Characterization of <italic style="font-style: italic">Wnt</italic> genes in <italic style="font-style: italic">Argopecten</italic> scallops and their involvement in response to different temperature stresses in “Bohai Red” scallops

Caihui WANG; Shuhua LEI; Min CHEN; Junhao NING; Xia LU; Jinsheng ZHANG; Bo LIU; Chunde WANG

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Characterization of Wnt genes in Argopecten scallops and their involvement in response to different temperature stresses in “Bohai Red” scallops

Biology | Updated：2024-10-12

- Characterization of Wnt genes in Argopecten scallops and their involvement in response to different temperature stresses in “Bohai Red” scallops
- Journal of Oceanology and Limnology Vol. 41, Issue 3, Pages: 1122-1132(2023)
- Affiliations：
  
  1. Qingdao Agricultural University, Qingdao 266109, China
  2. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  3. Zhaoyuan Xiadian Agricultural Technology Promotion Station, Yantai 265415, China
- Author bio：
  
  liubomusic@126.com
  chundewang2007@163.com
- Funds：
- DOI：
  CLC：
- Received：30 December 2021，
  
  Published：01 May 2023
- Accepted：
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WANG Caihui,LEI Shuhua,CHEN Min,et al.Characterization of Wnt genes in Argopecten scallops and their involvement in response to different temperature stresses in “Bohai Red” scallops[J].Journal of Oceanology and Limnology,2023,41(03):1122-1132.
DOI：

WANG Caihui,LEI Shuhua,CHEN Min,et al.Characterization of Wnt genes in Argopecten scallops and their involvement in response to different temperature stresses in “Bohai Red” scallops[J].Journal of Oceanology and Limnology,2023,41(03):1122-1132. DOI：

摘要

Abstract

As “Bohai Red” scallops were originated from the hybrids between the Peruvian scallop (

Argopecten purpuratus

) and the bay scallop (

Argopecten irradians

) northern subspecies (

Argopecten irradians irradians

). Twelve

Wnt

members were identified from the two subspecies of bay scallop

and 13

Wnt

genes were found in the genome of the Peruvian scallop. Protein structure analyses showed that most

Wnt

genes poses all 5 conserved motifs except

Wnt1

Wnt2

Wnt6

and

Wnt9

in the bay scallops and

Wnt

2 and

Wnt9

in the Peruvian scallop. Unexpectedly

Wnt8

gene was present while

Wnt3

was absent in both the bay scallops and the Peruvian scallop. Phylogenetic analysis revealed that

Wnt3

might have disappeared in the early evolution of mollusks. The expression profile of

Wnt

genes in the “Bohai Red” exposed to different temperatures was examined by qRT-PCR. Results show that expression of Wnt genes responded differentially to temperature changes. The

Wnt

genes such as

Wnt1

Wnt6

Wnt7

Wnt11

and

WntA

that responded slowly to low and high temperature stresses may be related to the maintenance of basic homeostasis. Other

Wnt

genes such as

Wnt4

Wnt9

Wnt5

and

Wnt2

that responded rapidly to low temperature may play an important role in organismal protection against low temperature stress. And yet some

Wnt

genes including

Wnt10

Wnt16

and

Wnt8

that responded quickly to high temperature stress may play key roles in response to high temperature stress. The results provide new insights into the evolution and function of

Wnt

genes in bivalves and eventually benefit culture of “Bohai Red” scallops.

关键词

Keywords

references

Abele D , Puntarulo S . 2004 . Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish . Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology , 138 ( 4 ): 405 - 415 , https://doi.org/10.1016/j.cbpb.2004.05.013 https://doi.org/10.1016/j.cbpb.2004.05.013 .

Almroth B C , Asker N , Wassmur B et al . 2015 . Warmer water temperature results in oxidative damage in an Antarctic fish, the bald notothen . Journal of Experimental Marine Biology and Ecology , 468 : 130 - 137 , https://doi.org/10.1016/j.jembe.2015.02.018 https://doi.org/10.1016/j.jembe.2015.02.018 .

Almroth B C , de Souza K B , Jönsson E et al . 2019 . Oxidative stress and biomarker responses in the Atlantic halibut after long term exposure to elevated CO 2 and a range of temperatures . Comparative Biochemistry and Physiology Part B : Biochemistry and Molecular Biology , 238 : 110321 , https://doi.org/10.1016/j.cbpb.2019.110321 https://doi.org/10.1016/j.cbpb.2019.110321 .

Amerongen R V . 2012 . Alternative Wnt pathways and receptors . Cold Spring Harbor Perspectives in Biology , 4 ( 10 ): a007914 , https://doi.org/10.1101/cshperspect.a007914 https://doi.org/10.1101/cshperspect.a007914 .

Bai Y T , Nie H T , Wang Z X et al . 2020 . Genome-wide identification and transcriptome-based expression profiling of Wnt gene family in Ruditapes philippinarum . Comparative Biochemistry and Physiology Part D : Genomics and Proteomics , 35 : 100709 , https://doi.org/10.1016/j.cbd.2020.100709 https://doi.org/10.1016/j.cbd.2020.100709 .

Blake N J , Shumway S E . 2006 . Chapter 17 Bay scallop and calico scallop fisheries, culture and enhancement in eastern North America . Developments in Aquaculture and Fisheries Science , 35 : 945 - 964 , https://doi.org/10.1016/S0167-9309(06)80044-X https://doi.org/10.1016/S0167-9309(06)80044-X .

Bolognesi R , Beermann A , Farzana L et al . 2008 . Tribolium Wnts: evidence for a larger repertoire in insects with overlapping expression patterns that suggest multiple redundant functions in embryogenesis . Development Genes and Evolution , 218 ( 3-4 ): 193 - 202 , https://doi.org/10.1007/s00427-007-0170-3 https://doi.org/10.1007/s00427-007-0170-3 .

Chen C J , Chen H , Zhang Y et al . 2020a . TBtools: an integrative toolkit developed for interactive analyses of big biological data . Molecular Plant , 13 ( 8 ): 1194 - 1202 , https://doi.org/10.1016/j.molp.2020.06.009 https://doi.org/10.1016/j.molp.2020.06.009 .

Chen M , Liu B , Ma B et al . 2020b . Selection of a carotenoid-rich scallop strain, QN Orange, from the inter-specific hybrids between the bay scallop and the Peruvian scallop . Aquaculture , 528 : 735513 , https://doi.org/10.1016/j.aquaculture.2020.735513 https://doi.org/10.1016/j.aquaculture.2020.735513 .

Dall W H . 1909 . The mollusca and branchiopoda. Report of the ared 19 operation Albatros . Bulletin Mollusca Comparative Zoology , 37 : 147 - 294 . https://do 10.5479/si.00963801.37-1704.147 http://dx.doi.org/10.5479/si.00963801.37-1704.147

Doney S C , Ruckelshaus M , Duffy J E et al . 2012 . Climate change impacts on marine ecosystems . Annual Review of Marine Science , 4 : 11 - 37 , https://doi.org/10.1146/annurev-marine-041911-111611 https://doi.org/10.1146/annurev-marine-041911-111611 .

Dong Z G , Mao S , Chen Y H et al . 2019 . Effects of air-exposure stress on the survival rate and physiology of the swimming crab Portunus trituberculatus . Aquaculture , 500 : 429 - 434 , https://doi.org/10.1016/j.aquaculture.2018.10.049 https://doi.org/10.1016/j.aquaculture.2018.10.049 .

Dzamba B J , Jakab K R , Marsden M et al . 2009 . Cadherin adhesion, tissue tension, and noncanonical Wnt signaling regulate fibronectin matrix organization . Developmental Cell , 16 ( 3 ): 421 - 432 , https://doi.org/10.1016/j.devcel.2009.01.008 https://doi.org/10.1016/j.devcel.2009.01.008 .

Estabrooks S L . 2007 . The possible role of telomeres in the short life span of the bay scallop, Argopecten irradians irradians (Lamarck 1819 ). Journal of Shellfish Research , 26 ( 2 ): 307 - 313 , https://doi.org/10.2983/0730-8000(2007)26 https://doi.org/10.2983/0730-8000(2007)26 [307:TPROTI ] 2.0.CO; 2 .

Freese J L , Pino D , Pleasure S J . 2010 . Wnt signaling in development and disease . Neurobiology of Disease , 38 ( 2 ): 148 - 153 , https://doi.org/10.1016/j.nbd.2009.09.003 https://doi.org/10.1016/j.nbd.2009.09.003 .

Garriock R J , Warkman A S , Meadows S M et al . 2007 . Census of vertebrate Wnt genes: isolation and developmental expression of Xenopus Wnt2, Wnt3, Wnt 9 a, Wnt9b, Wnt 10 a, and Wnt16. Developmental Dynamics, 236 ( 5 ): 1249 - 1258 , https://doi.org/10.1002/dvdy.21156 https://doi.org/10.1002/dvdy.21156 .

Heuer R M , Grosell M . 2014 . Physiological impacts of elevated carbon dioxide and ocean acidification on fish . American Journal of Physiology : Regulatory, Integrative and Comparative Physiology , 307 ( 9 ): R1061 - R1084 , https://doi.org/10.1152/ajpregu.00064.2014 https://doi.org/10.1152/ajpregu.00064.2014 .

Karner C , Wharton K A , Carroll T J . 2006 . Apical-basal polarity, Wnt signaling and vertebrate organogenesis . Seminars in Cell & Developmental Biology , 17 ( 2 ): 214 - 222 , https://doi.org/10.1016/j.semcdb.2006.05.007 https://doi.org/10.1016/j.semcdb.2006.05.007 .

Kumar S , Stecher G , Tamura K . 2016 . MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets . Molecular Biology and Evolution , 33 ( 7 ): 1870 - 1874 , https://doi.org/ 10.1093/molbev/msw054 https://doi.org/10.1093/molbev/msw054 .

Kusserow A , Pang K , Sturm C et al . 2005 . Unexpected complexity of the Wnt gene family in a sea anemone . Nature , 433 ( 7022 ): 156 - 160 , https://doi.org/10.1038/nature03158 https://doi.org/10.1038/nature03158 .

Lengfeld T , Watanabe H , Simakov O et al . 2009 . Multiple Wnts are involved in Hydra organizer formation and regeneration . Developmental Biology , 330 ( 1 ): 186 - 199 , https://doi.org/10.1016/j.ydbio.2009.02.004 https://doi.org/10.1016/j.ydbio.2009.02.004 .

Li C , Liu X , Liu B et al . 2018 . Draft genome of the Peruvian scallop Argopecten purpuratus . GigaScience , 7 ( 4 ): 1 - 6 , https://doi.org/10.1093/gigascience/giy031 https://doi.org/10.1093/gigascience/giy031 .

Liu S N , Chen X M , Lian S S et al . 2019 . Genome-wide identification and expression profiling of the Wnt gene family in three bivalve molluscs . Comparative Biochemistry and Physiology Part D : Genomics and Proteomics , 29 : 299 - 307 , https://doi.org/10.1016/j.cbd.2019.01.008 https://doi.org/10.1016/j.cbd.2019.01.008 .

Liu X , Li C , Chen M et al . 2020 . Draft genomes of two Atlantic bay scallop subspecies Argopecten irradians irradians and A . i . concentricus . Scientific Data , 7 ( 1 ): 99 , https://doi.org/.10.1038/s41597-020-0441-7 https://doi.org/.10.1038/s41597-020-0441-7 .

Moraga D , Avendaño M , Peña J et al . 2001 . Genetic and morphological differentiation between two pectinid populations of Argopecten purpuratus from the northern Chilean coast . Estudios Oceanologicos , 20 : 51 - 60 .

Ning J H , Zou D S , Lu X et al . 2021 . Transcriptomic analyses provide insights into the adaptive responses to heat stress in the ark shells, Scapharca subcrenata . Comparative Biochemistry and Physiology Part D : Genomics and Proteomics , 38 : 100813 , https://doi.org/ 10.1016/j.cbd.2021.100813 https://doi.org/10.1016/j.cbd.2021.100813 .

Nusse R , Varmus H E . 1982 . Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome . Cell , 31 ( 1 ): 99 - 109 , https://doi.org/10.1016/0092-8674(82)90409-3 https://doi.org/10.1016/0092-8674(82)90409-3 .

Sajjanar B , Siengdee P , Trakooljul N et al . 2019 . Cross-talk between energy metabolism and epigenetics during temperature stress response in C 2 C 12 myoblasts. International Journal of Hyperthermia , 36 ( 1 ): 775 - 783 , https://doi.org/10.1080/02656736.2019.1639834 https://doi.org/10.1080/02656736.2019.1639834 .

Sullivan J C , Ryan J F , Mullikin J C et al . 2007 . Conserved and novel Wnt clusters in the basal eumetazoan Nematostella vectensis . Development Genes and Evolution , 217 ( 3 ): 235 - 239 , https://doi.org/10.1007/s00427-007-0136-5 https://doi.org/10.1007/s00427-007-0136-5 .

Wang C D , Liu B , Liu X et al . 2017 . Selection of a new scallop strain, the Bohai Red, from the hybrid between the bay scallop and the Peruvian scallop . Aquaculture , 479 : 250 - 255 , https://doi.org/10.1016/j.aquaculture.2017.05.045 https://doi.org/10.1016/j.aquaculture.2017.05.045 .

Wang C D , Liu B Z , Li J Q et al . 2011 . Introduction of the Peruvian scallop and its hybridization with the bay scallop in China . Aquaculture , 310 ( 3-4 ): 380 - 387 , https://doi.org/10.1016/j.aquaculture.2010.11.014 https://doi.org/10.1016/j.aquaculture.2010.11.014 .

Wang Y P , Guo X M . 2004 . Chromosomal rearrangement in Pectinidae revealed by rRNA loci and implications for bivalve evolution . The Biological Bulletin , 207 ( 3 ): 247 - 256 , https://doi.org/10.2307/1543213 https://doi.org/10.2307/1543213 .

Woll P S , Morris J K , Painschab M S et al . 2008 . Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells . Blood , 111 ( 1 ): 122 - 131 , https://doi.org/10.1182/blood-2007-04-084186 https://doi.org/10.1182/blood-2007-04-084186 .

Xu D X , Wang C D , Liu B et al . 2019 . Selection of a new scallop strain, QN-2, from the backcross of Peruvian scallop×bay scallop F 1 hybrids with bay scallops . Aquaculture Research , 50 ( 12 ): 3692 - 3699 , https://doi.org/10.1111/are.14329 https://doi.org/10.1111/are.14329 .

Zhou Z , Liu Z Q , Wang L G et al . 2019 . Oxidative stress, apoptosis activation and symbiosis disruption in giant clam Tridacna crocea under high temperature . Fish & Shellfish Immunology , 84 : 451 - 457 , https://doi.org/10.1016/j.fsi.2018.10.033 https://doi.org/10.1016/j.fsi.2018.10.033 .

Zou D S , Ning J H , Lu X et al . 2021 . Physiological and transcriptional responses to acute and chronic thermal stress in the ark shell Scapharca subcrenata . Frontiers in Marine Science , 8 : 739662 , https://doi.org/10.3389/fmars.2021.739662 https://doi.org/10.3389/fmars.2021.739662 .

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⁰

Characterization of Wnt genes in Argopecten scallops and their involvement in response to different temperature stresses in “Bohai Red” scallops

DOI：

摘要

Abstract

关键词

Keywords

references