Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom <italic style="font-style: italic">Phaeodactylum tricornutum</italic>

Yufeng YANG; Yang LU; Jianwei ZHENG; Dawei LI; Xiang WANG; Weidong YANG; Hongye LI

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Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom Phaeodactylum tricornutum

Ecology | Updated：2024-10-14

- Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom Phaeodactylum tricornutum
- Journal of Oceanology and Limnology Vol. 41, Issue 5, Pages: 1809-1820(2023)
- Affiliations：
  
  Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
- Author bio：
  
  thyli@jnu.edu.cn
- Funds：
- DOI：
  CLC：
- Received：15 April 2022，
  
  Accepted：27 May 2022，
  
  Online First：05 July 2022，
  
  Published：01 September 2023
- Accepted：
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YANG Yufeng,LU Yang,ZHENG Jianwei,et al.Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom Phaeodactylum tricornutum[J].Journal of Oceanology and Limnology,2023,41(05):1809-1820.
DOI：

YANG Yufeng,LU Yang,ZHENG Jianwei,et al.Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom Phaeodactylum tricornutum[J].Journal of Oceanology and Limnology,2023,41(05):1809-1820. DOI：

摘要

Abstract

Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis. It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels

thereby affecting plant growth and development. In this study

the effects of paclobutrazol on the model diatom

Phaeodactylum

tricornutum

were investigated. Results show that 2.5-mg/L and 10-mg/L paclobutrazol significantly inhibited the algal growth by inhibiting chlorophyll synthesis

which affects photosynthesis. The antioxidant system

including catalase (CAT) and glutathione peroxidase (GPx) was severely damaged. Chrysolaminarin content was significantly elevated and doubled up to 127 mg/g dry cell weight (DCW) by 10-mg/L paclobutrazol treatment. In combination with transcriptomic analysis

paclobutrazol was demonstrated to play a regulatory role in the accumulation of chrysolaminarin and neutral lipids.

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Keywords

references

Balamurugan S , Wang X , Wang H L et al . 2017 . Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum . Biotechnology for Biofuels , 10 ( 1 ): 97 , https://doi.org/10.1186/s13068-017-0786-0 https://doi.org/10.1186/s13068-017-0786-0 .

Bolger A M , Lohse M , Usadel B . 2014 . Trimmomatic: a flexible trimmer for Illumina sequence data . Bioinformatics , 30 ( 15 ): 2114 - 2120 , https://doi.org/10.1093/bioinformatics/btu170 https://doi.org/10.1093/bioinformatics/btu170 .

Buck J M , Sherman J , Bártulos C R et al . 2019 . Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum . Nature Communications , 10 ( 1 ): 4167 , https://doi.org/10.1038/s41467-019-12043-6 https://doi.org/10.1038/s41467-019-12043-6 .

Butler T , Kapoore R V , Vaidyanathan S . 2020 . Phaeodactylum tricornutum : a diatom cell factory . Trends in Biotechnology , 38 ( 6 ): 606 - 622 , https://doi.org/10.1016/j.tibtech.2019.12.023 https://doi.org/10.1016/j.tibtech.2019.12.023 .

Carballo C , Chronopoulou E G , Letsiou S et al . 2018 . Antioxidant capacity and immunomodulatory effects of a chrysolaminarin-enriched extract in Senegalese sole . Fish & Shellfish Immunology , 82 : 1 - 8 , https://doi.org/10.1016/j.fsi.2018.07.052 https://doi.org/10.1016/j.fsi.2018.07.052 .

Daboussi F , Leduc S , Maréchal A et al . 2014 . Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology . Nature Communications , 5 ( 1 ): 3831 , https://doi.org/10.1038/ncomms4831 https://doi.org/10.1038/ncomms4831 .

Demarty M , Morvan C , Thellier M . 1984 . Calcium and cell wall . Plant, Cell & Environment , 7 ( 6 ): 441 - 448 , https://doi.org/10.1111/j.1365-3040.1984.tb01434.x https://doi.org/10.1111/j.1365-3040.1984.tb01434.x .

DuBois M , Gilles K A , Hamilton J K et al . 1956 . Colorimetric method for determination of sugars and related substances . Analytical Chemistry , 28 ( 3 ): 350 - 356 , https://doi.org/10.1021/ac60111a017 https://doi.org/10.1021/ac60111a017 .

Durkin C A , Mock T , Armbrust E V . 2009 . Chitin in diatoms and its association with the cell wall . Eukaryotic Cell , 8 ( 7 ): 1038 - 1050 , https://doi.org/10.1128/EC.00079-09 https://doi.org/10.1128/EC.00079-09 .

Gao C F , Wang Y , Shen Y et al . 2014 . Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes . BMC Genomics , 15 ( 1 ): 582 , https://doi.org/10.1186/1471-2164-15-582 https://doi.org/10.1186/1471-2164-15-582 .

Haimovich-Dayan M , Garfinkel N , Ewe D et al . 2013 . The role of C4 metabolism in the marine diatom Phaeodactylum tricornutum . The New Phytologist , 197 ( 1 ): 177 - 185 , https://doi.org/10.1111/j.1469-8137.2012.04375.x https://doi.org/10.1111/j.1469-8137.2012.04375.x .

Halliwell B . 1987 . Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts . Chemistry and Physics of Lipids , 44 ( 2-4 ): 327 - 340 , https://doi.org/10.1016/0009-3084(87)90056-9 https://doi.org/10.1016/0009-3084(87)90056-9 .

Henrotte E , Mandiki R S N M , Prudencio A T et al . 2010 . Egg and larval quality, and egg fatty acid composition of Eurasian perch breeders ( Perca fluviatilis ) fed different dietary DHA/EPA/AA ratios . Aquaculture Research , 41 ( 9 ): e53 - e61 , https://doi.org/10.1111/j.1365-2109.2009.02455.x https://doi.org/10.1111/j.1365-2109.2009.02455.x .

Hildebrand M , Manandhar-Shrestha K , Abbriano R . 2017 . Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana : implications of reduced carbohydrate storage relative to green algae . Algal Research , 23 : 66 - 77 , https://doi.org/10.1016/j.algal.2017.01.010 https://doi.org/10.1016/j.algal.2017.01.010 .

Hua S J , Zhang Y F , Yu H S et al . 2014 . Paclobutrazol application effects on plant height, seed yield and carbohydrate metabolism in canola . International Journal of Agriculture and Biology , 16 ( 3 ): 471 - 479 .

Huang W C , Haferkamp I , Lepetit B et al . 2018 . Reduced vacuolar β-1, 3-glucan synthesis affects carbohydrate metabolism as well as plastid homeostasis and structure in Phaeodactylum tricornutum . Proceedings of the National Academy of Sciences of the United States of America , 115 ( 18 ): 4791 - 4796 , https://doi.org/10.1073/pnas.1719274115 https://doi.org/10.1073/pnas.1719274115 .

Jaleel C A , Manivannan P , Sankar B et al . 2007 . Paclobutrazol enhances photosynthesis and ajmalicine production in Catharanthus roseus . Process Biochemistry , 42 ( 11 ): 1566 - 1570 , https://doi.‍org/10.1016/j.procbio.2007.08.006 https://doi.‍org/10.1016/j.procbio.2007.08.006 . https://do 10.1016/j.procbio.2007.08.006 http://dx.doi.org/10.1016/j.procbio.2007.08.006

Jeffrey S W , Humphrey G F . 1975 . New spectrophotometric equations for determining chlorophylls a , b, c 1 and c 2 in higher plants, algae and natural phytoplankton . Biochemie und Physiologie der Pflanzen , 167 ( 2 ): 191 - 194 , https://doi.org/10.1016/S0015-3796(17)30778-3 https://doi.org/10.1016/S0015-3796(17)30778-3 .

Jian J B , Zeng D Z , Wei W et al . 2017 . The combination of RNA and protein profiling reveals the response to nitrogen depletion in Thalassiosira pseudonana . Scientific Reports , 7 ( 1 ): 8989 , https://doi.org/10.1038/s41598-017-09546-x https://doi.org/10.1038/s41598-017-09546-x .

Jin M K , Wang H , Liu H J et al . 2020 . Oxidative stress response and proteomic analysis reveal the mechanisms of toxicity of imidazolium-based ioni c liquids against Arabidopsis thaliana . Environmental Pollution , 260 : 114013 , https://doi.org/10.1016/j.envpol.2020.114013 https://doi.org/10.1016/j.envpol.2020.114013 .

Ju Z G , Ding L X , Zheng Q W et al . 2011 . Diatoms as a model system in studying lipid biosynthesis regulation . International Journal of Environmental Science and Development , 2 ( 6 ): 493 - 495 , https://doi.org/10.7763/IJESD.2011.V2.176 https://doi.org/10.7763/IJESD.2011.V2.176 .

Kamran M , Cui W W , Ahmad I et al . 2018 . Effect of paclobutrazol, a potential growth regulator on stalk mechanical strength, lignin accumulation and its relation with lodging resistance of maize . Plant Growth Regulation , 84 ( 2 ): 317 - 332 , https://doi.org/10.1007/s10725-017-0342-8 https://doi.org/10.1007/s10725-017-0342-8 .

Kim D , Paggi J M , Park C et al . 2019 . Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype . Nature Biotechnology , 37 ( 8 ): 907 - 915 , https://doi.org/10.1038/s41587-019-0201-4 https://doi.org/10.1038/s41587-019-0201-4 .

Kolde R , Kolde M R . 2015 . Package 'pheatmap' . R Package , 1 ( 7 ): 790 .

Kröger N , Bergsdorf C , Sumper M . 1994 . A new calcium binding glycoprotein family constitutes a major diatom cell wall component . The EMBO Journal , 13 ( 19 ): 4676 - 4683 , https://doi.org/10.1002/j.1460-2075.1994.tb06791.x https://doi.org/10.1002/j.1460-2075.1994.tb06791.x .

Li B , Dewey C N . 2011 . RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome . BMC Bioinformatics , 12 ( 1 ): 323 , https://doi.org/10.1186/1471-2105-12-323 https://doi.org/10.1186/1471-2105-12-323 .

Li D W , Balamurugan S , Yang Y F et al . 2019 . Transcriptional regulation of microalgae for concurrent lipid overproduction and secretion . Science Advances , 5 ( 1 ): eaau 3795 , https://doi.org/10.1126/sciadv.aau3795 https://doi.org/10.1126/sciadv.aau3795 .

Love M I , Huber W , Anders S . 2014 . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 . Genome Biology , 15 ( 12 ): 550 , https://doi.‍org/10.1186/s13059-014-0550-8 https://doi.‍org/10.1186/s13059-014-0550-8 . https://do 10.1186/s13059-014-0550-8 http://dx.doi.org/10.1186/s13059-014-0550-8

Mimouni V , Ulmann L , Pasquet V et al . 2012 . The potential of microalgae for the production of bioactive molecules of pharmaceutical interest . Current Pharmaceutical Biotechnology , 13 ( 15 ): 2733 - 2750 , https://doi.org/10.2174/138920112804724828 https://doi.org/10.2174/138920112804724828 .

Pouvreau J B , Morançais M , Taran F D R et al . 2008 . Antioxidant and free radical scavenging properties of marennine, a blue-green polyphenolic pigment from the diatom Haslea ostrearia (Gaillon/Bory) Simonsen responsible for the natural greening of cultured oysters . Journal of Agricultural and Food Chemistry , 56 ( 15 ): 6278 - 6286 , https://doi.org/10.1021/jf073187n https://doi.org/10.1021/jf073187n .

Qiao H J , Cong C , Sun C X et al . 2016 . Effect of culture conditions on growth, fatty acid composition and DHA/EPA ratio of Phaeodactylum tricornutum . Aquaculture , 452 : 311 - 317 , https://doi.org/10.1016/j.aquaculture.2015.11.011 https://doi.org/10.1016/j.aquaculture.2015.11.011 .

Rademacher W . 2015 . Plant growth regulators: backgrounds and uses in plant production . Journal of Plant Growth Regulation , 34 ( 4 ): 845 - 872 , https://doi.org/10.1007/s00344-015-9541-6 https://doi.org/10.1007/s00344-015-9541-6 .

Rawat I , Kumar R R , Mutanda T et al . 2013 . Biodiesel from microalgae: a critical evaluation from laboratory to large scale production . Applied Energy , 103 : 444 - 467 , https://doi.org/10.1016/j.apenergy.2012.10.004 https://doi.org/10.1016/j.apenergy.2012.10.004 .

Serif M , Dubois G , Finoux A L et al . 2018 . One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing . Nature Communications , 9 ( 1 ): 3924 , https://doi.org/10.1038/s41467-018-06378-9 https://doi.org/10.1038/s41467-018-06378-9 .

Sivaramakrishnan R , Incharoensakdi A . 2017 . Enhancement of lipid production in Scenedesmus sp. by UV mutagenesis and hydrogen peroxide treatment . Bioresource Technology , 235 : 366 - 370 , https://doi.org/10.1016/j.biortech.2017.03.102 https://doi.org/10.1016/j.biortech.2017.03.102 .

Sun C C , Chen S , Jin Y J et al . 2016 . Effects of t he herbicide imazethapyr on photosynthesis in PGR5- and NDH-deficient Arabidopsis thaliana at the biochemical, transcriptomic, and proteomic levels . Journal of Agricultural and Food Chemistry , 64 ( 22 ): 4497 - 4504 , https://doi.‍org/10.1021/acs.jafc.6b01699 https://doi.‍org/10.1021/acs.jafc.6b01699 . https://do 10.1021/acs.jafc.6b01699 http://dx.doi.org/10.1021/acs.jafc.6b01699

Swietlik D , Miller S S . 1984 . The effect of paclobutrazol on the growth and uptake of Ca and K by apple seedlings . Journal of Plant Nutrition , 7 ( 11 ): 1555 - 1565 , https://doi.org/10.1080/01904168409363302 https://doi.org/10.1080/01904168409363302 .

Wang X , Dong H P , Wei W et al . 2018 . Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in Phaeodactylum tricornutum . Biotechnology for Biofuels , 11 ( 1 ): 318 , https://doi.org/10.1186/s13068-018-1317-3 https://doi.org/10.1186/s13068-018-1317-3 .

Wieland W F , Wample R L . 1985 . Effects of paclobutrazol on growth, photosynthesis and carbohydrate content of 'dDlicious' apples . Scientia Horticulturae , 26 ( 2 ): 139 - 147 , https://doi.org/10.1016/0304-4238(85)90006-8 https://doi.org/10.1016/0304-4238(85)90006-8 .

Xue J , Balamurugan S , Li T et al . 2021 . Biotechnological approaches to enhance biofuel producing potential of microalgae . Fuel , 302 : 121169 , https://doi.org/10.1016/j.fuel.2021.121169 https://doi.org/10.1016/j.fuel.2021.121169 .

Xue J , Niu Y F , Huang T et al . 2015 . Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation . Metabolic Engineering , 27 : 1 - 9 , https://doi.org/10.1016/j.ymben.2014.10.002 https://doi.org/10.1016/j.ymben.2014.10.002 .

Yang R Q , Wei D , Xie J . 2020 . Diatoms as cell factories for high-value products: chrysolaminarin, eicosapentaenoic acid, and fucoxanthin . Critical Reviews in Biotechnology , 40 ( 7 ): 993 - 1009 , https://doi.org/10.1080/07388551.2020.1805402 https://doi.org/10.1080/07388551.2020.1805402 .

Yang Y F , Li D W , Chen T T et al . 2019 . Overproduction of bioactive algal chrysolaminarin by the critical carbon flux regulator phosphoglucomutase . Biotechnology Journal , 14 ( 3 ): 1800220 , https://doi.org/10.1002/biot.201800220 https://doi.org/10.1002/biot.201800220 .

Yang Z K , Niu Y F , Ma Y H et al . 2013 . Molecular and cellular mechanisms of neutral lipid accumulation in diatom following nitrogen deprivation . Biotechnology for Biofuels , 6 ( 1 ): 67 , https://doi.org/10.1186/1754-6834-6-67 https://doi.org/10.1186/1754-6834-6-67 .

Yang Z K , Zheng J W , Niu Y F et al . 2014 . Systems‐level analysis of the metabolic responses of the diatom Phaeodactylum tricornutum to phosphorus stress . Environmental Microbiology , 16 ( 6 ): 1793 - 1807 , https://doi.org/10.1111/1462-2920.12411 https://doi.org/10.1111/1462-2920.12411 .

Yu T , Zhou Y J , Huang M T et al . 2018 . Reprogramming yeast metabolism from alcoholic fermentation to lipogenesis . Cell , 174 ( 6 ): 1549 - 1558 , https://doi.org/10.1016/j.cell.2018.07.013 https://doi.org/10.1016/j.cell.2018.07.013 .

Zheng J W , Li D W , Lu Y et al . 2016 . Molecular exploration of algal interaction between the diatom Phaeodactylum tricornutum and the dinoflagellate Alexandrium tamarense . Algal Research , 17 : 132 - 141 , https://doi.org/10.1016/j.algal.2016.04.019 https://doi.org/10.1016/j.algal.2016.04.019 .

Zheng R R , Wu Y , Xia Y P . 2012 . Chlorocholine chloride and paclobutrazol treatments promote carbohydrate accumulation in bulbs of Lilium Oriental hybrids 'Sorbonne' . Journal of Zhejiang University Science B , 13 ( 2 ): 136 - 144 , https://doi.org/10.1631/jzus.B1000425 https://doi.org/10.1631/jzus.B1000425 .

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Related Institution

Hong Kong Branch of Southern Marine Science & Engineering Guangdong Laboratory, The Hong Kong University of Science and Technology

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School of Environmental and Geographical Sciences, Shanghai Normal University

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Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom Phaeodactylum tricornutum

DOI：

摘要

Abstract

关键词

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