zhaoyuan Xiadian Agricultural Technology Promotion Station, Yantai 265415, China
- Author bio：
  
  jhning@yic.ac.cn
- Funds：
- DOI：
  CLC：
- Received：11 March 2022，
  
  Accepted：16 June 2022，
  
  Online First：13 October 2022，
  
  Published：01 November 2023
- Accepted：
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ZOU Desheng,CAO Weian,LIU Guilong,et al.Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharca, broughtonii[J].Journal of Oceanology and Limnology,2023,41(06):2363-2373.
DOI：

ZOU Desheng,CAO Weian,LIU Guilong,et al.Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharca, broughtonii[J].Journal of Oceanology and Limnology,2023,41(06):2363-2373. DOI：

摘要

Abstract

Ocean warming is altering the habitats of marine invertebrates

which has resulted in an increased physiological stress to marine molluscs

especially those intertidal bivalves

such as the ark shell

Scapharca

broughtonii

. We investigated the physiological and transcriptional responses of ark shells to acute and chronic thermal stress results showed that at 33 °C

a significantly higher cumulative mortality (55.7%) occurred under acute thermal stress than chronic thermal stress. The apoptosis rate of hemocytes was sustained at higher levels and the necrosis rate was increased significantly in a time-dependent manner under acute thermal stress. However

under chronic thermal stress

the apoptosis

and necrosis rates exhibited similar change trends: a rapid increase followed by a gradual decline and sustained at a relatively high level until the end of the experiment. The expressions of heat shock protein genes (

HSP20

and

HSP90

)

apoptosis-related genes (

TRAF6

GRP78

NIX

and

Casp

)

antioxidative-related genes (

GST

and

MRP

) and cellular detoxification-related genes (

HbⅡB

NOS

and

ENO

) were up-regulated significantly under both acute and chronic thermal stresses. These results demonstrated that the anti-apoptotic system

antioxidant defense system

cellular detoxification system

and heat shock proteins (HSPs) played vital roles for ark shells in response to thermal stress. As acute thermal stress can result in irreversible damage to marine molluscs

it is thus advised that chronic thermal stress should be used to select thermal-resistant ark shell strains.

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Keywords

references

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Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharcabroughtonii

DOI：

摘要

Abstract

关键词

Keywords

references