Metabolomics of astaxanthin hyperaccumulation in <italic style="font-style: italic">Haematococcus</italic> <italic style="font-style: italic">pluvialis</italic> under high light stress

Yong DOU; Jiayi LI; Wenli ZHOU

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Metabolomics of astaxanthin hyperaccumulation in Haematococcus pluvialis under high light stress

Biology | Updated：2024-10-14

- Metabolomics of astaxanthin hyperaccumulation in Haematococcus pluvialis under high light stress
- Journal of Oceanology and Limnology Vol. 41, Issue 5, Pages: 1876-1886(2023)
- Affiliations：
  
  1.Tianjin Agricultural University, College of Fisheries, Tianjin Key Lab for Aquaculture Ecology and Cultivation, Tianjin 300384, China
  2.Tianjin Modern Tianjiao Agricultural Technology Co., Ltd., Tianjin Key Laboratory for Green and Ecological Forage, Tianjin 301800, China
- Author bio：
  
  saz0908@126.com
- Funds：
- DOI：
  CLC：
- Received：03 March 2022，
  
  Accepted：18 April 2022，
  
  Online First：17 June 2022，
  
  Published：01 September 2023
- Accepted：
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DOU Yong,LI Jiayi,ZHOU Wenli.Metabolomics of astaxanthin hyperaccumulation in Haematococcuspluvialis under high light stress[J].Journal of Oceanology and Limnology,2023,41(05):1876-1886.
DOI：

DOU Yong,LI Jiayi,ZHOU Wenli.Metabolomics of astaxanthin hyperaccumulation in Haematococcuspluvialis under high light stress[J].Journal of Oceanology and Limnology,2023,41(05):1876-1886. DOI：

摘要

Abstract

Variation in metabolite profiles of

Haematococcus

pluvialis

(a type of unicellular green algal) under light stress is a key issue of study at the present. To investigate the effect of light intensity on accumulation of astaxa

nthin in

pluvialis

a 26-day batch culture experiment of

pluvialis

under the light intensity levels at 73

127

182

236

and 291 μmol/(m

·s) was conducted. Therefore

the optimal light intensity and the corresponding metabolic pathways of accumulation in

pluvialis

were determined. Results show that 236 μmol/(m

·s) was the optimum light intensity to induce astaxanthin accumulation

at which a maximum content of 9.01 mg/L was achieved on Day 24. A total of 132 metabolites were identified and quantified

of which 38 differential metabolites were highlighted and classified

including 3 fatty acids or intermediates

5 amino acids or derivatives

5 carbohydrates or intermediates

16 nucleoside derivatives

and 9 other metabolites using LC-MS/MS technique. Subsequently

16 statistically significant differential metabolic pathways were enriched and annotated based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis between the control and the 236 μmol/(m

·s) treatment group (

0.05). In addition

the bioprocesses included cellular basal metabolism and signaling systems

such as carbohydrate metabolism

amino acid metabolism

glycerol and derivatives metabolism

nucleotide and derivative metabolism

and inositol phosphate metabolism were activated and regulated under strong light stress conditions. Moreover

4 hub metabolites containing D-glucose-6-phosphate

L-tyrosine

glycerol-3-phosphate

and L-glutamine were identified

based on which the associated metabolic network was constructed. The study provided a metabolomic view of astaxanthin accumulation in

pluvialis

under strong light stress.

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Keywords

references

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Metabolomics of astaxanthin hyperaccumulation in Haematococcus pluvialis under high light stress

DOI：

摘要

Abstract

关键词

Keywords

references