Characterization of the sphingolipid profiling of <italic style="font-style: italic">Emiliania huxleyi</italic> against virus infection

Jingwen LIU; Jingjing GAO; Enquan ZHANG; Hanrui JIANG; Guiling LI; Jian LI; Jun ZENG; Daren WU

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Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection

Biology | Updated：2024-10-14

- Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection
- Journal of Oceanology and Limnology Vol. 41, Issue 4, Pages: 1547-1557(2023)
- Affiliations：
  
  College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China
- Author bio：
  
  zjxm69602@yeah.net
  darrenwu20@163.com
- Funds：
- DOI：
  CLC：
- Received：07 January 2022，
  
  Accepted：05 May 2022，
  
  Online First：27 May 2022，
  
  Published：01 July 2023
- Accepted：
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LIU Jingwen,GAO Jingjing,ZHANG Enquan,et al.Characterization of the sphingolipid profiling of ,Emiliania huxleyi against virus infection[J].Journal of Oceanology and Limnology,2023,41(04):1547-1557.
DOI：

LIU Jingwen,GAO Jingjing,ZHANG Enquan,et al.Characterization of the sphingolipid profiling of ,Emiliania huxleyi against virus infection[J].Journal of Oceanology and Limnology,2023,41(04):1547-1557. DOI：

摘要

Abstract

Lipidomics approach by UPLC-Q-Exactive-MS was used for the identification

quantification

comparison

and characterization of sphingolipids in virus infected marine

Emiliania huxleyi

BOF92 cells. The results show that 16 significantly changed sphingolipids (including Cer

CerG1

and SPHm) were identified during viral infection. Our data confirmed previously recognized facts that viral infection led to a shift toward virus-specific sphingolipids

which is consistent with the down-regulation of genes involved in the host de novo sphingolipid biosynthesis. Moreover

we revealed the upregulation of virus-encoded homologous genes participating in de novo sphingolipids biosynthesis and virus-specific hydroxylated long chain bases (LCBs) as phytoCer

suggesting the competitive inhibition of host sphingolipid synthesis to produce the required building blocks for viral production

replication

and assembly. Additionally

Cer 40꞉1;2

Cer 40꞉2;2 isomer

and CerG1 39꞉0;2

Cer 39꞉0;2 as novel metabolite markers might indicate the general dysfunctions in

huxleyi

in response to viral infection. Our results show that viral infection led to a profound remodeling of host sphingolipidome

by which viruses depend on the hijacking of host sphingolipid metabolism to support the viral life cycle.

关键词

Keywords

references

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Related Author

Jingjing GAO

Enquan ZHANG

Hanrui JIANG

Guiling LI

Jian LI

Jun ZENG

Daren WU

Jingwen LIU

Related Institution

College of Food and Bioengineering, Jimei University

Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering

Key Laboratory of Mariculture Ministry of Education Ocean University of China

Marine Ecology Center First Institute of Oceanography State Oceanic Administration (SOA)

Key Lab of Active Material and Modern Analysis Technology State Oceanic Administration (SOA)

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Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection

DOI：

摘要

Abstract

关键词

Keywords

references