Transcriptomes of <italic style="font-style: italic">Litopenaeus</italic> <italic style="font-style: italic">vannamei</italic> reveal modulation of antioxidant system induced by dietary archaeal carotenoids

Wei XIE; Guoru DU; Honggang DENG; Yingchao MA; Meirong GAO; Hu DUAN; Sung YIK YEONG; Liying SUI

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Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

Aquaculture and Fisheries | Updated：2024-10-14

- Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids
- Journal of Oceanology and Limnology Vol. 41, Issue 5, Pages: 2010-2019(2023)
- Affiliations：
  
  1.State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China
  2.Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
  3.Institute of Marine Biotechnology (IMB), Universiti Malaysia Terengganu (UMT), Kuala Nerus, Terengganu 21030, Malaysia
- Author bio：
  
  suily@tust.edu.cn
- Funds：
- DOI：
  CLC：
- Received：05 June 2022，
  
  Accepted：11 July 2022，
  
  Online First：17 August 2022，
  
  Published：01 September 2023
- Accepted：
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XIE Wei,DU Guoru,DENG Honggang,et al.Transcriptomes of ,Litopenaeus,vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids[J].Journal of Oceanology and Limnology,2023,41(05):2010-2019.
DOI：

XIE Wei,DU Guoru,DENG Honggang,et al.Transcriptomes of ,Litopenaeus,vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids[J].Journal of Oceanology and Limnology,2023,41(05):2010-2019. DOI：

摘要

Abstract

Oxidative stress induced by factors such as ammonia nitrogen has become a major issue in shrimp farming. The effects of carotenoids on the growth and antioxidant capability of

Litopenaeus

vannamei

juveniles were investigated in this study using dietary archaeal carotenoids supplementation. For four weeks

shrimp were given diets containing 0 mg/kg (Ctrl) and 55.98 mg/kg (Car) archaeal carotenoids. Dietary archaeal carotenoids significantly enhanced the astaxanthin content in shrimp muscles and carapaces

as well as the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity (

0.05). The malonaldehyde (MDA) content in Car group significantly decreased (

0.05). The transcriptome analysis was conducted to determine the molecular processes in response to archaeal carotenoids supplementation. A total of 1 536 differentially expressed genes (DEG

s) were detected

including 538 upregulated DEGs and 998 downregulated DEGs. GO functional enrichment analysis between Ctrl and Car indicated that 26 GO terms including extracellular region

metabolic process

and proteolysis were enriched. The KEGG pathway enrichment analysis revealed that the amino sugar and nucleotide sugar metabolism

cysteine and methionine metabolism

glycine serine and threonine metabolism

and amino acid biosynthesis were enriched. Archaeal carotenoids influenced the expression of several important genes involved in reactive oxygen species (ROS) generation

Nrf2 signaling

and antioxidant enzymes. Seven DEGs were chosen to confirm the RNA-Seq data using qRT-PCR. The genes and pathways discovered in this work assist to elucidate the molecular processes through which archaeal carotenoid enhances

vannamei

antioxidative system.

关键词

Keywords

references

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Related Author

Wei XIE

Guoru DU

Honggang DENG

Yingchao MA

Meirong GAO

Hu DUAN

Liying SUI

Hailun FU

Related Institution

Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Ministry of Education, Tianjin University of Science and Technology

School of Marine Science and Engineering, Qingdao Agricultural University

State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Oceanology, Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center

Marine Sciences Research Institute of Shandong Province, National Oceanographic Center

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Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

DOI：

摘要

Abstract

关键词

Keywords

references