RT-qPCR normalization of reference genes in different life-history stages of <italic style="font-style: italic">Gracilaria</italic> <italic style="font-style: italic">vermiculophylla</italic> (Rhodophyta)

Yingyue ZHANG; Jinxin YANG; Ze YANG; Cong QI; Di XU

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RT-qPCR normalization of reference genes in different life-history stages of Gracilaria vermiculophylla (Rhodophyta)

Biology | Updated：2024-10-14

- RT-qPCR normalization of reference genes in different life-history stages of Gracilaria vermiculophylla (Rhodophyta)
- Journal of Oceanology and Limnology Vol. 41, Issue 5, Pages: 1910-1917(2023)
- Affiliations：
  
  Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  dixu@ouc.edu.cn
- Funds：
- DOI：
  CLC：
- Received：25 April 2022，
  
  Accepted：13 July 2022，
  
  Online First：15 August 2022，
  
  Published：01 September 2023
- Accepted：
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ZHANG Yingyue,YANG Jinxin,YANG Ze,et al.RT-qPCR normalization of reference genes in different life-history stages of Gracilariavermiculophylla (Rhodophyta)[J].Journal of Oceanology and Limnology,2023,41(05):1910-1917.
DOI：

ZHANG Yingyue,YANG Jinxin,YANG Ze,et al.RT-qPCR normalization of reference genes in different life-history stages of Gracilariavermiculophylla (Rhodophyta)[J].Journal of Oceanology and Limnology,2023,41(05):1910-1917. DOI：

摘要

Abstract

The quantitative realtime polymerase chain reaction (RT-qPCR) is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data normalization. We evaluated the expressions of 8 housekeeping gen

18S ribosomal rDNA

(

18S rDNA

)

28S

ribosomal

rDNA

(

28S rDNA

)

rubisco

large

subunit

(

rbc

)

actin

(

ACT

)

glyceraldehyde

phosphate

dehydrogenase

(

GAPDH

)

elongation

factor

(

EF1

)

tubulin

(

TubB

)

and

phycoerythrin

(

PEB

)

to select the suitable reference genes for different life-history stages (tetrasporophyte

carposporophyte

and male/female gametophyte) of

Gracilaria

vermiculophylla

by absolute quantitative method. Softwares geNorm and BestKeeper were used to verify the results acquired from copy number analysis. Results show that the expression of identified reference genes varied in comparing groups composed of different type of life stages. It is suggested that

18S rDNA

and

TubB

could be used for highly complex samples composed of mixed ploidy and phas

18S rDNA

and

28S rDNA

were also preferred for using among the matured isomorphic samples. But for samples with different maturities

TubB

and

ACT

were recommended for tetrasporophytes and gametophytes respectively.

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Keywords

references

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RT-qPCR normalization of reference genes in different life-history stages of Gracilaria vermiculophylla (Rhodophyta)

DOI：

摘要

Abstract

关键词

Keywords

references