The ontogeny of acidic digestive tract in larval turbot <italic style="font-style: italic">Scophthalmus maximus</italic> based on H<sup>+</sup>/K<sup>+</sup>-ATPase and pepsinogen

Yanfeng WANG; Shulan LIU; Teng GUO; Shihong XU; Qinghua LIU; Zhizhong XIAO; Daoyuan MA; Jun LI; Liang CHI

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The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H⁺/K⁺-ATPase and pepsinogen

Biology | Updated：2024-10-12

- The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H⁺/K⁺-ATPase and pepsinogen
- Journal of Oceanology and Limnology Vol. 41, Issue 3, Pages: 1100-1108(2023)
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Centre for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Qingdao Agricultural University, Qingdao 266109, China
  3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  4.Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou 511458, China
  5.Conson Oriental (Yantai) Recirculating Aquaculture Technology Co., Ltd., Yantai 264000, China
- Author bio：
  
  junli@qdio.ac.cn
  lchi@qau.edu.cn
- Funds：
- DOI：
  CLC：
- Received：03 November 2021，
  
  Published：01 May 2023
- Accepted：
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WANG Yanfeng,LIU Shulan,GUO Teng,et al.The ontogeny of acidic digestive tract in larval turbot ,Scophthalmus maximus based on H,⁺/K,⁺-ATPase and pepsinogen[J].Journal of Oceanology and Limnology,2023,41(03):1100-1108.
DOI：

WANG Yanfeng,LIU Shulan,GUO Teng,et al.The ontogeny of acidic digestive tract in larval turbot ,Scophthalmus maximus based on H,⁺/K,⁺-ATPase and pepsinogen[J].Journal of Oceanology and Limnology,2023,41(03):1100-1108. DOI：

摘要

Abstract

Acidic digestion is an important digestive process of marine fish. In fish stomach

two enzymes are involved in the secretion of hydrochloric acid (HCl) an

d proteomic digestion: H

-ATPase and pepsinogen. However

the starting of digestive function in fish is still unclear. To reveal the details of acidic digestion of turbot

Scophthalmus

maximus

in early development

a 40 day of turbot larvae culture was conducted. The H

-ATPase gene from the turbot

maximus

(

-ATPase) was identified and characterized. Based on our previous discription on pepsinogen of turbot

maximus

we combined pepsinogen and H

-ATPase and analyzed the mechanism of acidic digestion in turbot. Results show that the spatial and temporal expression profiles of H

-ATPase agreed with pepsinogen A and C in turbot

indicating a synergetic action between H

-ATPase and pepsinogen during the acidic digestion process. In addition

the turbot juveniles showed a faster growth after the expressions of H

-ATPase gene and pepsinogen gene

demonstrating that pepsin had a higher digestive efficiency

for which a compound diet should be provided to the fish from Day 22 onward. This study provided a reference for biology research and aquaculture of turbot and other marine fishes.

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references

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Related Institution

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The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H+/K+-ATPase and pepsinogen

DOI：

摘要

Abstract

关键词

Keywords

references

The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H⁺/K⁺-ATPase and pepsinogen