<italic style="font-style: italic">Nannochloropsis</italic> artificial chromosomes (NannoACs) loom on the horizon

Li GUO; Guanpin YANG

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Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon

Biology | Updated：2024-10-12

- Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon
- Journal of Oceanology and Limnology Vol. 41, Issue 6, Pages: 2336-2347(2023)
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  3.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  yguanpin@ouc.edu.cn
- Funds：
- DOI：
  CLC：
- Received：30 August 2022，
  
  Accepted：13 October 2022，
  
  Online First：24 October 2022，
  
  Published：01 November 2023
- Accepted：
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GUO Li,YANG Guanpin.Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon[J].Journal of Oceanology and Limnology,2023,41(06):2336-2347.
DOI：

GUO Li,YANG Guanpin.Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon[J].Journal of Oceanology and Limnology,2023,41(06):2336-2347. DOI：

摘要

Abstract

Species in genus

Nannochloropsis

especially

oceanica

and

gaditana

have been evolving as the model microalgae for both application and theory studies. The position effect of genome

integration

the carrying capability limitation of integrative vectors and the instability of non-integrative vectors have hindered

Nannochloropsis

genetic modification with concatenate genes and extremely long DNA fragments. The molecular tools including genetic transformation

homologous recombination

gene edition

gene stacking and episome vectors for transient gene expression and diverse reporters and selection markers have been rapidly developing in

Nannochloropsis

species. The construction of animal and plant artificial chromosomes with “top down” strategy has set fine examples for the construction of

Nannochloropsis

artificial chromosomes (NannoACs). It seems that the methods and materials to set the foundation for constructing NannoACs are at hands. In this review

we outlined the current status of transgenes in

Nannochloropsis

species

summarized the limitations of both integrative and non-integrative vectors

and proposed a tentative approach to construct NannoACs by doubling and stabilizing the genome first

and then truncating the natural chromosomes. NannoACs once constructed will facilitate transferring the desired traits and concatenate genes into

Nannochloropsis

genetic backgrounds

thus contributing towards its genetic improvement and synthetic biological studies.

关键词

Keywords

references

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Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon

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