Eukaryotic food sources analysis in situ of tropical common sea cucumber <italic style="font-style: italic">Holothuria leucospilota</italic> based on 18S rRNA gene high-throughput sequencing

Yue ZHANG; Fei GAO; Qiang XU; Yanan WANG; Haiqing WANG; Aimin WANG

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Eukaryotic food sources analysis in situ of tropical common sea cucumber Holothuria leucospilota based on 18S rRNA gene high-throughput sequencing

Aquaculture and Fisheries | Updated：2024-10-12

- Eukaryotic food sources analysis in situ of tropical common sea cucumber Holothuria leucospilota based on 18S rRNA gene high-throughput sequencing
- Journal of Oceanology and Limnology Vol. 41, Issue 3, Pages: 1173-1186(2023)
- Affiliations：
  
  1.State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
  2.College of Marine Science, Hainan University, Haikou 570228, China
- Author bio：
  
  gaofeicas@126.com
- Funds：
- DOI：
  CLC：
- Received：13 September 2021，
  
  Published：01 May 2023
- Accepted：
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ZHANG Yue,GAO Fei,XU Qiang,et al.Eukaryotic food sources analysis in situ of tropical common sea cucumber Holothuria leucospilota based on 18S rRNA gene high-throughput sequencing[J].Journal of Oceanology and Limnology,2023,41(03):1173-1186.
DOI：

ZHANG Yue,GAO Fei,XU Qiang,et al.Eukaryotic food sources analysis in situ of tropical common sea cucumber Holothuria leucospilota based on 18S rRNA gene high-throughput sequencing[J].Journal of Oceanology and Limnology,2023,41(03):1173-1186. DOI：

摘要

Abstract

Sea cucumber

Holothuria leucospilota

is one of the most widespread tropical holothurian species. In this study

eukaryotic organism composition in foregut and hindgut contents of

leucospilota

and surrounding sediments was assessed by 18S rRNA gene high-throughput sequencing. Eukaryon richness and diversity in the habitat sediments were significantly higher than those in foregut and hindgut contents of the sea cucumbers (

0.05). The foregut content group

hindgut content group

and marine sediment group sequences were respectively assigned to 18.20±1.32

19.40±1.03

and 21.80±0.37 phyla. In the foregut contents

Nematoda (20.18%±9.59%)

Mollusca (16.12%±10.49%)

Chlorophyta (10.04%± 4.85%)

Annelida (8.72%±10.93%)

Streptophyta (8.46%±4.65%)

and Diatomea (5.99%±2.01%) were the predominant phyla

which showed the eukaryotic food sources of

leucospilota

were primarily belong to the above phyla. The predominant phyla in the hindgut contents were Streptophyta (45.55%± 17.32%)

Mollusca (4.93%±4.82%)

Arthropoda (5.37%±3.08%)

Diatomea (3.88%±2.34%)

and Chlorophyta (3.79%±1.59%); and Annelida (37.80%±17.00%)

Arthropoda (24.49%±12.53%)

Platyhelminthes (7.14%±3.02%)

Nematoda (4.14%±0.91%)

and Diatomea (5.11%±1.35%) had large contents in the sediments. The comparatively high content of

Paris

genus in phylum Streptophyta in foregut contents indicated that land plants were one of the primary food sources of

leucospilota

however the significantly higher contents of Streptophyta in hindgut contents than tha

t in foregut contents might suggest a large part of the terrigenous detritus ingested might not be digested by

leucospilota

. UPGMA and PCoA analysis revealed that eukaryotic organism composition differed significantly between foregut contents of

leucospilota

and ambient sediments

indicating selective feeding feature of

leucospilota

. This study provided useful references for artificial feed of tropical sea cucumbers and enhanced understanding of the ecological roles of detritus-feeding macrobenthos.

关键词

Keywords

references

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Eukaryotic food sources analysis in situ of tropical common sea cucumber Holothuria leucospilota based on 18S rRNA gene high-throughput sequencing

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