Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp <italic style="font-style: italic">Fenneropenaeus</italic><italic style="font-style: italic">chinensis</italic>

Jiaobing LI; Yan WANG; Jingjie HU; Yajin XU; Qingqian ZHOU; Lu ZHANG; Mengqiang WANG

Your Location：

Home >

Browse articles >

Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis

Biology | Updated：2024-10-14

- Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis
- Journal of Oceanology and Limnology Vol. 41, Issue 5, Pages: 1940-1953(2023)
- Affiliations：
  
  1. MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution (Sanya 572024), Ocean University of China, Qingdao 266071, China
  2. Laboratory for Marine Fisheries Science and Food Production Processes, and Center for Marine Molecular Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  3. Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China
- Author bio：
  
  wangmengqiang@ouc.edu.cn
- Funds：
- DOI：
  CLC：
- Received：08 July 2022，
  
  Accepted：07 August 2022，
  
  Online First：17 August 2022，
  
  Published：01 September 2023
- Accepted：
Scan QR Code
LI Jiaobing,WANG Yan,HU Jingjie,et al.Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeus, chinensis[J].Journal of Oceanology and Limnology,2023,41(05):1940-1953.
DOI：

LI Jiaobing,WANG Yan,HU Jingjie,et al.Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeus, chinensis[J].Journal of Oceanology and Limnology,2023,41(05):1940-1953. DOI：

摘要

Abstract

Glutathione S-transferases (GSTs) are a superfamily of multifun

ction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates. In the present study

a delta class GST gene (designated as FcδGST) was cloned from

Fenneropenaeus

chinensis

using rapid amplification of cDNA ends (RACE) technology. The complete cDNA sequence of FcδGST was 780 bp in length

which includes a 27-bp 5′ non-coding region (UTR)

a 117-bp 3′ UTR

a 636-bp open reading frame (ORF)

and a polyadenylate signal site (AATAAA) presented at the upstream of poly A tail. The FcδGST gene encoded 211 amino acids peptide

including a GST_N domain and a GST_C domain

and exhibited high similarity with previously reported delta GSTs. The predicted molecular mass of FcδGST protein was 23.39 kDa

and its theoretical isoelectric point (pI) was 5.34. The FcδGST mRNA transcripts were ubiquitously expressed in all the tested tissues

with the highest expression level in hemocytes and hepatopancreas. During the stimulation of

Vibrio

anguillarum

or white spot syndrome virus (WSSV)

the mRNA expression of FcδGST in hemocytes and hepatopancreas revealed significant up-regulation. The purified recombinant FcδGST protein (designated as rFcδGST) exhibited specific catalytic activity against 1-chloro-2

4-dinitrobenzene (CDNB) substrate with relatively low stable enzymatic activities. These results indicated that FcδGST was a fragile but typical novel delta class GST member and potentially involved in the innate immune responses of

chinensis

关键词

Keywords

references

Arockiaraj J , Gnanam A J , Palanisamy R et al . 2014 . A cytosolic glutathione s-transferase, GST- theta from freshwater prawn Macrobrachium rosenbergii : molecular and biochemical properties . Gene , 546 ( 2 ): 437 - 442 , https://doi.org/10.1016/j.gene.2014.05.063 https://doi.org/10.1016/j.gene.2014.05.063 .

Benedetti S , Nuvoli B , Catalani S et al . 2015 . Reactive oxygen species a double-edged sword for mesothelioma . Oncotarget , 6 ( 19 ): 16848 - 16865 , https://doi.‍org/10.18632/oncotarget.4253 https://doi.‍org/10.18632/oncotarget.4253 . https://do 10.18632/oncotarget.4253 http://dx.doi.org/10.18632/oncotarget.4253

Blanchette B , Feng X , Singh B R . 2007 . Marine glutathione S -transferases . Marine Biotechnology , 9 ( 5 ): 513 - 542 , https://doi.org/10.1007/s10126-007-9034-0 https://doi.org/10.1007/s10126-007-9034-0 .

Buggé D M , Hégaret H , Wikfors G H et al . 2007 . Oxidative burst in hard clam ( Mercenaria mercenaria ) haemocytes . Fish & Shellfish Immunology , 23 ( 1 ): 188 - 196 , https://doi.org/10.1016/j.fsi.2006.10.006 https://doi.org/10.1016/j.fsi.2006.10.006 .

Chaijarasphong T , Munkongwongsiri N , Stentiford G D et al . 2021 . The shrimp microsporidian Enterocytozoon hepatopenaei (EHP): biology, pathology, diagnostics and control . Journal of Invertebrate Pathology , 186 : 107458 , https://doi.org/10.1016/j.jip.2020.107458 https://doi.org/10.1016/j.jip.2020.107458 .

Chen J H , Xiao S , Deng Y W et al . 2011 . Cloning of a novel glutathione S-transferase 3 ( GST3 ) gene and expressionanalysis in pearl oyster, Pinctada martensii . Fish & Shellfish Immunology , 31 ( 6 ): 823 - 830 , https://doi.org/10.1016/j.fsi.2011.07.023 https://doi.org/10.1016/j.fsi.2011.07.023 .

Contreras-Vergara C A , Harris-Valle C , Sotelo-Mundo R R et al . 2004 . A mu-class glutathione S -transferase from the marine shrimp Litopenaeus vannamei : molecular cloning and active-site structural modeling . Journal of Biochemical and Molecular Toxicology , 18 ( 5 ): 245 - 252 , https://doi.org/10.1002/jbt.20033 https://doi.org/10.1002/jbt.20033 .

Contreras-Vergara C A , Valenzuela-Soto E M , Arvizu-Flores A A et al . 2008 . Role of invariant tyrosines in a crustacean mu-class glutathione S-transferase from shrimp Litopenaeus vannamei : site-directed mutagenesis of Y7 and Y116 . Biochimie , 90 ( 6 ): 968 - 971 , https://doi.org/10.1016/j.biochi.2008.02.005 https://doi.org/10.1016/j.biochi.2008.02.005 .

Corona M , Robinson G E . 2006 . Genes of the antioxidant system of the Honey Bee: annotation and phylogeny . Insect Molecular Biology , 15 ( 5 ): 687 - 701 , https://doi.‍org/10.1111/j.1365-2583.2006.00695.x https://doi.‍org/10.1111/j.1365-2583.2006.00695.x . https://do 10.1111/j.1365-2583.2006.00695.x http://dx.doi.org/10.1111/j.1365-2583.2006.00695.x

Duan Y F , Liu P , Li J T et al . 2013 . Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge . Fish & Shellfish Immunology , 35 ( 3 ): 661 - 670 , https://doi.‍org/10.1016/j.fsi.2013.05.016 https://doi.‍org/10.1016/j.fsi.2013.05.016 . https://do 10.1016/j.fsi.2013.05.016 http://dx.doi.org/10.1016/j.fsi.2013.05.016

Gonçalves-Soares D , Zanette J , Yunes J S et al . 2012 . Expression and activity of glutathione S -transferases and catalase in the shrimp Litopenaeus vannamei inoculated with a toxic Microcystis aeruginosa strain . Marine Environmental Research , 75 : 54 - 61 , https://doi.org/10.1016/j.marenvres.2011.07.007 https://doi.org/10.1016/j.marenvres.2011.07.007 .

Hayes J D , Flanagan J U , Jowsey I R . 2005 . Glutathione transferases . Annual Review of Pharmacology and Toxicology , 45 : 51 - 88 , https://doi.‍org/10.1146/annurev.‍pharmtox.‍45.120403.095857 https://doi.‍org/10.1146/annurev.‍pharmtox.‍45.120403.095857 . https://do 10.1146/annurev.pharmtox.45.120403.095857 http://dx.doi.org/10.1146/annurev.pharmtox.45.120403.095857

He Y Y , Wang Q , Li J et al . 2022 . Comparative proteomic profiling in Chinese shrimp Fenneropenaeus chinensis under low pH stress . Fish & Shellfish Immunology , 120 : 526 - 535 , https://doi.org/10.1016/j.fsi.2021.12.032 https://doi.org/10.1016/j.fsi.2021.12.032 .

Hu F , Ye K , Tu X F et al . 2018 . Identification and expression profiles of twenty-six glutathione S -transferase genes from rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) . International Journal of Biological Macromolecules , 120 : 1063 - 1071 , https://doi.org/10.1016/j.ijbiomac.2018.08.185 https://doi.org/10.1016/j.ijbiomac.2018.08.185 .

Huang Y F , Xu Z B , Lin X Y et al . 2011 . Structure and expression of glutathione S -transferase genes from the midgut of the Common cutworm, Spodoptera litura (Noctuidae) and their response to xenobiotic compounds and bacteria . Journal of Insect Physiology , 57 ( 7 ): 1033 - 1044 , https://doi.org/10.1016/j.jinsphys.2011.05.001 https://doi.org/10.1016/j.jinsphys.2011.05.001 .

Hunaiti A A , Soud M . 2000 . Effect of lead concentration on the level of glutathione, glutathione S -transferase, reductase and peroxidase in human blood . Science of the Total Environment , 248 ( 1 ): 45 - 50 , https://doi.org/10.1016/s0048-9697(99)00548-3 https://doi.org/10.1016/s0048-9697(99)00548-3 .

Johansson M W , Keyser P , Sritunyalucksana K et al . 2000 . Crustacean haemocytes and haematopoiesis . Aquaculture , 191 ( 1-3 ): 45 - 52 , https://doi.‍org/10.1016/s0044-8486(00)00418-x https://doi.‍org/10.1016/s0044-8486(00)00418-x . https://do 10.1016/s0044-8486(00)00418-x http://dx.doi.org/10.1016/s0044-8486(00)00418-x

Lee S Y , Söderhäll K . 2002 . Early events in crustacean innate immunity . Fish & Shellfish Immunology , 12 ( 5 ): 421 - 437 , https://doi.org/10.1006/fsim.2002.0420 https://doi.org/10.1006/fsim.2002.0420 .

Lee Y M , Seo J S , Jung S O et al . 2006 . Molecular cloning and characterization of θ-class glutathione S -transferase (GST-T) from the hermaphroditic fish Rivulus marmoratus and biochemical comparisons with α-class glutathione S -transferase (GST-A) . Biochemical and Biophysical Research Communications , 346 ( 3 ): 1053 - 1061 , https://doi.‍org/10.1016/j.bbrc.2006.06.014 https://doi.‍org/10.1016/j.bbrc.2006.06.014 . https://do 10.1016/j.bbrc.2006.06.014 http://dx.doi.org/10.1016/j.bbrc.2006.06.014

Li C H , Su X R , Li Y et al . 2012 . Two classes of glutathione S-transferase genes with different response profiles to bacterial cha llenge in Venerupis philippinarum . Fish & Shellfish Immunology , 32 ( 1 ): 219 - 222 , https://doi.org/10.1016/j.fsi.2011.10.032 https://doi.org/10.1016/j.fsi.2011.10.032 .

Li F , Wu H F , Wang Q et al . 2015 . Glutathione S-transferase ( GST ) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms . Chinese Journal of Oceanology and Limnology , 33 ( 3 ): 705 - 713 , https://doi.org/10.1007/s00343-015-4127-5 https://doi.org/10.1007/s00343-015-4127-5 .

Li J T , Li W T , Zhang X M . 2019 . Lower-intensity net-chasing training facilitates the survival and growth of the Chinese shrimp Fenneropenaeus chinensis reared at high stocking densities . Aquaculture , 508 : 36 - 45 , https://doi.org/10.1016/j.aquaculture.2019.04.065 https://doi.org/10.1016/j.aquaculture.2019.04.065 .

Li X P , Luan S , Luo K et al . 2022 . Comparative transcriptomic analysis of Chinese shrimp Fenneropenaeus chinensis infected with white spot syndrome virus . Aquaculture Reports , 22 : 100986 , https://doi.org/10.1016/j.aqrep.2021.100986 https://doi.org/10.1016/j.aqrep.2021.100986 .

Li Y Q , Song X R , Wang W L et al . 2017 . The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus . Developmental & Comparative Immunology , 71 : 59 - 69 , https://doi.org/10.1016/j.dci.2017.01.024 https://doi.org/10.1016/j.dci.2017.01.024 .

Martindale J L , Holbrook N J . 2002 . Cellular response to oxidative stress: signaling for suicide and survival . Journal of Cellular Physiology , 192 ( 1 ): 1 - 15 , https://doi.org/10.1002/jcp.10119 https://doi.org/10.1002/jcp.10119 .

Ouaissi A , Ouaissi M , Sereno D . 2002 . Glutathione S -transferases and related proteins from pathogenic human parasites behave as immunomodulatory factors . Immunology Letters , 81 ( 3 ): 159 - 164 , https://doi.‍org/10.1016/s0165-2478(02)00035-4 https://doi.‍org/10.1016/s0165-2478(02)00035-4 . https://do 10.1016/s0165-2478(02)00035-4 http://dx.doi.org/10.1016/s0165-2478(02)00035-4

Park J C , Hagiwara A , Park H G et al . 2020 . The glutathione S -transferase genes in marine rotifers and copepods: identification of GSTs and applications for ecotoxicological studies . Marine Pollution Bulletin , 156 : 111080 , https://doi.org/10.1016/j.marpolbul.2020.111080 https://doi.org/10.1016/j.marpolbul.2020.111080 .

Park J C , Lee M C , Yoon D S et al . 2019 . Genome-wide identification and expression of the entire 52 glutathione S -transferase (GST) subfamily genes in the Cu 2+ -exposed marine copepods Tigriopus japonicus and Paracyclopina nana . Aquatic Toxicology , 209 : 56 - 69 , https://doi.org/10.1016/j.aquatox.2019.01.020 https://doi.org/10.1016/j.aquatox.2019.01.020 .

Parrilla-Taylor D P , Zenteno-Savín T , Magallón-Barajas F J . 2013 . Antioxidant enzyme activity in pacific whiteleg shrimp ( Litopenaeus vannamei ) in response to infection with white spot syndrome virus . Aquaculture , 380 - 383 : 41 - 46 , https://doi.org/10.1016/j.aquaculture.2012.11.031 https://doi.org/10.1016/j.aquaculture.2012.11.031 .

Rameshthangam P , Ramasamy P . 2006 . Antioxidant and membrane bound enzymes activity in WSSV-infected Penaeus monodon Fabricius . Aquaculture , 254 ( 1-4 ): 32 - 39 , https://doi.org/10.1016/j.aquaculture.2005.10.011 https://doi.org/10.1016/j.aquaculture.2005.10.011 .

Ren H L , Xu D D , Gopalakrishnan S et al . 2009a . Gene cloning of a sigma class glutathione S-transferase from abalone ( Haliotis diversicolor ) and expression analysis upon bacterial challenge . Developmental & Comparative Immunology , 33 ( 9 ): 980 - 990 , https://doi.org/10.1016/j.dci.2009.04.003 https://doi.org/10.1016/j.dci.2009.04.003 .

Ren Q , Sun R R , Zhao X F et al . 2009b . A selenium-dependent glutathione peroxidase (Se-GPx) and two glutathione S-transferases (GSTs) from Chinese shrimp ( Fenneropenaeus chinensis ) . Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology , 149 ( 4 ): 613 - 623 , https://doi.‍org/10.1016/j.cbpc.2009.01.007 https://doi.‍org/10.1016/j.cbpc.2009.01.007 .

Schmittgen T D , Livak K J . 2008 . Analyzing real-time PCR data by the comparative C T method . Nature Protocols , 3 ( 6 ): 1101 - 1108 , https://doi.org/10.1038/nprot.2008.73 https://doi.org/10.1038/nprot.2008.73 .

Schrader M , Fahimi H D . 2006 . Peroxisomes and oxidative stress . Biochimica et Biophysica Acta (BBA)-Molecular Cell Research , 1763 ( 12 ): 1755 - 1766 , https://doi.org/10.1016/j.bbamcr.2006.09.006 https://doi.org/10.1016/j.bbamcr.2006.09.006 .

Sha Y J , Wang L , Liu M et al . 2016 . Effects of lactic acid bacteria and the corresponding supernatant on the survival, growth performance, immune response and disease resistance of Litopenaeus vannamei . Aquaculture , 452 : 28 - 36 , https://doi.org/10.1016/j.aquaculture.2015.10.014 https://doi.org/10.1016/j.aquaculture.2015.10.014 .

Shao Y N , Lv Z M , Li C H et al . 2017 . Molecular cloning and functional characterization of theta class glutathione S-transferase from Apostichopus japonicus . Fish & Shellfish Immunology , 63 : 31 - 39 , https://doi.org/10.1016/j.fsi.‍2017.02.004 https://doi.org/10.1016/j.fsi.‍2017.02.004 .

Sheehan D , Meade G , Foley V M et al . 2001 . Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily . Biochemical Journal , 360 ( 1 ): 1 - 16 , https://doi.org/10.1042/bj3600001 https://doi.org/10.1042/bj3600001 .

Smith V J , Brown J H , Hauton C . 2003 . Immunostimulation in crustaceans: does it really protect against infection? Fish & Shellfish Immunology , 15 ( 1 ): 71 - 90 , https://doi.org/10.1016/s1050-4648(02)00140-7 https://doi.org/10.1016/s1050-4648(02)00140-7 .

Song L S , Wang L L , Zhang H et al . 2015 . The immune system and its modulation mechanism in scallop . Fish & Shellfish Immunology , 46 ( 1 ): 65 - 78 , https://doi.org/10.1016/j.fsi.2015.03.013 https://doi.org/10.1016/j.fsi.2015.03.013 .

Toranzo A E , Magariños B , Romalde J L . 2005 . A review of the main bacterial fish diseases in mariculture systems . Aquaculture , 246 ( 1-4 ): 37 - 61 , https://doi.org/10.1016/j.aquaculture.2005.01.002 https://doi.org/10.1016/j.aquaculture.2005.01.002 .

Umasuthan N , Revathy K S , Lee Y et al . 2012 . A novel molluscan sigma-like glutathione S-transferase from Manila clam, Ruditapes philippinarum : cloning, characterization and transcriptional profiling . Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology , 155 ( 4 ): 539 - 550 , https://doi.org/10.1016/j.cbpc.2012.01.001 https://doi.org/10.1016/j.cbpc.2012.01.001 .

Vodiasova E A , Meger Y V , Lantushenko A O . 2021 . Identification and characterization of the novel genes encoding glutathione S-transferases in Mytilus galloprovincialis . Comparative Biochemistry and Physiology Part D: Genomics and Proteomics , 40 : 100926 , https://doi.org/10.1016/j.cbd.2021.100926 https://doi.org/10.1016/j.cbd.2021.100926

Wan Q , Whang I , Lee J . 2008 . Molecular cloning and characterization of three sigma glutathione S -transferases from disk abalone ( Haliotis discus discus ) . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 151 ( 3 ): 257 - 267 , https://doi.‍org/10.1016/j.cbpb.2008.07.012 https://doi.‍org/10.1016/j.cbpb.2008.07.012 . https://do 10.1016/j.cbpb.2008.07.012 http://dx.doi.org/10.1016/j.cbpb.2008.07.012

Wang C Y , Zhao J M , Mu C K et al . 2013a . cDNA cloning and mRNA expre ssion of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis . Fish & Shellfish Immunology , 34 ( 2 ): 697 - 703 , https://doi.org/10.1016/j.fsi.2012.11.020 https://doi.org/10.1016/j.fsi.2012.11.020 .

Wang M , Wang B , Liu M et al . 2018a . Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene ( Ai GSTσ) from bay scallop Argopecten irradians . ISJ-Invertebrate Survival Journal , 15 ( 1 ): 19 - 30 . https://do 10.25431/1824-307X/ISJ.V15I1.19-30 http://dx.doi.org/10.25431/1824-307X/ISJ.V15I1.19-30

Wang M , Wang L , Ni D et al . 2018b . The mRNA expression profiles demonst rating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri . ISJ-Invertebrate Survival Journal , 15 ( 1 ): 302 - 315 . https://do 10.25431/1824-307X/ISJ.V15I1.302-315 http://dx.doi.org/10.25431/1824-307X/ISJ.V15I1.302-315

Wang M , Wang L , Zhou Z et al . 2013b . The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis . International Journal of Immunogenetics , 40 ( 3 ): 230 - 240 .

Wang M Q , Wang B J , Jiang K Y et al . 2018c . A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri . Fish & Shellfish Immunology , 72 : 282 - 290 , https://doi.org/10.1016/j.fsi.2017.11.010 https://doi.org/10.1016/j.fsi.2017.11.010 .

Wang M Q , Wang L L , Guo Y et al . 2014 . A high mobility group box 1 (HMGB1) gene from Chlamys farreri and the DNA-binding ability and pro-inflammatory activity of its recombinant protein . Fish & Shellfish Immunology , 36 ( 2 ): 393 - 400 , https://doi.org/10.1016/j.fsi.2013.12.008 https://doi.org/10.1016/j.fsi.2013.12.008 .

Wang M Q , Wang L L , Yi Q L et al . 2015 . Molecular cloning and characterization of a cytoplasmic manganese superoxide dismutase and a mitochondrial manganese superoxide dismutase from Chinese mitten crab Eriocheir sinensis . Fish & Shellfish Immunology , 47 ( 1 ): 407 - 417 , https://doi.org/10.1016/j.fsi.2015.09.035 https://doi.org/10.1016/j.fsi.2015.09.035 .

Wang M Q , Yang J L , Zhou Z et al . 2011 . A primitive Toll-like receptor signaling pathway in mollusk Zhikong scallop Chlamys farreri . Developmental & Comparative Immunology , 35 ( 4 ): 511 - 520 , https://doi.org/10.1016/j.dci.2010.12.005 https://doi.org/10.1016/j.dci.2010.12.005 .

Wang S , Ji C L , Li F et al . 2022 . Toxicological responses of juvenile Chinese shrimp Fenneropenaeus chinensis and swimming crab Portunus trituberculatus exposed to cadmium . Ecotoxicology and Environmental Safety , 234 : 113416 , https://doi.org/10.1016/j.ecoenv.2022.113416 https://doi.org/10.1016/j.ecoenv.2022.113416 .

Wang Y , Duan Y F , Wang J et al . 2019 . Identification and expression profile of Delta - GST in black tiger shrimp ( Penaeus monodon ) exposed to aflatoxin B1 (AFB1) . Journal of Experimental Zoology Part A : Ecological Genetics and Physiology , 331 ( 8 ): 443 - 455 , https://doi.org/10.1002/jez.2311 https://doi.org/10.1002/jez.2311 .

Wang Y , Liu L H , Huang J H et al . 2016 . Response of a Mu-class glutathione S -transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure . SpringerPlus , 5 ( 1 ): 825 , https://doi.org/10.1186/s40064-016-2381-4 https://doi.org/10.1186/s40064-016-2381-4 .

Xia Q , Wang B J , Liu M et al . 2015 . A new method to evaluate the effects of bacterial dosage, infection route and Vibrio strain in experimental challenges of Litopenaeus vannamei , based on the Cox proportional hazard model . Fish & Shellfish Immunology , 46 ( 2 ): 686 - 692 , https://doi.org/10.1016/j.fsi.2015.07.009 https://doi.org/10.1016/j.fsi.2015.07.009 .

Yamamoto K , Higashiura A , Hossain T et al . 2015 . Structural characterization of the catalytic site of a Nilaparvata lugens delta-class glutathione transferase . Archives of Biochemistry and Biophysics , 566 : 36 - 42 , https://doi.org/10.1016/j.abb.2014.12.001 https://doi.org/10.1016/j.abb.2014.12.001 .

Yang J L , Wei X M , Xu J et al . 2012 . A sigma-class glutathione S-transferase from Solen grandis that responded to microorganism glycan and organic contaminants . Fish & Shellfish Immunology , 32 ( 6 ): 1198 - 1204 , https://doi.org/10.1016/j.fsi.2012.03.010 https://doi.org/10.1016/j.fsi.2012.03.010 .

Yang T , Ren J S , Kooijman S A L M et al . 2020 . A dynamic energy budget model of Fenneropenaeus chinensis with applications for aquaculture and stock enhancement . Ecological Modelling , 431 : 109186 , https://doi.org/10.1016/j.ecolmodel.2020.109186 https://doi.org/10.1016/j.ecolmodel.2020.109186 .

Yi Q , Liu R , Sun R et al . 2014 . The protection of CpG ODNs and Yarrowia lipolytica harboring VP28 for shrimp Litopenaeus vannamei against white spot syndrome virus infection . ISJ-Invertebrate Survival Journal , 11 ( 1 ): 119 - 131 .

Zhang J K , Li F H , Jiang H et al . 2010 . Proteomic analysis of differentially expressed proteins in lymphoid organ of Fenneropenaeus chinensis response to Vibrio anguillarum stimulation . Fish & Shellfish Immunology , 29 ( 2 ): 186 - 194 , https://doi.org/10.1016/j.fsi.2010.03.011 https://doi.org/10.1016/j.fsi.2010.03.011 .

Zhao D X , Chen L Q , Qin C J et al . 2010 . A delta-class glutathione transferase from the Chinese mitten crab Eriocheir sinensis : cDNA cloning, characterization and mRNA expression . Fish & Shellfish Immunology , 29 ( 4 ): 698 - 703 , https://doi.org/10.1016/j.fsi.2010.06.002 https://doi.org/10.1016/j.fsi.2010.06.002 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain

Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons

Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Research on Unsteady Characteristics ofUnderwater Supersonic Gas Jet

Related Author

Boxin ZENG

Taiwei DONG

Yanting XIA

Shun YANG

Hui FEI

Mengmeng HUANG

Hao CHEN

Mengna LI

Related Institution

Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University

College of Life Sciences and Medicine, Zhejiang Sci-Tech University

Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology

Center for Ocean Mega-Science, Chinese Academy of Sciences

University of Chinese Academy of Sciences

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis

DOI：

摘要

Abstract

关键词

Keywords

references